Zinc in PDB 5yno: Crystal Structure of Mers-Cov NSP16/NSP10 Complex Bound to Sah and M7GPPPA

The structure of Crystal Structure of Mers-Cov NSP16/NSP10 Complex Bound to Sah and M7GPPPA, PDB code: 5yno was solved by S.M.Wei, L.Yang, Z.H.Ke, D.Y.Guo, C.P.Fan, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	48.54 / 1.96
Space group	P 21 2 21
Cell size a, b, c (Å), α, β, γ (°)	67.193, 70.186, 119.353, 90.00, 90.00, 90.00
R / Rfree (%)	19.3 / 22.7

The binding sites of Zinc atom in the Crystal Structure of Mers-Cov NSP16/NSP10 Complex Bound to Sah and M7GPPPA (pdb code 5yno). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of Mers-Cov NSP16/NSP10 Complex Bound to Sah and M7GPPPA, PDB code: 5yno:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of Mers-Cov NSP16/NSP10 Complex Bound to Sah and M7GPPPA


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Mers-Cov NSP16/NSP10 Complex Bound to Sah and M7GPPPA within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn201 b:46.9 occ:1.00 NE2 B:HIS83 2.0 46.4 1.0 SG B:CYS90 2.3 50.9 1.0 SG B:CYS77 2.3 47.2 1.0 SG B:CYS74 2.3 46.1 1.0 CD2 B:HIS83 2.9 48.9 1.0 CE1 B:HIS83 3.1 46.6 1.0 CB B:CYS74 3.2 45.6 1.0 CB B:CYS90 3.2 50.0 1.0 CB B:CYS77 3.4 42.7 1.0 N B:CYS77 3.6 46.5 1.0 CA B:CYS77 3.9 44.7 1.0 CG B:HIS83 4.1 49.5 1.0 ND1 B:HIS83 4.2 47.4 1.0 CA B:CYS90 4.4 53.7 1.0 CB B:TYR76 4.5 48.5 1.0 C B:TYR76 4.5 50.2 1.0 CA B:CYS74 4.6 45.2 1.0 O B:HOH322 4.6 48.8 1.0 CB B:LYS93 4.7 43.3 1.0 O B:HOH321 4.8 57.9 1.0 CA B:TYR76 4.9 49.4 1.0 N B:TYR76 5.0 50.4 1.0 N B:LYS93 5.0 42.0 1.0 C B:CYS74 5.0 45.2 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of Mers-Cov NSP16/NSP10 Complex Bound to Sah and M7GPPPA


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Mers-Cov NSP16/NSP10 Complex Bound to Sah and M7GPPPA within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn202 b:81.5 occ:1.00 SG B:CYS120 2.3 77.9 1.0 SG B:CYS117 2.3 82.4 1.0 SG B:CYS130 2.4 86.8 1.0 SG B:CYS128 2.4 80.1 1.0 CB B:CYS120 2.9 81.0 1.0 CB B:CYS117 3.0 79.3 1.0 CB B:CYS130 3.2 89.4 1.0 CB B:CYS128 3.5 76.8 1.0 N B:CYS120 3.8 82.9 1.0 N B:CYS130 3.8 90.3 1.0 CA B:CYS120 3.9 81.7 1.0 CA B:CYS128 4.0 77.9 1.0 CG1 B:VAL119 4.1 93.1 1.0 CA B:CYS130 4.1 91.8 1.0 N B:ASN129 4.2 83.2 1.0 C B:CYS128 4.4 81.1 1.0 CA B:CYS117 4.5 79.5 1.0 C B:CYS120 4.7 79.9 1.0 C B:ASN129 4.8 92.7 1.0 C B:VAL119 4.9 86.3 1.0 N B:GLN121 5.0 80.6 1.0

S.M.Wei, L.Yang, Z.H.Ke, Q.Y.Liu, Y.Chen, D.Y.Guo, C.P.Fan. Structural Insights Into the Molecular Mechanism of Mers Coronavirus Rna Ribose 2'-O-Methylation By NSP16/NSP10 Protein Complex To Be Published.