Zinc in PDB 5w1t: X-Ray Crystal Structure of Escherichia Coli Rna Polymerase and Dksa Complex
Enzymatic activity of X-Ray Crystal Structure of Escherichia Coli Rna Polymerase and Dksa Complex
All present enzymatic activity of X-Ray Crystal Structure of Escherichia Coli Rna Polymerase and Dksa Complex:
2.7.7.6;
Protein crystallography data
The structure of X-Ray Crystal Structure of Escherichia Coli Rna Polymerase and Dksa Complex, PDB code: 5w1t
was solved by
K.S.Murakami,
V.Molodtsov,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
47.08 /
4.50
|
Space group
|
P 21 21 21
|
Cell size a, b, c (Å), α, β, γ (°)
|
184.000,
204.894,
314.312,
90.00,
90.00,
90.00
|
R / Rfree (%)
|
22 /
27.6
|
Other elements in 5w1t:
The structure of X-Ray Crystal Structure of Escherichia Coli Rna Polymerase and Dksa Complex also contains other interesting chemical elements:
Zinc Binding Sites:
The binding sites of Zinc atom in the X-Ray Crystal Structure of Escherichia Coli Rna Polymerase and Dksa Complex
(pdb code 5w1t). This binding sites where shown within
5.0 Angstroms radius around Zinc atom.
In total 6 binding sites of Zinc where determined in the
X-Ray Crystal Structure of Escherichia Coli Rna Polymerase and Dksa Complex, PDB code: 5w1t:
Jump to Zinc binding site number:
1;
2;
3;
4;
5;
6;
Zinc binding site 1 out
of 6 in 5w1t
Go back to
Zinc Binding Sites List in 5w1t
Zinc binding site 1 out
of 6 in the X-Ray Crystal Structure of Escherichia Coli Rna Polymerase and Dksa Complex
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 1 of X-Ray Crystal Structure of Escherichia Coli Rna Polymerase and Dksa Complex within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:Zn2002
b:0.2
occ:1.00
|
SG
|
D:CYS72
|
2.1
|
0.4
|
1.0
|
SG
|
D:CYS85
|
2.3
|
0.8
|
1.0
|
SG
|
D:CYS70
|
2.3
|
0.1
|
1.0
|
SG
|
D:CYS88
|
2.4
|
1.0
|
1.0
|
CB
|
D:CYS85
|
2.8
|
0.5
|
1.0
|
CB
|
D:CYS72
|
2.8
|
0.6
|
1.0
|
N
|
D:CYS72
|
3.4
|
0.4
|
1.0
|
O
|
D:CYS88
|
3.6
|
0.1
|
1.0
|
N
|
D:CYS88
|
3.7
|
0.9
|
1.0
|
CA
|
D:CYS72
|
3.7
|
0.6
|
1.0
|
CB
|
D:CYS88
|
4.0
|
0.2
|
1.0
|
CB
|
D:CYS70
|
4.1
|
0.8
|
1.0
|
CB
|
D:LYS87
|
4.2
|
0.6
|
1.0
|
CA
|
D:CYS88
|
4.2
|
0.6
|
1.0
|
CA
|
D:CYS85
|
4.3
|
0.6
|
1.0
|
N
|
D:GLY73
|
4.3
|
0.7
|
1.0
|
C
|
D:CYS88
|
4.4
|
0.2
|
1.0
|
N
|
D:LEU71
|
4.4
|
0.2
|
1.0
|
C
|
D:CYS72
|
4.5
|
1.0
|
1.0
|
C
|
D:LEU71
|
4.6
|
0.8
|
1.0
|
C
|
D:CYS85
|
4.7
|
0.2
|
1.0
|
N
|
D:LYS74
|
4.8
|
0.5
|
1.0
|
C
|
D:LYS87
|
4.8
|
0.4
|
1.0
|
CA
|
D:LYS87
|
4.9
|
0.8
|
1.0
|
C
|
D:CYS70
|
4.9
|
0.1
|
1.0
|
CA
|
D:LEU71
|
4.9
|
0.2
|
1.0
|
CB
|
D:LYS74
|
4.9
|
0.3
|
1.0
|
CB
|
D:VAL90
|
4.9
|
0.4
|
1.0
|
N
|
D:LYS87
|
4.9
|
1.0
|
1.0
|
O
|
D:CYS85
|
4.9
|
0.7
|
1.0
|
CG2
|
D:VAL90
|
5.0
|
0.6
|
1.0
|
|
Zinc binding site 2 out
of 6 in 5w1t
Go back to
Zinc Binding Sites List in 5w1t
Zinc binding site 2 out
of 6 in the X-Ray Crystal Structure of Escherichia Coli Rna Polymerase and Dksa Complex
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 2 of X-Ray Crystal Structure of Escherichia Coli Rna Polymerase and Dksa Complex within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:Zn2003
b:0.1
occ:1.00
|
SG
|
D:CYS898
|
2.0
|
0.5
|
1.0
|
SG
|
D:CYS888
|
2.1
|
0.4
|
1.0
|
SG
|
D:CYS895
|
2.3
|
0.6
|
1.0
|
SG
|
D:CYS814
|
2.3
|
0.5
|
1.0
|
CB
|
D:CYS888
|
2.6
|
0.1
|
1.0
|
CB
|
D:CYS898
|
2.9
|
0.1
|
1.0
|
CA
|
D:CYS888
|
3.1
|
0.3
|
1.0
|
CB
|
D:CYS895
|
3.6
|
0.2
|
1.0
|
CB
|
D:CYS814
|
3.6
|
0.0
|
1.0
|
C
|
D:CYS888
|
3.9
|
0.0
|
1.0
|
N
|
D:ASP889
|
4.0
|
0.9
|
1.0
|
CA
|
D:CYS898
|
4.1
|
0.6
|
1.0
|
NH2
|
D:ARG883
|
4.1
|
0.6
|
1.0
|
N
|
D:CYS898
|
4.1
|
0.9
|
1.0
|
N
|
D:CYS895
|
4.3
|
0.8
|
1.0
|
N
|
D:CYS888
|
4.3
|
0.8
|
1.0
|
CA
|
D:CYS895
|
4.4
|
0.7
|
1.0
|
N
|
D:CYS814
|
4.5
|
0.6
|
1.0
|
O
|
D:CYS895
|
4.6
|
0.2
|
1.0
|
CA
|
D:CYS814
|
4.7
|
0.0
|
1.0
|
O
|
D:CYS888
|
4.8
|
0.8
|
1.0
|
CG2
|
D:THR816
|
4.8
|
0.7
|
1.0
|
N
|
D:THR890
|
4.9
|
0.9
|
1.0
|
C
|
D:CYS895
|
4.9
|
0.8
|
1.0
|
NE
|
D:ARG883
|
5.0
|
0.3
|
1.0
|
|
Zinc binding site 3 out
of 6 in 5w1t
Go back to
Zinc Binding Sites List in 5w1t
Zinc binding site 3 out
of 6 in the X-Ray Crystal Structure of Escherichia Coli Rna Polymerase and Dksa Complex
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 3 of X-Ray Crystal Structure of Escherichia Coli Rna Polymerase and Dksa Complex within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
J:Zn2002
b:0.8
occ:1.00
|
SG
|
J:CYS70
|
2.0
|
0.3
|
1.0
|
SG
|
J:CYS72
|
2.3
|
0.1
|
1.0
|
SG
|
J:CYS85
|
2.3
|
0.1
|
1.0
|
SG
|
J:CYS88
|
2.6
|
0.4
|
1.0
|
CB
|
J:CYS85
|
2.8
|
0.8
|
1.0
|
CB
|
J:CYS72
|
3.2
|
0.5
|
1.0
|
CB
|
J:CYS70
|
3.7
|
0.0
|
1.0
|
O
|
J:CYS88
|
3.7
|
0.5
|
1.0
|
N
|
J:CYS72
|
3.8
|
1.0
|
1.0
|
N
|
J:CYS88
|
4.0
|
0.1
|
1.0
|
CA
|
J:CYS72
|
4.1
|
0.2
|
1.0
|
CB
|
J:CYS88
|
4.3
|
0.7
|
1.0
|
CA
|
J:CYS85
|
4.3
|
0.5
|
1.0
|
N
|
J:GLY73
|
4.3
|
0.4
|
1.0
|
CB
|
J:LYS87
|
4.4
|
0.4
|
1.0
|
N
|
J:LEU71
|
4.5
|
0.3
|
1.0
|
CA
|
J:CYS88
|
4.5
|
0.9
|
1.0
|
C
|
J:CYS88
|
4.6
|
0.8
|
1.0
|
C
|
J:CYS72
|
4.6
|
0.3
|
1.0
|
N
|
J:LYS74
|
4.6
|
0.4
|
1.0
|
CA
|
J:CYS70
|
4.7
|
0.3
|
1.0
|
CB
|
J:LYS74
|
4.7
|
0.9
|
1.0
|
C
|
J:CYS70
|
4.8
|
0.8
|
1.0
|
C
|
J:CYS85
|
4.8
|
0.4
|
1.0
|
C
|
J:LEU71
|
4.9
|
0.5
|
1.0
|
CB
|
J:VAL90
|
5.0
|
1.0
|
1.0
|
|
Zinc binding site 4 out
of 6 in 5w1t
Go back to
Zinc Binding Sites List in 5w1t
Zinc binding site 4 out
of 6 in the X-Ray Crystal Structure of Escherichia Coli Rna Polymerase and Dksa Complex
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 4 of X-Ray Crystal Structure of Escherichia Coli Rna Polymerase and Dksa Complex within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
J:Zn2003
b:0.8
occ:1.00
|
SG
|
J:CYS888
|
2.3
|
0.1
|
1.0
|
SG
|
J:CYS895
|
2.3
|
0.7
|
1.0
|
SG
|
J:CYS814
|
2.4
|
0.9
|
1.0
|
CB
|
J:CYS888
|
2.6
|
0.2
|
1.0
|
SG
|
J:CYS898
|
2.8
|
0.7
|
1.0
|
CB
|
J:CYS898
|
2.8
|
0.6
|
1.0
|
CA
|
J:CYS888
|
3.2
|
1.0
|
1.0
|
NH2
|
J:ARG883
|
3.6
|
0.0
|
1.0
|
CB
|
J:CYS814
|
3.7
|
0.9
|
1.0
|
CB
|
J:CYS895
|
3.8
|
0.6
|
1.0
|
CA
|
J:CYS898
|
4.0
|
0.7
|
1.0
|
N
|
J:CYS898
|
4.0
|
0.0
|
1.0
|
C
|
J:CYS888
|
4.1
|
0.5
|
1.0
|
N
|
J:ASP889
|
4.2
|
0.2
|
1.0
|
N
|
J:CYS888
|
4.4
|
0.8
|
1.0
|
N
|
J:CYS814
|
4.4
|
0.2
|
1.0
|
N
|
J:CYS895
|
4.5
|
0.2
|
1.0
|
CG2
|
J:THR816
|
4.5
|
0.7
|
1.0
|
CZ
|
J:ARG883
|
4.6
|
0.8
|
1.0
|
O
|
J:CYS895
|
4.6
|
0.8
|
1.0
|
CA
|
J:CYS895
|
4.6
|
0.0
|
1.0
|
NE
|
J:ARG883
|
4.7
|
0.2
|
1.0
|
CA
|
J:CYS814
|
4.7
|
0.5
|
1.0
|
OG1
|
J:THR890
|
4.7
|
0.6
|
1.0
|
C
|
J:CYS895
|
4.9
|
0.3
|
1.0
|
|
Zinc binding site 5 out
of 6 in 5w1t
Go back to
Zinc Binding Sites List in 5w1t
Zinc binding site 5 out
of 6 in the X-Ray Crystal Structure of Escherichia Coli Rna Polymerase and Dksa Complex
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 5 of X-Ray Crystal Structure of Escherichia Coli Rna Polymerase and Dksa Complex within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
M:Zn200
b:0.0
occ:1.01
|
SG
|
M:CYS138
|
2.4
|
0.2
|
1.0
|
SG
|
M:CYS114
|
2.4
|
0.4
|
1.0
|
SG
|
M:CYS135
|
2.4
|
0.4
|
1.0
|
SG
|
M:CYS117
|
2.5
|
0.2
|
1.0
|
CB
|
M:CYS138
|
3.2
|
0.2
|
1.0
|
CB
|
M:CYS114
|
3.5
|
0.6
|
1.0
|
CB
|
M:CYS135
|
3.8
|
0.8
|
1.0
|
CB
|
M:CYS117
|
4.1
|
0.6
|
1.0
|
N
|
M:CYS135
|
4.1
|
0.0
|
1.0
|
N
|
M:CYS117
|
4.2
|
0.1
|
1.0
|
N
|
M:CYS138
|
4.3
|
0.8
|
1.0
|
CA
|
M:CYS138
|
4.4
|
0.2
|
1.0
|
CA
|
M:CYS135
|
4.5
|
1.0
|
1.0
|
O
|
M:CYS117
|
4.6
|
0.9
|
1.0
|
CA
|
M:CYS117
|
4.7
|
0.2
|
1.0
|
CB
|
M:SER116
|
4.7
|
0.8
|
1.0
|
CB
|
M:VAL119
|
4.9
|
0.7
|
1.0
|
CA
|
M:CYS114
|
5.0
|
0.4
|
1.0
|
O
|
M:CYS135
|
5.0
|
0.5
|
1.0
|
|
Zinc binding site 6 out
of 6 in 5w1t
Go back to
Zinc Binding Sites List in 5w1t
Zinc binding site 6 out
of 6 in the X-Ray Crystal Structure of Escherichia Coli Rna Polymerase and Dksa Complex
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 6 of X-Ray Crystal Structure of Escherichia Coli Rna Polymerase and Dksa Complex within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
N:Zn200
b:0.5
occ:1.01
|
SG
|
N:CYS135
|
2.3
|
0.0
|
1.0
|
SG
|
N:CYS138
|
2.4
|
0.9
|
1.0
|
SG
|
N:CYS114
|
2.4
|
0.5
|
1.0
|
SG
|
N:CYS117
|
2.5
|
0.3
|
1.0
|
CB
|
N:CYS138
|
2.8
|
0.5
|
1.0
|
CB
|
N:CYS135
|
3.5
|
0.6
|
1.0
|
CB
|
N:CYS114
|
3.7
|
0.5
|
1.0
|
N
|
N:CYS135
|
3.8
|
0.9
|
1.0
|
CB
|
N:CYS117
|
3.9
|
0.5
|
1.0
|
N
|
N:CYS117
|
4.0
|
0.6
|
1.0
|
N
|
N:CYS138
|
4.0
|
0.1
|
1.0
|
CA
|
N:CYS138
|
4.0
|
0.6
|
1.0
|
CA
|
N:CYS135
|
4.2
|
0.5
|
1.0
|
CB
|
N:SER116
|
4.3
|
0.6
|
1.0
|
CA
|
N:CYS117
|
4.5
|
0.7
|
1.0
|
O
|
N:CYS117
|
4.7
|
0.2
|
1.0
|
O
|
N:CYS135
|
4.7
|
0.5
|
1.0
|
C
|
N:CYS135
|
4.8
|
0.8
|
1.0
|
OG
|
N:SER116
|
4.8
|
0.8
|
1.0
|
CB
|
N:LEU134
|
4.9
|
0.6
|
1.0
|
C
|
N:LEU134
|
4.9
|
0.0
|
1.0
|
C
|
N:SER116
|
4.9
|
0.9
|
1.0
|
CA
|
N:SER116
|
5.0
|
0.3
|
1.0
|
CA
|
N:LEU134
|
5.0
|
0.9
|
1.0
|
N
|
N:SER116
|
5.0
|
0.3
|
1.0
|
|
Reference:
V.Molodtsov,
E.Sineva,
L.Zhang,
X.Huang,
M.Cashel,
S.E.Ades,
K.S.Murakami.
Allosteric Effector Ppgpp Potentiates the Inhibition of Transcript Initiation By Dksa. Mol. Cell V. 69 828 2018.
ISSN: ISSN 1097-4164
PubMed: 29478808
DOI: 10.1016/J.MOLCEL.2018.01.035
Page generated: Mon Oct 28 13:37:26 2024
|