Zinc in PDB 6mfi: Mim-2 Metallo-Beta-Lactamase

The structure of Mim-2 Metallo-Beta-Lactamase, PDB code: 6mfi was solved by C.Selleck, L.Guddat, G.Schenk, M.Monteiro Pedroso, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	42.83 / 1.84
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	51.672, 69.527, 76.541, 90.00, 90.00, 90.00
R / Rfree (%)	26 / 30.9

The binding sites of Zinc atom in the Mim-2 Metallo-Beta-Lactamase (pdb code 6mfi). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Mim-2 Metallo-Beta-Lactamase, PDB code: 6mfi:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Mim-2 Metallo-Beta-Lactamase


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Mim-2 Metallo-Beta-Lactamase within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn401 b:10.7 occ:1.00 O A:HOH659 2.0 11.0 1.0 OD2 A:ASP120 2.0 17.1 1.0 NE2 A:HIS259 2.1 23.5 1.0 NE2 A:HIS121 2.2 12.6 1.0 CE1 A:HIS259 2.9 23.3 1.0 CG A:ASP120 3.0 15.6 1.0 CD2 A:HIS121 3.1 12.5 1.0 CE1 A:HIS121 3.1 12.9 1.0 CD2 A:HIS259 3.2 22.7 1.0 OD1 A:ASP120 3.3 16.1 1.0 ZN A:ZN402 3.4 10.1 0.8 NE2 A:HIS116 3.8 11.3 1.0 ND1 A:HIS259 4.0 22.8 1.0 CE1 A:HIS116 4.1 11.6 1.0 ND1 A:HIS121 4.1 12.2 1.0 CG A:HIS121 4.1 11.9 1.0 CG A:HIS259 4.2 21.9 1.0 OG A:SER219 4.4 14.7 1.0 CB A:ASP120 4.4 13.9 1.0 NE2 A:HIS194 4.7 20.7 1.0 CD1 A:ILE70 4.8 13.5 1.0 O A:HOH682 4.9 49.0 1.0 ND1 A:HIS118 5.0 18.6 1.0 CZ2 A:TRP49 5.0 19.8 1.0

Zinc binding site 2 out of 2 in the Mim-2 Metallo-Beta-Lactamase


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Mim-2 Metallo-Beta-Lactamase within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn402 b:10.1 occ:0.82 O A:HOH659 2.0 11.0 1.0 ND1 A:HIS118 2.0 18.6 1.0 NE2 A:HIS194 2.0 20.7 1.0 NE2 A:HIS116 2.2 11.3 1.0 CD2 A:HIS194 2.9 20.8 1.0 CG A:HIS118 2.9 17.3 1.0 CE1 A:HIS118 2.9 18.8 1.0 CD2 A:HIS116 3.0 11.3 1.0 CE1 A:HIS194 3.1 21.6 1.0 CE1 A:HIS116 3.2 11.6 1.0 CB A:HIS118 3.3 14.4 1.0 ZN A:ZN401 3.4 10.7 1.0 NE2 A:HIS118 3.9 18.5 1.0 CD2 A:HIS118 3.9 18.0 1.0 CG A:HIS194 4.1 21.3 1.0 CG A:HIS116 4.1 11.2 1.0 ND1 A:HIS116 4.2 11.4 1.0 ND1 A:HIS194 4.2 22.0 1.0 OD1 A:ASP120 4.2 16.1 1.0 CD2 A:HIS121 4.2 12.5 1.0 NE2 A:HIS121 4.3 12.6 1.0 OD2 A:ASP120 4.7 17.1 1.0 OE1 A:GLN157 4.7 46.0 1.0 CA A:HIS118 4.8 12.3 1.0 CG2 A:THR195 4.8 15.9 1.0 O A:HOH682 4.9 49.0 1.0 CG A:ASP120 4.9 15.6 1.0 OG A:SER219 5.0 14.7 1.0

C.Selleck, D.Clayton, L.R.Gahan, N.Mitic, R.P.Mcgeary, M.M.Pedroso, L.W.Guddat, G.Schenk, M.Monteiro Pedroso. Characterization of the B3 Mbls Mim-1 and Mim-2 From Environmental Microorganisms. Chemistry V. 23 4778 2017.
ISSN: ISSN 1521-3765
PubMed: 28261912
DOI: 10.1002/CHEM.201700866