Zinc in PDB 6cw2: Crystal Structure of A Yeast Saga Transcriptional Coactivator ADA2/GCN5 Hat Subcomplex, Crystal Form 1

All present enzymatic activity of Crystal Structure of A Yeast Saga Transcriptional Coactivator ADA2/GCN5 Hat Subcomplex, Crystal Form 1:
2.3.1.48;

The structure of Crystal Structure of A Yeast Saga Transcriptional Coactivator ADA2/GCN5 Hat Subcomplex, Crystal Form 1, PDB code: 6cw2 was solved by J.Sun, M.Paduch, S.A.Kim, R.M.Kramer, A.F.Barrios, V.Lu, J.Luke, S.Usatyuk, A.A.Kossiakoff, S.Tan, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	95.91 / 2.67
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	191.816, 191.816, 92.674, 90.00, 90.00, 120.00
R / Rfree (%)	25 / 28.4

The binding sites of Zinc atom in the Crystal Structure of A Yeast Saga Transcriptional Coactivator ADA2/GCN5 Hat Subcomplex, Crystal Form 1 (pdb code 6cw2). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of A Yeast Saga Transcriptional Coactivator ADA2/GCN5 Hat Subcomplex, Crystal Form 1, PDB code: 6cw2:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of A Yeast Saga Transcriptional Coactivator ADA2/GCN5 Hat Subcomplex, Crystal Form 1


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of A Yeast Saga Transcriptional Coactivator ADA2/GCN5 Hat Subcomplex, Crystal Form 1 within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn201 b:91.7 occ:1.00 ND1 C:HIS48 2.1 0.3 1.0 ND1 C:HIS44 2.1 83.5 1.0 SG C:CYS22 2.2 84.3 1.0 SG C:CYS25 2.3 88.6 1.0 CE1 C:HIS48 2.9 94.3 1.0 CE1 C:HIS44 2.9 74.2 1.0 CB C:CYS25 3.1 88.3 1.0 CG C:HIS48 3.1 0.0 1.0 CG C:HIS44 3.2 93.1 1.0 CB C:CYS22 3.3 67.1 1.0 CB C:HIS48 3.6 94.1 1.0 CB C:HIS44 3.6 89.7 1.0 CA C:HIS44 3.6 91.9 1.0 N C:CYS25 3.8 90.4 1.0 N C:ARG45 4.0 73.9 1.0 NE2 C:HIS48 4.0 96.2 1.0 CA C:CYS25 4.0 97.9 1.0 NE2 C:HIS44 4.1 69.2 1.0 CD2 C:HIS48 4.2 98.4 1.0 CD2 C:HIS44 4.2 69.5 1.0 C C:HIS44 4.4 73.6 1.0 O C:ARG45 4.4 82.0 1.0 CB C:ILE24 4.5 87.0 1.0 C C:ILE24 4.6 94.1 1.0 CA C:CYS22 4.7 80.5 1.0 N C:HIS44 4.7 86.6 1.0 CA C:ILE24 4.9 94.5 1.0 N C:ILE24 4.9 92.1 1.0 CD1 C:TYR28 5.0 73.0 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of A Yeast Saga Transcriptional Coactivator ADA2/GCN5 Hat Subcomplex, Crystal Form 1


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of A Yeast Saga Transcriptional Coactivator ADA2/GCN5 Hat Subcomplex, Crystal Form 1 within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn202 b:91.3 occ:1.00 SG C:CYS10 2.2 67.1 1.0 SG C:CYS31 2.3 72.0 1.0 SG C:CYS34 2.4 83.3 1.0 SG C:CYS7 2.5 92.2 1.0 CB C:CYS10 3.0 70.0 1.0 CB C:CYS34 3.1 79.9 1.0 CB C:CYS31 3.4 57.9 1.0 N C:CYS10 3.7 95.4 1.0 CB C:CYS7 3.7 66.9 1.0 CA C:CYS10 3.9 82.5 1.0 N C:CYS31 4.0 75.7 1.0 N C:CYS34 4.2 73.5 1.0 CA C:CYS34 4.2 73.3 1.0 CA C:CYS31 4.2 61.0 1.0 CB C:VAL9 4.6 90.3 1.0 C C:CYS10 4.6 83.4 1.0 O C:CYS31 4.7 73.8 1.0 C C:CYS31 4.7 69.8 1.0 CB C:ALA12 4.8 72.2 1.0 C C:VAL9 4.8 84.5 1.0 N C:SER11 4.9 87.1 1.0

J.Sun, M.Paduch, S.A.Kim, R.M.Kramer, A.F.Barrios, V.Lu, J.Luke, S.Usatyuk, A.A.Kossiakoff, S.Tan. Structural Basis For Activation of Saga Histone Acetyltransferase GCN5 By Partner Subunit ADA2. Proc. Natl. Acad. Sci. V. 115 10010 2018U.S.A..
ISSN: ESSN 1091-6490
PubMed: 30224453
DOI: 10.1073/PNAS.1805343115