Zinc in PDB 6hpr: Crystal Structure of CIAP1 Ring Domain Bound to UBCH5B-Ub and A Non- Covalent Ub

All present enzymatic activity of Crystal Structure of CIAP1 Ring Domain Bound to UBCH5B-Ub and A Non- Covalent Ub:
2.3.2.23; 2.3.2.24; 2.3.2.27;

The structure of Crystal Structure of CIAP1 Ring Domain Bound to UBCH5B-Ub and A Non- Covalent Ub, PDB code: 6hpr was solved by A.Patel, D.T.Huang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	23.52 / 1.70
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	79.190, 53.600, 78.540, 90.00, 107.57, 90.00
R / Rfree (%)	17 / 19.7

The binding sites of Zinc atom in the Crystal Structure of CIAP1 Ring Domain Bound to UBCH5B-Ub and A Non- Covalent Ub (pdb code 6hpr). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of CIAP1 Ring Domain Bound to UBCH5B-Ub and A Non- Covalent Ub, PDB code: 6hpr:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of CIAP1 Ring Domain Bound to UBCH5B-Ub and A Non- Covalent Ub


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of CIAP1 Ring Domain Bound to UBCH5B-Ub and A Non- Covalent Ub within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn701 b:22.7 occ:1.00 SG A:CYS571 2.3 16.8 1.0 SG A:CYS595 2.3 22.6 1.0 SG A:CYS592 2.4 23.7 1.0 SG A:CYS574 2.4 25.5 1.0 CB A:CYS571 3.2 17.2 1.0 CB A:CYS574 3.3 26.0 1.0 CB A:CYS595 3.3 20.5 1.0 CB A:CYS592 3.6 20.7 1.0 N A:CYS574 3.6 16.6 1.0 N A:CYS592 3.9 16.4 1.0 CA A:CYS574 4.0 19.0 1.0 N A:CYS595 4.1 26.6 1.0 CA A:CYS592 4.3 16.2 1.0 CA A:CYS595 4.3 24.6 1.0 CA A:CYS571 4.6 17.4 1.0 CB A:VAL573 4.7 14.2 1.0 C A:VAL573 4.8 20.7 1.0 C A:CYS574 4.8 24.6 1.0 N A:MET575 4.8 28.0 1.0 C A:CYS592 4.9 21.2 1.0 O A:CYS592 4.9 21.1 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of CIAP1 Ring Domain Bound to UBCH5B-Ub and A Non- Covalent Ub


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of CIAP1 Ring Domain Bound to UBCH5B-Ub and A Non- Covalent Ub within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn702 b:13.7 occ:1.00 ND1 A:HIS588 2.1 16.5 1.0 SG A:CYS605 2.3 15.1 1.0 SG A:CYS586 2.3 12.1 1.0 SG A:CYS602 2.3 13.2 1.0 CB A:CYS602 3.0 14.5 1.0 CE1 A:HIS588 3.0 19.7 1.0 CG A:HIS588 3.1 15.9 1.0 CB A:CYS586 3.2 16.4 1.0 CB A:HIS588 3.5 14.4 1.0 CB A:CYS605 3.5 15.9 1.0 N A:CYS605 3.9 11.5 1.0 NE2 A:HIS588 4.1 21.0 1.0 CD2 A:HIS588 4.2 21.5 1.0 CA A:CYS605 4.2 15.6 1.0 N A:HIS588 4.4 13.7 1.0 CA A:CYS602 4.5 14.4 1.0 CA A:CYS586 4.5 15.8 1.0 CA A:HIS588 4.6 14.3 1.0 CB A:ILE604 4.7 15.9 1.0 C A:CYS586 4.7 17.9 1.0 CD2 A:PHE583 4.8 15.6 1.0 C A:ILE604 4.8 13.2 1.0 C A:CYS605 4.9 15.6 1.0 N A:GLY607 4.9 16.8 1.0 O A:CYS586 4.9 19.2 1.0

A.Patel, G.J.Sibbet, D.T.Huang. Structural Insights Into Non-Covalent Ubiquitin Activation of the CIAP1-UBCH5B∼Ubiquitin Complex. J. Biol. Chem. V. 294 1240 2019.
ISSN: ESSN 1083-351X
PubMed: 30523153
DOI: 10.1074/JBC.RA118.006045