Zinc in PDB 5xt5: Sufs-Sufu Complex From Bacillus Subtilis

All present enzymatic activity of Sufs-Sufu Complex From Bacillus Subtilis:
2.8.1.7;

The structure of Sufs-Sufu Complex From Bacillus Subtilis, PDB code: 5xt5 was solved by T.Fujishiro, Y.Takahashi, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	64.93 / 2.34
Space group	P 65
Cell size a, b, c (Å), α, β, γ (°)	74.980, 74.980, 364.410, 90.00, 90.00, 120.00
R / Rfree (%)	19.9 / 24.3

The binding sites of Zinc atom in the Sufs-Sufu Complex From Bacillus Subtilis (pdb code 5xt5). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Sufs-Sufu Complex From Bacillus Subtilis, PDB code: 5xt5:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Sufs-Sufu Complex From Bacillus Subtilis


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Sufs-Sufu Complex From Bacillus Subtilis within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn201 b:73.9 occ:1.00 ND1 B:HIS342 2.0 94.1 1.0 OD2 D:ASP43 2.0 0.2 1.0 SG D:CYS66 2.3 0.2 1.0 SG D:CYS128 2.4 90.4 1.0 OD1 D:ASP43 2.7 98.5 1.0 CG D:ASP43 2.8 97.3 1.0 CE1 B:HIS342 2.9 89.7 1.0 CG B:HIS342 3.0 89.2 1.0 CB D:CYS66 3.3 0.3 1.0 CB B:HIS342 3.4 82.9 1.0 CB D:CYS128 3.5 91.2 1.0 NE2 B:HIS342 4.0 84.1 1.0 ND2 D:ASN38 4.1 96.1 1.0 CD2 B:HIS342 4.1 85.8 1.0 OG D:SER69 4.2 95.4 1.0 CB D:ASP43 4.2 93.9 1.0 N D:CYS66 4.2 1.0 1.0 NE D:ARG125 4.2 78.1 1.0 CA D:CYS128 4.4 83.9 1.0 CA D:CYS66 4.4 0.3 1.0 CE1 B:HIS340 4.4 88.6 1.0 ND1 B:HIS340 4.4 88.3 1.0 NH1 D:ARG125 4.5 88.5 1.0 CB D:SER69 4.5 96.9 1.0 CZ D:ARG125 4.7 83.4 1.0 OG1 D:THR40 4.9 86.8 1.0 CA B:HIS342 4.9 78.5 1.0

Zinc binding site 2 out of 2 in the Sufs-Sufu Complex From Bacillus Subtilis


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Sufs-Sufu Complex From Bacillus Subtilis within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn201 b:64.1 occ:1.00 OD1 C:ASP43 2.0 0.5 1.0 ND1 A:HIS342 2.1 69.3 1.0 SG C:CYS66 2.3 87.3 1.0 SG C:CYS128 2.4 66.8 1.0 CG C:ASP43 2.7 0.3 1.0 OD2 C:ASP43 2.8 0.3 1.0 CE1 A:HIS342 2.9 66.8 1.0 CG A:HIS342 3.2 64.7 1.0 CB C:CYS128 3.3 65.2 1.0 CB C:CYS66 3.5 87.6 1.0 CB A:HIS342 3.7 61.3 1.0 ND2 C:ASN38 4.0 0.9 1.0 NE2 A:HIS342 4.1 63.6 1.0 CA C:CYS128 4.1 63.2 1.0 CB C:ASP43 4.2 0.3 1.0 CD2 A:HIS342 4.2 63.0 1.0 NE C:ARG125 4.3 55.3 1.0 OG C:SER69 4.3 78.4 1.0 N C:CYS66 4.4 85.1 1.0 CB C:SER69 4.5 76.5 1.0 CA C:CYS66 4.6 84.0 1.0 NH2 C:ARG125 4.7 57.7 1.0 CE1 A:HIS340 4.8 53.1 1.0 CZ C:ARG125 4.9 56.1 1.0 N C:CYS128 5.0 63.1 1.0

T.Fujishiro, T.Terahata, K.Kunichika, N.Yokoyama, C.Maruyama, K.Asai, Y.Takahashi. Zinc-Ligand Swapping Mediated Complex Formation and Sulfur Transfer Between Sufs and Sufu For Iron-Sulfur Cluster Biogenesis in Bacillus Subtilis J. Am. Chem. Soc. V. 139 18464 2017.
ISSN: ESSN 1520-5126
PubMed: 29235855
DOI: 10.1021/JACS.7B11307