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Zinc in PDB 6flp: Cryoem Structure of E.Coli Rna Polymerase Paused Elongation Complex Without Rna Hairpin Bound to Nusa

Enzymatic activity of Cryoem Structure of E.Coli Rna Polymerase Paused Elongation Complex Without Rna Hairpin Bound to Nusa

All present enzymatic activity of Cryoem Structure of E.Coli Rna Polymerase Paused Elongation Complex Without Rna Hairpin Bound to Nusa:
2.7.7.6;

Other elements in 6flp:

The structure of Cryoem Structure of E.Coli Rna Polymerase Paused Elongation Complex Without Rna Hairpin Bound to Nusa also contains other interesting chemical elements:

Magnesium (Mg) 1 atom

Zinc Binding Sites:

The binding sites of Zinc atom in the Cryoem Structure of E.Coli Rna Polymerase Paused Elongation Complex Without Rna Hairpin Bound to Nusa (pdb code 6flp). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Cryoem Structure of E.Coli Rna Polymerase Paused Elongation Complex Without Rna Hairpin Bound to Nusa, PDB code: 6flp:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in 6flp

Go back to Zinc Binding Sites List in 6flp
Zinc binding site 1 out of 2 in the Cryoem Structure of E.Coli Rna Polymerase Paused Elongation Complex Without Rna Hairpin Bound to Nusa


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Cryoem Structure of E.Coli Rna Polymerase Paused Elongation Complex Without Rna Hairpin Bound to Nusa within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Zn1502

b:0.5
occ:1.00
N D:CYS72 2.2 0.8 1.0
SG D:CYS72 2.5 0.8 1.0
CB D:CYS72 2.9 0.8 1.0
N D:LEU71 2.9 0.5 1.0
CA D:CYS72 3.1 0.8 1.0
CB D:CYS70 3.1 0.7 1.0
SG D:CYS70 3.2 0.7 1.0
C D:LEU71 3.2 0.5 1.0
CA D:LEU71 3.5 0.5 1.0
C D:CYS70 3.6 0.7 1.0
CB D:LEU71 3.8 0.5 1.0
N D:GLY73 3.9 0.5 1.0
CA D:CYS70 3.9 0.7 1.0
C D:CYS72 3.9 0.8 1.0
CB D:CYS85 3.9 0.7 1.0
SG D:CYS85 4.1 0.7 1.0
O D:LEU71 4.4 0.5 1.0
O D:CYS70 4.4 0.7 1.0
CB D:VAL90 4.4 0.8 1.0
O D:CYS88 4.8 0.0 1.0
CG2 D:VAL90 4.9 0.8 1.0
CG1 D:VAL90 4.9 0.8 1.0
O D:VAL90 5.0 0.8 1.0

Zinc binding site 2 out of 2 in 6flp

Go back to Zinc Binding Sites List in 6flp
Zinc binding site 2 out of 2 in the Cryoem Structure of E.Coli Rna Polymerase Paused Elongation Complex Without Rna Hairpin Bound to Nusa


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Cryoem Structure of E.Coli Rna Polymerase Paused Elongation Complex Without Rna Hairpin Bound to Nusa within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Zn1503

b:0.2
occ:1.00
SG D:CYS888 2.5 0.5 1.0
SG D:CYS895 2.5 0.1 1.0
SG D:CYS814 2.6 0.4 1.0
CB D:CYS898 2.6 0.6 1.0
NH2 D:ARG883 2.8 0.1 1.0
SG D:CYS898 3.2 0.6 1.0
CB D:CYS888 3.5 0.5 1.0
CB D:CYS814 3.6 0.4 1.0
CZ D:ARG883 3.9 0.1 1.0
CA D:CYS888 3.9 0.5 1.0
CA D:CYS898 4.0 0.6 1.0
CB D:CYS895 4.2 0.1 1.0
N D:ASP889 4.2 0.7 1.0
N D:CYS898 4.3 0.6 1.0
NE D:ARG883 4.5 0.1 1.0
N D:CYS814 4.5 0.4 1.0
C D:CYS888 4.7 0.5 1.0
O D:CYS895 4.7 0.1 1.0
CA D:CYS814 4.7 0.4 1.0
N D:CYS895 4.8 0.1 1.0
NH1 D:ARG883 4.9 0.1 1.0
OD1 D:ASP813 4.9 0.6 1.0
CA D:CYS895 5.0 0.1 1.0

Reference:

X.Guo, A.G.Myasnikov, J.Chen, C.Crucifix, G.Papai, M.Takacs, P.Schultz, A.Weixlbaumer. Structural Basis For Nusa Stabilized Transcriptional Pausing. Mol. Cell V. 69 816 2018.
ISSN: ISSN 1097-4164
PubMed: 29499136
DOI: 10.1016/J.MOLCEL.2018.02.008
Page generated: Wed Dec 16 11:48:43 2020

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