Zinc in PDB 6lac: The Structural Basis of the Beta-Carbonic Anhydrase Cafd (C39A Mutant) of the Filamentous Fungus Aspergillus Fumigatus

All present enzymatic activity of The Structural Basis of the Beta-Carbonic Anhydrase Cafd (C39A Mutant) of the Filamentous Fungus Aspergillus Fumigatus:
4.2.1.1;

The structure of The Structural Basis of the Beta-Carbonic Anhydrase Cafd (C39A Mutant) of the Filamentous Fungus Aspergillus Fumigatus, PDB code: 6lac was solved by M.S.Jiin, S.Kim, N.J.Kim, S.Hong, S.Kim, J.Sung, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	33.92 / 1.30
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	84.085, 57.505, 72.733, 90.00, 111.23, 90.00
R / Rfree (%)	19.1 / 21

The binding sites of Zinc atom in the The Structural Basis of the Beta-Carbonic Anhydrase Cafd (C39A Mutant) of the Filamentous Fungus Aspergillus Fumigatus (pdb code 6lac). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the The Structural Basis of the Beta-Carbonic Anhydrase Cafd (C39A Mutant) of the Filamentous Fungus Aspergillus Fumigatus, PDB code: 6lac:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the The Structural Basis of the Beta-Carbonic Anhydrase Cafd (C39A Mutant) of the Filamentous Fungus Aspergillus Fumigatus


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of The Structural Basis of the Beta-Carbonic Anhydrase Cafd (C39A Mutant) of the Filamentous Fungus Aspergillus Fumigatus within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn201 b:10.2 occ:1.00 OD1 A:ASP36 1.9 10.9 1.0 NE2 A:HIS89 2.1 11.2 1.0 SG A:CYS34 2.3 9.8 1.0 SG A:CYS92 2.3 11.4 1.0 CG A:ASP36 3.0 10.2 1.0 CE1 A:HIS89 3.0 10.4 1.0 CD2 A:HIS89 3.1 10.0 1.0 CB A:CYS34 3.1 9.4 1.0 CB A:CYS92 3.3 11.6 1.0 OD2 A:ASP36 3.4 12.9 1.0 CA A:CYS92 3.6 12.6 1.0 ND1 A:HIS89 4.1 10.0 1.0 O A:HOH311 4.1 10.4 1.0 N A:ASP36 4.2 9.6 1.0 CB A:ASP36 4.2 10.0 1.0 CG A:HIS89 4.2 9.2 1.0 C A:ASP36 4.2 10.3 1.0 CA A:ASP36 4.4 10.2 1.0 N A:SER37 4.4 9.7 1.0 N A:HIS93 4.4 13.8 1.0 C A:CYS92 4.4 13.0 1.0 CA A:CYS34 4.5 9.1 1.0 O A:ASP36 4.7 11.7 1.0 N A:ILE94 4.7 16.5 1.0 C A:CYS34 4.7 8.4 1.0 CB A:ILE94 4.8 15.9 1.0 N A:CYS92 4.8 12.5 1.0 CD1 A:ILE94 4.8 16.0 1.0 O A:HOH351 4.8 22.0 1.0 OD1 A:ASN60 4.9 10.0 1.0 CA A:SER37 4.9 10.4 1.0 CA A:GLY59 5.0 10.5 1.0

Zinc binding site 2 out of 2 in the The Structural Basis of the Beta-Carbonic Anhydrase Cafd (C39A Mutant) of the Filamentous Fungus Aspergillus Fumigatus


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of The Structural Basis of the Beta-Carbonic Anhydrase Cafd (C39A Mutant) of the Filamentous Fungus Aspergillus Fumigatus within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn201 b:10.7 occ:1.00 OD1 B:ASP36 2.0 11.6 1.0 NE2 B:HIS89 2.1 11.1 1.0 SG B:CYS34 2.3 9.8 1.0 SG B:CYS92 2.3 11.2 1.0 CE1 B:HIS89 3.0 11.1 1.0 CG B:ASP36 3.0 11.6 1.0 CD2 B:HIS89 3.1 10.8 1.0 CB B:CYS34 3.1 8.1 1.0 CB B:CYS92 3.3 12.9 1.0 OD2 B:ASP36 3.4 14.6 1.0 CA B:CYS92 3.6 13.4 1.0 ND1 B:HIS89 4.1 11.2 1.0 O B:HOH315 4.1 10.8 1.0 N B:ASP36 4.2 10.2 1.0 CB B:ASP36 4.2 11.3 1.0 CG B:HIS89 4.2 11.1 1.0 C B:ASP36 4.3 11.8 1.0 N B:HIS93 4.3 13.6 1.0 N B:SER37 4.4 11.4 1.0 CA B:ASP36 4.4 11.3 1.0 C B:CYS92 4.5 13.5 1.0 CA B:CYS34 4.5 9.2 1.0 C B:CYS34 4.7 8.2 1.0 O B:ASP36 4.7 12.6 1.0 N B:ILE94 4.8 14.4 1.0 N B:CYS92 4.8 14.4 1.0 O B:HOH358 4.8 26.5 1.0 CD1 B:ILE94 4.8 15.6 1.0 CB B:ILE94 4.8 15.3 1.0 OD1 B:ASN60 4.9 10.0 1.0 CA B:SER37 4.9 11.8 1.0 CA B:GLY59 5.0 10.6 1.0 O B:CYS34 5.0 8.9 1.0

M.S.Jin, S.Kim, N.J.Kim, S.Hong, S.Kim, J.Sung. The Structural Analysis of Non-Catalytic Zinc Binding Site in the Minor Beta-Carbonic Anhydrase Cafd To Be Published.