Zinc in PDB 6jcy: Mycobacterium Tuberculosis Rna Polymerase Transcription Initiation Open Complex with A Chimeric Ecf Sigma Factor Sigh/E

Enzymatic activity of Mycobacterium Tuberculosis Rna Polymerase Transcription Initiation Open Complex with A Chimeric Ecf Sigma Factor Sigh/E

All present enzymatic activity of Mycobacterium Tuberculosis Rna Polymerase Transcription Initiation Open Complex with A Chimeric Ecf Sigma Factor Sigh/E:
2.7.7.6;

Protein crystallography data

The structure of Mycobacterium Tuberculosis Rna Polymerase Transcription Initiation Open Complex with A Chimeric Ecf Sigma Factor Sigh/E, PDB code: 6jcy was solved by L.Li, Y.Zhang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 48.77 / 3.11
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 128.943, 163.005, 129.315, 90.00, 117.81, 90.00
R / Rfree (%) 19.5 / 23.8

Other elements in 6jcy:

The structure of Mycobacterium Tuberculosis Rna Polymerase Transcription Initiation Open Complex with A Chimeric Ecf Sigma Factor Sigh/E also contains other interesting chemical elements:

Magnesium (Mg) 1 atom

Zinc Binding Sites:

The binding sites of Zinc atom in the Mycobacterium Tuberculosis Rna Polymerase Transcription Initiation Open Complex with A Chimeric Ecf Sigma Factor Sigh/E (pdb code 6jcy). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Mycobacterium Tuberculosis Rna Polymerase Transcription Initiation Open Complex with A Chimeric Ecf Sigma Factor Sigh/E, PDB code: 6jcy:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in 6jcy

Go back to Zinc Binding Sites List in 6jcy
Zinc binding site 1 out of 2 in the Mycobacterium Tuberculosis Rna Polymerase Transcription Initiation Open Complex with A Chimeric Ecf Sigma Factor Sigh/E


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Mycobacterium Tuberculosis Rna Polymerase Transcription Initiation Open Complex with A Chimeric Ecf Sigma Factor Sigh/E within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Zn2001

b:99.3
occ:1.00
 SG D:CYS968 2.2 89.6 1.0
SG D:CYS975 2.3 85.9 1.0
SG D:CYS978 2.3 94.2 1.0
SG D:CYS891 2.4 0.2 1.0
CB D:CYS978 2.6 98.2 1.0
CB D:CYS968 3.0 93.9 1.0
CA D:CYS968 3.3 94.2 1.0
CB D:CYS975 3.6 96.1 1.0
NH2 D:ARG963 3.7 94.2 1.0
CB D:CYS891 3.8 0.4 1.0
N D:ALA969 3.9 99.5 1.0
CA D:CYS978 4.0 98.5 1.0
C D:CYS968 4.1 95.9 1.0
OG1 D:THR970 4.1 95.0 1.0
N D:CYS975 4.2 95.0 1.0
N D:CYS978 4.2 99.9 1.0
CA D:CYS975 4.4 97.1 1.0
CG2 D:THR893 4.5 0.3 1.0
N D:CYS968 4.5 90.3 1.0
N D:CYS891 4.5 0.9 1.0
O D:CYS975 4.7 97.0 1.0
N D:THR970 4.7 94.3 1.0
CA D:CYS891 4.8 0.7 1.0
CZ D:ARG963 4.8 95.7 1.0
C D:CYS975 4.9 98.6 1.0

Zinc binding site 2 out of 2 in 6jcy

Go back to Zinc Binding Sites List in 6jcy
Zinc binding site 2 out of 2 in the Mycobacterium Tuberculosis Rna Polymerase Transcription Initiation Open Complex with A Chimeric Ecf Sigma Factor Sigh/E


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Mycobacterium Tuberculosis Rna Polymerase Transcription Initiation Open Complex with A Chimeric Ecf Sigma Factor Sigh/E within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Zn2002

b:0.7
occ:1.00
 SG D:CYS75 2.2 0.5 1.0
SG D:CYS62 2.3 0.1 1.0
SG D:CYS60 2.3 0.2 1.0
SG D:CYS78 2.3 1.0 1.0
CB D:CYS75 3.3 0.7 1.0
CB D:CYS60 3.4 0.1 1.0
N D:CYS62 3.4 0.6 1.0
CB D:CYS62 3.5 0.9 1.0
CB D:CYS78 3.6 0.7 1.0
CA D:CYS62 3.9 0.4 1.0
N D:CYS78 4.1 0.3 1.0
N D:TYR61 4.1 0.9 1.0
C D:CYS62 4.2 0.4 1.0
N D:GLY63 4.2 0.6 1.0
CB D:LYS64 4.2 0.6 1.0
N D:LYS64 4.3 0.2 1.0
CA D:CYS78 4.4 0.1 1.0
CB D:ARG77 4.5 0.9 1.0
C D:CYS60 4.5 0.4 1.0
C D:TYR61 4.5 0.6 1.0
CA D:CYS60 4.6 0.5 1.0
CA D:CYS75 4.7 0.1 1.0
CD2 D:TYR65 4.8 0.3 1.0
CA D:TYR61 4.8 0.3 1.0
CA D:LYS64 4.9 0.9 1.0
C D:ARG77 4.9 0.2 1.0
O D:CYS62 5.0 0.5 1.0

Reference:

C.Fang, L.Li, L.Shen, J.Shi, S.Wang, Y.Feng, Y.Zhang. Structures and Mechanism of Transcription Initiation By Bacterial Ecf Factors. Nucleic Acids Res. V. 47 7094 2019.
ISSN: ESSN 1362-4962
PubMed: 31131408
DOI: 10.1093/NAR/GKZ470
Page generated: Wed Dec 16 12:04:02 2020

Last articles

Zn in 8WB0
Zn in 8WAX
Zn in 8WAU
Zn in 8WAZ
Zn in 8WAY
Zn in 8WAV
Zn in 8WAW
Zn in 8WAT
Zn in 8W7M
Zn in 8WD3
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy