Atomistry » Zinc » PDB 5w0m-5w97 » 5w1s
Atomistry »
  Zinc »
    PDB 5w0m-5w97 »
      5w1s »

Zinc in PDB 5w1s: X-Ray Crystal Structure of Escherichia Coli Rna Polymerase and Trar Complex

Enzymatic activity of X-Ray Crystal Structure of Escherichia Coli Rna Polymerase and Trar Complex

All present enzymatic activity of X-Ray Crystal Structure of Escherichia Coli Rna Polymerase and Trar Complex:
2.7.7.6;

Protein crystallography data

The structure of X-Ray Crystal Structure of Escherichia Coli Rna Polymerase and Trar Complex, PDB code: 5w1s was solved by K.S.Murakami, V.Molodtsov, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 49.84 / 3.81
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 186.484, 206.039, 310.300, 90.00, 90.00, 90.00
R / Rfree (%) 20.8 / 26.2

Other elements in 5w1s:

The structure of X-Ray Crystal Structure of Escherichia Coli Rna Polymerase and Trar Complex also contains other interesting chemical elements:

Magnesium (Mg) 2 atoms

Zinc Binding Sites:

The binding sites of Zinc atom in the X-Ray Crystal Structure of Escherichia Coli Rna Polymerase and Trar Complex (pdb code 5w1s). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 6 binding sites of Zinc where determined in the X-Ray Crystal Structure of Escherichia Coli Rna Polymerase and Trar Complex, PDB code: 5w1s:
Jump to Zinc binding site number: 1; 2; 3; 4; 5; 6;

Zinc binding site 1 out of 6 in 5w1s

Go back to Zinc Binding Sites List in 5w1s
Zinc binding site 1 out of 6 in the X-Ray Crystal Structure of Escherichia Coli Rna Polymerase and Trar Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of X-Ray Crystal Structure of Escherichia Coli Rna Polymerase and Trar Complex within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Zn2002

b:0.1
occ:1.00
SG D:CYS70 2.3 0.8 1.0
SG D:CYS72 2.4 0.3 1.0
SG D:CYS85 2.4 0.2 1.0
SG D:CYS88 2.6 0.2 1.0
CB D:CYS72 3.0 0.3 1.0
CB D:CYS85 3.1 0.1 1.0
N D:CYS72 3.4 0.0 1.0
CA D:CYS72 3.8 0.3 1.0
N D:CYS88 3.9 1.0 1.0
CB D:LYS87 3.9 0.1 1.0
CB D:CYS70 4.0 0.1 1.0
O D:CYS88 4.1 0.7 1.0
CB D:CYS88 4.2 1.0 1.0
N D:LEU71 4.3 0.7 1.0
N D:GLY73 4.4 0.4 1.0
CA D:CYS88 4.5 0.6 1.0
CA D:CYS85 4.5 1.0 1.0
C D:LEU71 4.5 0.7 1.0
C D:CYS72 4.5 0.2 1.0
CA D:LYS87 4.8 0.8 1.0
C D:CYS88 4.8 0.2 1.0
C D:CYS70 4.8 0.7 1.0
C D:LYS87 4.8 0.4 1.0
N D:LYS74 4.8 0.9 1.0
CA D:LEU71 4.8 0.4 1.0
N D:LYS87 4.9 0.6 1.0
CB D:LYS74 4.9 0.1 1.0
C D:CYS85 4.9 0.8 1.0
CG D:LYS87 4.9 0.0 1.0
CA D:CYS70 4.9 0.0 1.0

Zinc binding site 2 out of 6 in 5w1s

Go back to Zinc Binding Sites List in 5w1s
Zinc binding site 2 out of 6 in the X-Ray Crystal Structure of Escherichia Coli Rna Polymerase and Trar Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of X-Ray Crystal Structure of Escherichia Coli Rna Polymerase and Trar Complex within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Zn2003

b:0.4
occ:1.00
SG D:CYS895 2.2 0.5 1.0
SG D:CYS888 2.3 0.9 1.0
SG D:CYS898 2.4 1.0 1.0
SG D:CYS814 2.5 0.9 1.0
CB D:CYS888 2.9 0.9 1.0
CB D:CYS898 3.1 0.9 1.0
CB D:CYS895 3.4 0.7 1.0
CA D:CYS888 3.5 0.8 1.0
CB D:CYS814 3.6 0.1 1.0
NH2 D:ARG883 3.9 0.7 1.0
N D:CYS895 4.0 0.5 1.0
CA D:CYS895 4.2 0.0 1.0
C D:CYS888 4.2 0.1 1.0
N D:CYS898 4.2 0.8 1.0
N D:ASP889 4.2 0.1 1.0
CA D:CYS898 4.3 0.1 1.0
N D:CYS814 4.3 0.9 1.0
O D:CYS895 4.4 0.7 1.0
CA D:CYS814 4.6 0.5 1.0
C D:CYS895 4.7 0.8 1.0
N D:CYS888 4.7 0.2 1.0
N D:THR890 4.8 0.2 1.0
CG2 D:THR816 4.8 0.6 1.0
CB D:THR890 4.9 0.7 1.0
CZ D:ARG883 5.0 0.5 1.0

Zinc binding site 3 out of 6 in 5w1s

Go back to Zinc Binding Sites List in 5w1s
Zinc binding site 3 out of 6 in the X-Ray Crystal Structure of Escherichia Coli Rna Polymerase and Trar Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of X-Ray Crystal Structure of Escherichia Coli Rna Polymerase and Trar Complex within 5.0Å range:
probe atom residue distance (Å) B Occ
J:Zn2002

b:0.4
occ:1.00
SG J:CYS70 2.3 0.7 1.0
SG J:CYS72 2.3 0.3 1.0
SG J:CYS85 2.4 0.2 1.0
SG J:CYS88 2.5 0.4 1.0
CB J:CYS85 2.9 0.9 1.0
CB J:CYS72 3.2 0.2 1.0
N J:CYS72 3.6 0.8 1.0
CB J:CYS70 3.7 0.6 1.0
O J:CYS88 3.7 0.1 1.0
CA J:CYS72 3.9 0.0 1.0
N J:CYS88 3.9 0.0 1.0
CB J:CYS88 4.2 0.2 1.0
CB J:LYS87 4.3 0.7 1.0
N J:GLY73 4.3 0.4 1.0
CA J:CYS85 4.3 0.6 1.0
N J:LEU71 4.4 0.2 1.0
CA J:CYS88 4.5 0.9 1.0
C J:CYS88 4.5 0.4 1.0
C J:CYS72 4.5 0.9 1.0
C J:CYS70 4.7 0.3 1.0
C J:LEU71 4.7 0.2 1.0
N J:LYS74 4.7 0.2 1.0
CA J:CYS70 4.7 0.8 1.0
C J:CYS85 4.9 0.3 1.0
CB J:LYS74 4.9 0.2 1.0
CB J:VAL90 4.9 1.0 1.0
C J:LYS87 5.0 0.1 1.0

Zinc binding site 4 out of 6 in 5w1s

Go back to Zinc Binding Sites List in 5w1s
Zinc binding site 4 out of 6 in the X-Ray Crystal Structure of Escherichia Coli Rna Polymerase and Trar Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of X-Ray Crystal Structure of Escherichia Coli Rna Polymerase and Trar Complex within 5.0Å range:
probe atom residue distance (Å) B Occ
J:Zn2003

b:0.5
occ:1.00
SG J:CYS895 2.2 0.5 1.0
SG J:CYS888 2.4 0.8 1.0
SG J:CYS814 2.5 0.3 1.0
CB J:CYS898 2.6 0.5 1.0
CB J:CYS888 2.8 0.3 1.0
SG J:CYS898 2.8 0.3 1.0
CA J:CYS888 3.4 0.7 1.0
CB J:CYS895 3.6 0.2 1.0
CA J:CYS898 3.8 0.3 1.0
N J:CYS898 3.8 0.0 1.0
NH2 J:ARG883 3.9 0.4 1.0
CB J:CYS814 3.9 0.3 1.0
C J:CYS888 4.2 0.2 1.0
N J:ASP889 4.3 0.2 1.0
N J:CYS895 4.3 0.9 1.0
O J:CYS895 4.4 0.6 1.0
CA J:CYS895 4.4 0.8 1.0
CG2 J:THR816 4.4 0.2 1.0
OG1 J:THR890 4.4 0.8 1.0
N J:CYS814 4.4 0.6 1.0
N J:CYS888 4.5 0.7 1.0
C J:CYS895 4.7 0.1 1.0
CA J:CYS814 4.8 0.3 1.0
CZ J:ARG883 4.9 0.4 1.0
NE J:ARG883 4.9 0.7 1.0
C J:CYS898 5.0 0.2 1.0
OD1 J:ASP813 5.0 0.7 1.0

Zinc binding site 5 out of 6 in 5w1s

Go back to Zinc Binding Sites List in 5w1s
Zinc binding site 5 out of 6 in the X-Ray Crystal Structure of Escherichia Coli Rna Polymerase and Trar Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 5 of X-Ray Crystal Structure of Escherichia Coli Rna Polymerase and Trar Complex within 5.0Å range:
probe atom residue distance (Å) B Occ
M:Zn101

b:0.3
occ:1.00
SG M:CYS40 2.3 0.1 1.0
SG M:CYS58 2.3 0.9 1.0
SG M:CYS61 2.4 0.6 1.0
SG M:CYS37 2.4 0.2 1.0
CB M:CYS37 3.3 0.1 1.0
CB M:CYS58 3.6 1.0 1.0
CB M:CYS61 3.8 0.5 1.0
CB M:CYS40 3.9 0.2 1.0
CB M:ASN42 4.1 1.0 1.0
ND2 M:ASN42 4.1 0.5 1.0
N M:CYS40 4.2 0.6 1.0
N M:CYS58 4.2 0.5 1.0
N M:ASN42 4.3 0.4 1.0
CG M:ASN42 4.3 0.4 1.0
CA M:CYS40 4.4 0.8 1.0
CA M:CYS58 4.5 0.7 1.0
CB M:ALA39 4.6 0.5 1.0
N M:CYS61 4.6 0.6 1.0
C M:CYS40 4.6 0.2 1.0
N M:GLY41 4.7 0.2 1.0
CA M:ASN42 4.8 0.1 1.0
CA M:CYS37 4.8 0.5 1.0
CA M:CYS61 4.8 0.1 1.0

Zinc binding site 6 out of 6 in 5w1s

Go back to Zinc Binding Sites List in 5w1s
Zinc binding site 6 out of 6 in the X-Ray Crystal Structure of Escherichia Coli Rna Polymerase and Trar Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 6 of X-Ray Crystal Structure of Escherichia Coli Rna Polymerase and Trar Complex within 5.0Å range:
probe atom residue distance (Å) B Occ
N:Zn101

b:0.7
occ:1.00
SG N:CYS40 2.3 0.1 1.0
SG N:CYS61 2.3 0.8 1.0
SG N:CYS58 2.3 0.0 1.0
SG N:CYS37 2.3 0.2 1.0
CB N:CYS61 3.4 0.2 1.0
CB N:CYS37 3.6 0.7 1.0
CB N:CYS58 3.6 0.4 1.0
CB N:ALA39 3.7 0.3 1.0
N N:CYS58 4.0 0.8 1.0
CB N:CYS40 4.1 0.1 1.0
N N:CYS40 4.1 1.0 1.0
N N:CYS61 4.4 0.3 1.0
CA N:CYS58 4.4 0.4 1.0
CA N:CYS61 4.5 0.2 1.0
CA N:ALA39 4.6 0.0 1.0
N N:ALA39 4.6 0.3 1.0
CA N:CYS40 4.7 0.5 1.0
C N:ALA39 4.7 0.8 1.0
O N:CYS58 4.8 0.0 1.0
ND2 N:ASN42 4.9 0.3 1.0
CB N:ASN42 5.0 0.4 1.0
C N:CYS58 5.0 0.2 1.0

Reference:

V.Molodtsov, E.Sineva, L.Zhang, X.Huang, M.Cashel, S.E.Ades, K.S.Murakami. Allosteric Effector Ppgpp Potentiates the Inhibition of Transcript Initiation By Dksa. Mol. Cell V. 69 828 2018.
ISSN: ISSN 1097-4164
PubMed: 29478808
DOI: 10.1016/J.MOLCEL.2018.01.035
Page generated: Mon Oct 28 13:37:25 2024

Last articles

Zn in 9JYW
Zn in 9IR4
Zn in 9IR3
Zn in 9GMX
Zn in 9GMW
Zn in 9JEJ
Zn in 9ERF
Zn in 9ERE
Zn in 9EGV
Zn in 9EGW
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy