Zinc in PDB 6alf: Cryoem Structure of Crosslinked E.Coli Rna Polymerase Elongation Complex

All present enzymatic activity of Cryoem Structure of Crosslinked E.Coli Rna Polymerase Elongation Complex:
2.7.7.6;

The structure of Cryoem Structure of Crosslinked E.Coli Rna Polymerase Elongation Complex also contains other interesting chemical elements:

Magnesium

(Mg)

1 atom

The binding sites of Zinc atom in the Cryoem Structure of Crosslinked E.Coli Rna Polymerase Elongation Complex (pdb code 6alf). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Cryoem Structure of Crosslinked E.Coli Rna Polymerase Elongation Complex, PDB code: 6alf:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Cryoem Structure of Crosslinked E.Coli Rna Polymerase Elongation Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Cryoem Structure of Crosslinked E.Coli Rna Polymerase Elongation Complex within 5.0Å range: probe atom residue distance (Å) B Occ J:Zn1502 b:1.0 occ:1.00 N J:CYS88 2.3 0.8 1.0 SG J:CYS72 2.5 0.2 1.0 SG J:CYS88 2.5 0.8 1.0 CB J:CYS72 2.9 0.2 1.0 SG J:CYS85 2.9 0.3 1.0 CB J:LYS87 3.0 0.2 1.0 C J:LYS87 3.1 0.2 1.0 CA J:CYS88 3.2 0.8 1.0 CB J:CYS88 3.2 0.8 1.0 SG J:CYS70 3.2 0.8 1.0 CA J:LYS87 3.4 0.2 1.0 N J:LYS87 3.5 0.2 1.0 N J:GLY89 3.8 0.9 1.0 C J:CYS88 3.8 0.8 1.0 CB J:CYS85 3.8 0.3 1.0 N J:CYS72 3.9 0.2 1.0 CA J:CYS72 4.0 0.2 1.0 O J:LYS87 4.1 0.2 1.0 CG J:LYS87 4.2 0.2 1.0 CB J:CYS70 4.6 0.8 1.0 C J:GLU86 4.6 0.3 1.0 CD J:LYS87 4.6 0.2 1.0 O J:CYS88 4.8 0.8 1.0 N J:GLU86 4.9 0.3 1.0 C J:CYS72 4.9 0.2 1.0

Zinc binding site 2 out of 2 in the Cryoem Structure of Crosslinked E.Coli Rna Polymerase Elongation Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Cryoem Structure of Crosslinked E.Coli Rna Polymerase Elongation Complex within 5.0Å range: probe atom residue distance (Å) B Occ J:Zn1503 b:1.0 occ:1.00 SG J:CYS888 2.4 0.3 1.0 SG J:CYS895 2.4 0.0 1.0 SG J:CYS898 2.5 0.0 1.0 CB J:CYS814 2.5 0.1 1.0 SG J:CYS814 2.5 0.1 1.0 CB J:CYS898 3.4 0.0 1.0 CB J:CYS888 3.5 0.3 1.0 N J:CYS814 3.5 0.1 1.0 CA J:CYS814 3.6 0.1 1.0 CB J:CYS895 3.6 0.0 1.0 OG1 J:THR890 3.8 0.5 1.0 N J:ASP889 3.8 0.4 1.0 CA J:CYS888 3.8 0.3 1.0 C J:CYS888 4.2 0.3 1.0 NH2 J:ARG883 4.2 0.1 1.0 N J:CYS898 4.5 0.0 1.0 N J:CYS895 4.5 0.0 1.0 CA J:CYS898 4.5 0.0 1.0 CA J:CYS895 4.6 0.0 1.0 C J:ASP813 4.6 0.1 1.0 N J:THR890 4.7 0.5 1.0 C J:CYS814 4.7 0.1 1.0 OD1 J:ASP813 4.8 0.1 1.0 CA J:ASP889 4.8 0.4 1.0 CZ J:ARG883 4.8 0.1 1.0 OG1 J:THR816 4.9 0.1 1.0 C J:ASP889 4.9 0.4 1.0 NE J:ARG883 5.0 0.1 1.0

J.Y.Kang, P.D.Olinares, J.Chen, E.A.Campbell, A.Mustaev, B.T.Chait, M.E.Gottesman, S.A.Darst. Structural Basis of Transcription Arrest By Coliphage HK022 Nun in Anescherichia Colirna Polymerase Elongation Complex. Elife V. 6 2017.
ISSN: ESSN 2050-084X
PubMed: 28318486
DOI: 10.7554/ELIFE.25478