Zinc in PDB 6cuu: Thermus Thermophiles Rna Polymerase in Complex with Promoter Dna and Antibiotic Kanglemycin A

All present enzymatic activity of Thermus Thermophiles Rna Polymerase in Complex with Promoter Dna and Antibiotic Kanglemycin A:
2.7.7.6;

The structure of Thermus Thermophiles Rna Polymerase in Complex with Promoter Dna and Antibiotic Kanglemycin A, PDB code: 6cuu was solved by V.Molodtsov, K.S.Murakami, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	46.31 / 2.99
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	185.221, 101.315, 294.279, 90.00, 98.89, 90.00
R / Rfree (%)	20.1 / 24.8

The structure of Thermus Thermophiles Rna Polymerase in Complex with Promoter Dna and Antibiotic Kanglemycin A also contains other interesting chemical elements:

Magnesium

(Mg)

2 atoms

The binding sites of Zinc atom in the Thermus Thermophiles Rna Polymerase in Complex with Promoter Dna and Antibiotic Kanglemycin A (pdb code 6cuu). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Thermus Thermophiles Rna Polymerase in Complex with Promoter Dna and Antibiotic Kanglemycin A, PDB code: 6cuu:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Thermus Thermophiles Rna Polymerase in Complex with Promoter Dna and Antibiotic Kanglemycin A


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Thermus Thermophiles Rna Polymerase in Complex with Promoter Dna and Antibiotic Kanglemycin A within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn2001 b:65.2 occ:1.00 SG D:CYS1201 2.1 76.2 1.0 SG D:CYS1112 2.2 59.5 1.0 SG D:CYS1194 2.3 64.1 1.0 SG D:CYS1204 2.5 55.4 1.0 CB D:CYS1204 3.2 50.3 1.0 CB D:CYS1194 3.3 46.3 1.0 CB D:CYS1112 3.4 49.9 1.0 CB D:CYS1201 3.5 71.6 1.0 CA D:CYS1194 3.6 43.7 1.0 OG1 D:THR1196 3.7 65.6 1.0 N D:CYS1201 4.1 73.3 1.0 N D:CYS1112 4.1 48.7 1.0 N D:GLN1195 4.1 50.0 1.0 N D:CYS1204 4.2 51.6 1.0 CG2 D:THR1114 4.3 74.8 1.0 CA D:CYS1201 4.3 72.8 1.0 CA D:CYS1204 4.3 52.9 1.0 NH2 D:ARG1189 4.3 61.5 1.0 C D:CYS1194 4.4 54.2 1.0 CA D:CYS1112 4.4 43.7 1.0 N D:THR1196 4.6 45.3 1.0 N D:CYS1194 4.8 36.5 1.0 CB D:THR1196 4.9 45.1 1.0 O D:CYS1201 4.9 64.8 1.0 C D:CYS1201 4.9 67.3 1.0

Zinc binding site 2 out of 2 in the Thermus Thermophiles Rna Polymerase in Complex with Promoter Dna and Antibiotic Kanglemycin A


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Thermus Thermophiles Rna Polymerase in Complex with Promoter Dna and Antibiotic Kanglemycin A within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn2002 b:91.6 occ:1.00 SG D:CYS58 2.1 0.6 1.0 SG D:CYS60 2.1 0.9 1.0 SG D:CYS73 2.2 95.2 1.0 SG D:CYS76 2.5 96.0 1.0 CB D:CYS73 3.1 88.2 1.0 CB D:CYS58 3.2 97.7 1.0 N D:LYS62 3.5 0.0 1.0 CB D:CYS76 3.6 98.4 1.0 N D:GLY61 3.6 0.2 1.0 CB D:CYS60 3.6 0.9 1.0 N D:CYS60 3.6 0.9 1.0 CB D:LYS62 3.8 0.5 1.0 CA D:CYS60 4.0 0.3 1.0 C D:CYS60 4.1 0.7 1.0 N D:CYS76 4.1 0.3 1.0 CA D:LYS62 4.2 0.3 1.0 N D:ALA59 4.2 79.1 1.0 C D:CYS58 4.3 77.1 1.0 CA D:CYS58 4.3 91.6 1.0 C D:GLY61 4.4 0.7 1.0 CA D:CYS76 4.5 0.3 1.0 CA D:GLY61 4.5 0.6 1.0 CA D:CYS73 4.6 96.9 1.0 N D:TYR63 4.6 0.8 1.0 C D:ALA59 4.7 93.3 1.0 CD2 D:TYR63 4.8 99.4 1.0 O D:CYS58 4.9 67.0 1.0 C D:LYS62 5.0 1.0 1.0 CB D:ARG75 5.0 0.5 1.0

H.Mosaei, V.Molodtsov, B.Kepplinger, J.Harbottle, C.W.Moon, R.E.Jeeves, L.Ceccaroni, Y.Shin, S.Morton-Laing, E.C.L.Marrs, C.Wills, W.Clegg, Y.Yuzenkova, J.D.Perry, J.Bacon, J.Errington, N.E.E.Allenby, M.J.Hall, K.S.Murakami, N.Zenkin. Mode of Action of Kanglemycin A, An Ansamycin Natural Product That Is Active Against Rifampicin-Resistant Mycobacterium Tuberculosis. Mol. Cell V. 72 263 2018.
ISSN: ISSN 1097-4164
PubMed: 30244835
DOI: 10.1016/J.MOLCEL.2018.08.028