Zinc in PDB 5tl6: Crystal Structure of Sars-Cov Papain-Like Protease in Complex with the C-Terminal Domain of Human ISG15

All present enzymatic activity of Crystal Structure of Sars-Cov Papain-Like Protease in Complex with the C-Terminal Domain of Human ISG15:
2.7.7.48; 3.4.19.12; 3.4.22.69; 3.6.4.12; 3.6.4.13;

The structure of Crystal Structure of Sars-Cov Papain-Like Protease in Complex with the C-Terminal Domain of Human ISG15, PDB code: 5tl6 was solved by J.V.Dzimianski, C.M.Daczkowski, S.D.Pegan, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	45.87 / 2.62
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	46.881, 86.988, 221.532, 90.00, 90.00, 90.00
R / Rfree (%)	19.1 / 25

The binding sites of Zinc atom in the Crystal Structure of Sars-Cov Papain-Like Protease in Complex with the C-Terminal Domain of Human ISG15 (pdb code 5tl6). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of Sars-Cov Papain-Like Protease in Complex with the C-Terminal Domain of Human ISG15, PDB code: 5tl6:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of Sars-Cov Papain-Like Protease in Complex with the C-Terminal Domain of Human ISG15


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Sars-Cov Papain-Like Protease in Complex with the C-Terminal Domain of Human ISG15 within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn401 b:94.6 occ:1.00 SG B:CYS225 2.4 0.5 1.0 SG B:CYS190 2.6 76.0 1.0 SG B:CYS227 2.6 94.2 1.0 SG B:CYS193 2.9 81.1 1.0 CB B:CYS193 3.1 67.2 1.0 CB B:CYS227 3.4 93.3 1.0 CB B:CYS190 3.4 71.4 1.0 N B:CYS193 3.7 82.8 1.0 CB B:CYS225 3.7 92.8 1.0 CA B:CYS193 4.0 72.9 1.0 CB B:HIS192 4.4 75.9 1.0 N B:CYS227 4.5 96.9 1.0 CA B:CYS227 4.5 92.6 1.0 ND1 B:HIS192 4.6 91.2 1.0 CD B:ARG229 4.7 93.8 1.0 C B:HIS192 4.7 77.1 1.0 CB B:ARG229 4.8 82.2 1.0 CG B:HIS192 4.9 88.3 1.0 CA B:CYS190 4.9 72.3 1.0 CA B:HIS192 5.0 71.7 1.0 C B:CYS193 5.0 69.0 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of Sars-Cov Papain-Like Protease in Complex with the C-Terminal Domain of Human ISG15


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Sars-Cov Papain-Like Protease in Complex with the C-Terminal Domain of Human ISG15 within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn401 b:48.1 occ:1.00 SG D:CYS225 2.4 45.1 1.0 SG D:CYS227 2.4 45.8 1.0 SG D:CYS190 2.4 45.3 1.0 SG D:CYS193 2.5 45.0 1.0 CB D:CYS225 3.2 47.8 1.0 CB D:CYS227 3.3 41.1 1.0 CB D:CYS190 3.3 40.7 1.0 CB D:CYS193 3.4 47.9 1.0 N D:CYS193 4.0 51.5 1.0 N D:CYS227 4.2 44.2 1.0 CA D:CYS193 4.3 49.4 1.0 CA D:CYS227 4.3 43.1 1.0 CA D:CYS225 4.6 37.9 1.0 CB D:HIS192 4.7 53.3 1.0 ND1 D:HIS192 4.7 54.7 1.0 CA D:CYS190 4.8 41.0 1.0 NH1 D:ARG229 4.8 48.7 1.0 CB D:ARG229 4.9 36.5 1.0 C D:CYS225 4.9 40.9 1.0 C D:HIS192 4.9 53.1 1.0 CD D:ARG229 4.9 48.9 1.0

C.M.Daczkowski, J.V.Dzimianski, J.R.Clasman, O.Goodwin, A.D.Mesecar, S.D.Pegan. Structural Insights Into the Interaction of Coronavirus Papain-Like Proteases and Interferon-Stimulated Gene Product 15 From Different Species. J. Mol. Biol. V. 429 1661 2017.
ISSN: ESSN 1089-8638
PubMed: 28438633
DOI: 10.1016/J.JMB.2017.04.011