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Zinc in PDB 5wb2: US28 Bound to Engineered Chemokine CX3CL1.35 and Nanobodies

Protein crystallography data

The structure of US28 Bound to Engineered Chemokine CX3CL1.35 and Nanobodies, PDB code: 5wb2 was solved by K.M.Jude, J.S.Burg, N.Tsutsumi, T.F.Miles, K.C.Garcia, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 45.37 / 3.50
Space group P 21 21 2
Cell size a, b, c (Å), α, β, γ (°) 47.170, 128.940, 127.740, 90.00, 90.00, 90.00
R / Rfree (%) 25.2 / 29.6

Zinc Binding Sites:

The binding sites of Zinc atom in the US28 Bound to Engineered Chemokine CX3CL1.35 and Nanobodies (pdb code 5wb2). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the US28 Bound to Engineered Chemokine CX3CL1.35 and Nanobodies, PDB code: 5wb2:

Zinc binding site 1 out of 1 in 5wb2

Go back to Zinc Binding Sites List in 5wb2
Zinc binding site 1 out of 1 in the US28 Bound to Engineered Chemokine CX3CL1.35 and Nanobodies


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of US28 Bound to Engineered Chemokine CX3CL1.35 and Nanobodies within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn101

b:0.5
occ:1.00
NE2 B:HIS64 2.4 0.6 1.0
CE1 B:HIS64 3.2 0.1 1.0
CD2 B:HIS64 3.5 0.6 1.0
O B:VAL21 3.7 0.5 1.0
NH2 B:ARG44 4.1 95.7 1.0
O B:LEU24 4.2 89.0 1.0
ND1 B:HIS64 4.4 0.5 1.0
CA B:ALA22 4.4 0.1 1.0
CG B:HIS64 4.6 0.6 1.0
C B:VAL21 4.7 0.8 1.0
O B:ALA22 4.8 0.8 1.0
C B:ALA22 4.8 0.5 1.0

Reference:

T.F.Miles, K.Spiess, K.M.Jude, N.Tsutsumi, J.S.Burg, J.R.Ingram, D.Waghray, G.M.Hjorto, O.Larsen, H.L.Ploegh, M.M.Rosenkilde, K.C.Garcia. Viral Gpcr US28 Can Signal in Response to Chemokine Agonists of Nearly Unlimited Structural Degeneracy. Elife V. 7 2018.
ISSN: ESSN 2050-084X
PubMed: 29882741
DOI: 10.7554/ELIFE.35850
Page generated: Mon Oct 28 14:02:24 2024

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