Zinc in PDB 6iv1: Crystal Structure of A Bacterial Bestrophin Homolog From Klebsiella Pneumoniae with A Mutation I180T

The structure of Crystal Structure of A Bacterial Bestrophin Homolog From Klebsiella Pneumoniae with A Mutation I180T, PDB code: 6iv1 was solved by S.Chen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	112.84 / 3.18
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	112.835, 159.247, 159.924, 90.00, 90.00, 90.00
R / Rfree (%)	24.2 / 27.6

Pages:

>>> Page 1 <<< Page 2, Binding sites: 11 - 14;

Binding sites:

The binding sites of Zinc atom in the Crystal Structure of A Bacterial Bestrophin Homolog From Klebsiella Pneumoniae with A Mutation I180T (pdb code 6iv1). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 14 binding sites of Zinc where determined in the Crystal Structure of A Bacterial Bestrophin Homolog From Klebsiella Pneumoniae with A Mutation I180T, PDB code: 6iv1:
Jump to Zinc binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9; 10;

Zinc binding site 1 out of 14 in the Crystal Structure of A Bacterial Bestrophin Homolog From Klebsiella Pneumoniae with A Mutation I180T


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of A Bacterial Bestrophin Homolog From Klebsiella Pneumoniae with A Mutation I180T within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn301 b:82.2 occ:1.00 NE2 E:HIS194 1.9 63.1 1.0 O A:HOH404 2.0 40.5 1.0 OE1 A:GLU191 2.0 0.2 1.0 OE2 A:GLU191 2.1 0.0 1.0 CD A:GLU191 2.3 98.0 1.0 O A:HOH402 2.3 46.9 1.0 CE1 E:HIS194 2.8 64.0 1.0 CD2 E:HIS194 2.9 62.1 1.0 CG A:GLU191 3.8 81.8 1.0 ND1 E:HIS194 3.9 55.7 1.0 CG E:HIS194 4.0 50.0 1.0 CD A:LYS188 4.0 70.4 1.0 CE A:LYS188 4.1 88.1 1.0 CG2 A:ILE91 4.3 63.1 1.0 CB E:ASP190 4.7 77.7 1.0 CB A:GLU191 4.7 69.3 1.0 CG E:ASP190 4.8 94.0 1.0 OD2 E:ASP190 4.8 0.8 1.0 OG1 A:THR95 4.8 79.6 1.0 CA A:ALA92 4.9 54.9 1.0

Zinc binding site 2 out of 14 in the Crystal Structure of A Bacterial Bestrophin Homolog From Klebsiella Pneumoniae with A Mutation I180T


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of A Bacterial Bestrophin Homolog From Klebsiella Pneumoniae with A Mutation I180T within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn302 b:87.2 occ:1.00 OE2 B:GLU191 1.9 0.9 1.0 NE2 A:HIS194 2.0 72.5 1.0 OE1 B:GLU191 2.1 75.2 1.0 O B:HOH401 2.1 42.1 1.0 CD B:GLU191 2.2 79.7 1.0 O B:HOH402 2.7 38.2 1.0 CE1 A:HIS194 2.9 60.8 1.0 CD2 A:HIS194 3.0 68.0 1.0 CG B:GLU191 3.7 68.6 1.0 CE B:LYS188 4.0 82.0 1.0 CD B:LYS188 4.0 79.8 1.0 ND1 A:HIS194 4.0 56.1 1.0 CG A:HIS194 4.1 51.1 1.0 CG2 B:ILE91 4.6 64.0 1.0 CB B:GLU191 4.7 59.6 1.0 NZ B:LYS188 4.8 99.8 1.0 CB A:ASP190 4.9 94.9 1.0 OG1 B:THR95 5.0 77.8 1.0 CA B:ALA92 5.0 50.9 1.0

Zinc binding site 3 out of 14 in the Crystal Structure of A Bacterial Bestrophin Homolog From Klebsiella Pneumoniae with A Mutation I180T


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Crystal Structure of A Bacterial Bestrophin Homolog From Klebsiella Pneumoniae with A Mutation I180T within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn303 b:81.3 occ:1.00 O A:HOH401 2.0 34.4 1.0 O A:HOH403 2.1 37.8 1.0 ND1 A:HIS111 2.1 77.5 1.0 ND1 A:HIS108 2.2 91.7 1.0 CE1 A:HIS108 3.0 93.0 1.0 CE1 A:HIS111 3.0 77.6 1.0 CG A:HIS111 3.1 79.8 1.0 CG A:HIS108 3.3 84.9 1.0 CB A:HIS111 3.4 85.8 1.0 CB A:HIS108 3.7 89.2 1.0 CA A:HIS108 3.8 88.4 1.0 NE2 A:HIS111 4.1 81.6 1.0 NE2 A:HIS108 4.2 94.5 1.0 CD2 A:HIS111 4.2 80.3 1.0 CD2 A:HIS108 4.3 85.6 1.0 CG2 A:THR287 4.4 82.7 1.0 O A:HIS108 4.6 99.8 1.0 CB A:THR287 4.6 92.3 1.0 C A:HIS108 4.7 84.3 1.0 N A:HIS108 4.7 95.4 1.0 CA A:THR287 4.8 0.6 1.0 CG A:ARG101 4.8 83.3 1.0 O A:THR287 4.9 0.7 1.0 CA A:HIS111 4.9 84.4 1.0 NE A:ARG101 4.9 94.4 1.0

Zinc binding site 4 out of 14 in the Crystal Structure of A Bacterial Bestrophin Homolog From Klebsiella Pneumoniae with A Mutation I180T


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Crystal Structure of A Bacterial Bestrophin Homolog From Klebsiella Pneumoniae with A Mutation I180T within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn301 b:0.4 occ:1.00 ND1 B:HIS235 2.2 0.8 1.0 CE1 B:HIS235 3.1 0.5 1.0 CG B:HIS235 3.3 0.7 1.0 CB B:HIS235 3.7 0.1 1.0 CD2 B:TYR42 4.0 0.7 1.0 CA B:HIS235 4.2 0.2 1.0 NE2 B:HIS235 4.2 0.5 1.0 CD2 B:HIS235 4.3 0.5 1.0 CE2 B:TYR42 4.3 0.8 1.0

Zinc binding site 5 out of 14 in the Crystal Structure of A Bacterial Bestrophin Homolog From Klebsiella Pneumoniae with A Mutation I180T


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 5 of Crystal Structure of A Bacterial Bestrophin Homolog From Klebsiella Pneumoniae with A Mutation I180T within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn302 b:91.5 occ:1.00 ND1 B:HIS108 2.0 0.9 1.0 CD2 B:HIS111 2.3 70.8 1.0 NH1 B:ARG101 2.5 0.4 1.0 CG B:HIS108 2.9 0.1 1.0 CE1 B:HIS108 3.0 0.6 1.0 CG B:HIS111 3.2 73.4 1.0 CB B:HIS108 3.3 0.0 1.0 CA B:HIS108 3.4 0.5 1.0 NE2 B:HIS111 3.4 73.8 1.0 CB B:HIS111 3.5 65.8 1.0 CZ B:ARG101 3.8 0.4 1.0 N B:HIS108 4.0 92.4 1.0 NE2 B:HIS108 4.1 0.2 1.0 CD2 B:HIS108 4.1 0.0 1.0 NH2 B:ARG101 4.3 0.9 1.0 ND1 B:HIS111 4.4 97.7 1.0 CE1 B:HIS111 4.4 88.8 1.0 O B:PRO105 4.5 0.3 1.0 C B:HIS108 4.6 0.6 1.0 C B:GLU107 4.7 84.1 1.0 NE B:ARG101 4.7 99.6 1.0 O B:HIS108 4.8 0.9 1.0 CG B:ARG101 4.8 73.2 1.0 O B:GLU107 4.8 85.5 1.0 CD B:ARG101 4.9 85.8 1.0

Zinc binding site 6 out of 14 in the Crystal Structure of A Bacterial Bestrophin Homolog From Klebsiella Pneumoniae with A Mutation I180T


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 6 of Crystal Structure of A Bacterial Bestrophin Homolog From Klebsiella Pneumoniae with A Mutation I180T within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn301 b:72.8 occ:1.00 NE2 D:HIS194 2.0 82.5 1.0 OE1 C:GLU191 2.0 0.2 1.0 OE2 C:GLU191 2.2 97.2 1.0 CD C:GLU191 2.4 91.5 1.0 CD2 D:HIS194 2.8 78.0 1.0 CE1 D:HIS194 3.1 78.6 1.0 NZ C:LYS188 3.6 93.1 1.0 CG C:GLU191 4.0 70.3 1.0 CG D:HIS194 4.0 64.0 1.0 ND1 D:HIS194 4.1 60.3 1.0 CB D:ASP190 4.3 74.3 1.0 CD C:LYS188 4.4 70.9 1.0 CG2 C:ILE91 4.5 67.5 1.0 CE C:LYS188 4.6 80.6 1.0 OG1 C:THR95 4.8 0.8 1.0 CB C:GLU191 4.8 50.7 1.0

Zinc binding site 7 out of 14 in the Crystal Structure of A Bacterial Bestrophin Homolog From Klebsiella Pneumoniae with A Mutation I180T


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 7 of Crystal Structure of A Bacterial Bestrophin Homolog From Klebsiella Pneumoniae with A Mutation I180T within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn302 b:88.0 occ:1.00 NE2 C:HIS194 2.0 68.4 1.0 OE2 E:GLU191 2.1 0.3 1.0 OE1 E:GLU191 2.4 97.8 1.0 CD E:GLU191 2.5 85.4 1.0 CD2 C:HIS194 2.8 59.7 1.0 CE1 C:HIS194 3.1 73.5 1.0 CG E:GLU191 4.0 64.1 1.0 CG C:HIS194 4.0 53.0 1.0 ND1 C:HIS194 4.1 60.3 1.0 CD E:LYS188 4.1 86.8 1.0 CE E:LYS188 4.1 98.3 1.0 CB C:ASP190 4.4 41.7 1.0 CG2 E:ILE91 4.6 52.1 1.0 NZ E:LYS188 4.8 0.3 1.0 OG1 E:THR95 4.9 71.5 1.0 O C:ASP190 4.9 59.0 1.0 CB E:GLU191 5.0 59.9 1.0

Zinc binding site 8 out of 14 in the Crystal Structure of A Bacterial Bestrophin Homolog From Klebsiella Pneumoniae with A Mutation I180T


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 8 of Crystal Structure of A Bacterial Bestrophin Homolog From Klebsiella Pneumoniae with A Mutation I180T within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn303 b:80.1 occ:1.00 ND1 C:HIS111 2.0 78.4 1.0 ND1 C:HIS108 2.2 85.3 1.0 CE1 C:HIS111 2.9 80.4 1.0 CE1 C:HIS108 3.0 83.3 1.0 CG C:HIS111 3.0 80.5 1.0 CG C:HIS108 3.2 92.3 1.0 CB C:HIS111 3.4 84.3 1.0 CB C:HIS108 3.6 0.3 1.0 CA C:HIS108 3.8 95.1 1.0 NE2 C:HIS111 4.1 77.2 1.0 NE2 C:HIS108 4.1 96.2 1.0 CD2 C:HIS111 4.1 75.0 1.0 CD2 C:HIS108 4.2 97.0 1.0 O C:HIS108 4.4 0.6 1.0 NE C:ARG101 4.5 0.1 1.0 C C:HIS108 4.6 97.6 1.0 CG C:ARG101 4.8 98.1 1.0 N C:HIS108 4.8 95.4 1.0 CD C:ARG101 4.8 0.9 1.0 O C:PRO105 4.9 99.7 1.0 CZ C:ARG101 4.9 0.0 1.0 CA C:HIS111 4.9 77.1 1.0

Zinc binding site 9 out of 14 in the Crystal Structure of A Bacterial Bestrophin Homolog From Klebsiella Pneumoniae with A Mutation I180T


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 9 of Crystal Structure of A Bacterial Bestrophin Homolog From Klebsiella Pneumoniae with A Mutation I180T within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn301 b:93.9 occ:1.00 CE1 B:HIS194 1.9 70.2 1.0 OE2 D:GLU191 2.1 0.5 1.0 OE1 D:GLU191 2.3 0.8 1.0 NE2 B:HIS194 2.4 72.2 1.0 CD D:GLU191 2.5 0.3 1.0 NZ D:LYS188 2.8 0.2 1.0 ND1 B:HIS194 3.2 84.6 1.0 CD2 B:HIS194 3.7 48.1 1.0 CE D:LYS188 4.0 96.9 1.0 CG D:GLU191 4.0 80.2 1.0 CD D:LYS188 4.1 90.3 1.0 CG B:HIS194 4.1 54.4 1.0 CB B:ASP190 4.4 83.0 1.0 CG2 D:ILE91 4.8 55.7 1.0 OG1 D:THR95 4.8 79.4 1.0 CB D:GLU191 4.8 61.0 1.0

Zinc binding site 10 out of 14 in the Crystal Structure of A Bacterial Bestrophin Homolog From Klebsiella Pneumoniae with A Mutation I180T


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 10 of Crystal Structure of A Bacterial Bestrophin Homolog From Klebsiella Pneumoniae with A Mutation I180T within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn302 b:0.2 occ:1.00 ND1 D:HIS108 1.9 0.4 1.0 ND1 D:HIS111 2.4 0.3 1.0 CG D:HIS111 2.4 0.3 1.0 CG D:HIS108 2.8 0.2 1.0 CE1 D:HIS108 2.9 0.7 1.0 CE1 D:HIS111 2.9 0.8 1.0 CB D:HIS111 3.0 0.7 1.0 CD2 D:HIS111 3.0 0.1 1.0 CA D:HIS108 3.0 0.8 1.0 CB D:HIS108 3.1 0.6 1.0 NE2 D:HIS111 3.2 1.0 1.0 N D:HIS108 3.9 0.1 1.0 CD2 D:HIS108 3.9 0.3 1.0 NE2 D:HIS108 4.0 0.3 1.0 C D:HIS108 4.0 0.7 1.0 O D:HIS108 4.0 93.4 1.0 CA D:HIS111 4.5 0.5 1.0 C D:GLU107 4.6 0.4 1.0 O D:GLU107 4.9 0.1 1.0 N D:HIS111 4.9 98.7 1.0

C.Ji, A.Kittredge, A.Hopiavuori, N.Ward, S.Chen, Y.Fukuda, Y.Zhang, T.Yang. Dual CA2+-Dependent Gates in Human BESTROPHIN1 Underlie Disease-Causing Mechanisms of Gain-of-Function Mutations. Commun Biol V. 2 240 2019.
ISSN: ESSN 2399-3642
PubMed: 31263784
DOI: 10.1038/S42003-019-0433-3