Zinc in PDB 5m3f: Yeast Rna Polymerase I Elongation Complex at 3.8A
Enzymatic activity of Yeast Rna Polymerase I Elongation Complex at 3.8A
All present enzymatic activity of Yeast Rna Polymerase I Elongation Complex at 3.8A:
2.7.7.6;
Other elements in 5m3f:
The structure of Yeast Rna Polymerase I Elongation Complex at 3.8A also contains other interesting chemical elements:
Zinc Binding Sites:
The binding sites of Zinc atom in the Yeast Rna Polymerase I Elongation Complex at 3.8A
(pdb code 5m3f). This binding sites where shown within
5.0 Angstroms radius around Zinc atom.
In total 6 binding sites of Zinc where determined in the
Yeast Rna Polymerase I Elongation Complex at 3.8A, PDB code: 5m3f:
Jump to Zinc binding site number:
1;
2;
3;
4;
5;
6;
Zinc binding site 1 out
of 6 in 5m3f
Go back to
Zinc Binding Sites List in 5m3f
Zinc binding site 1 out
of 6 in the Yeast Rna Polymerase I Elongation Complex at 3.8A
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 1 of Yeast Rna Polymerase I Elongation Complex at 3.8A within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Zn3001
b:75.2
occ:1.00
|
CD2
|
A:HIS75
|
2.1
|
57.5
|
1.0
|
NE2
|
A:HIS75
|
2.2
|
58.8
|
1.0
|
CB
|
A:CYS65
|
2.2
|
61.9
|
1.0
|
SG
|
A:CYS65
|
2.3
|
55.2
|
1.0
|
SG
|
A:CYS72
|
2.3
|
61.7
|
1.0
|
SG
|
A:CYS62
|
2.3
|
59.2
|
1.0
|
N
|
A:CYS65
|
2.7
|
71.8
|
1.0
|
CA
|
A:CYS65
|
3.0
|
70.8
|
1.0
|
CE1
|
A:HIS75
|
3.5
|
61.6
|
1.0
|
CG
|
A:HIS75
|
3.5
|
56.6
|
1.0
|
C
|
A:THR64
|
3.9
|
67.2
|
1.0
|
CB
|
A:THR64
|
3.9
|
58.7
|
1.0
|
CB
|
A:CYS62
|
4.0
|
64.9
|
1.0
|
ND1
|
A:HIS75
|
4.1
|
58.6
|
1.0
|
CB
|
A:CYS72
|
4.1
|
53.8
|
1.0
|
C
|
A:CYS65
|
4.2
|
81.1
|
1.0
|
CG2
|
A:THR64
|
4.3
|
58.9
|
1.0
|
CA
|
A:GLY55
|
4.3
|
68.8
|
1.0
|
CA
|
A:THR64
|
4.3
|
59.9
|
1.0
|
N
|
A:GLY66
|
4.5
|
59.5
|
1.0
|
O
|
A:LEU54
|
4.6
|
63.1
|
1.0
|
CB
|
A:HIS75
|
4.6
|
51.1
|
1.0
|
N
|
A:THR64
|
4.6
|
64.3
|
1.0
|
N
|
A:GLY74
|
4.8
|
56.6
|
1.0
|
O
|
A:THR64
|
4.9
|
66.7
|
1.0
|
CA
|
A:GLY74
|
4.9
|
60.3
|
1.0
|
N
|
A:HIS75
|
4.9
|
55.3
|
1.0
|
C
|
A:GLY74
|
5.0
|
60.5
|
1.0
|
CA
|
A:CYS72
|
5.0
|
53.1
|
1.0
|
|
Zinc binding site 2 out
of 6 in 5m3f
Go back to
Zinc Binding Sites List in 5m3f
Zinc binding site 2 out
of 6 in the Yeast Rna Polymerase I Elongation Complex at 3.8A
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 2 of Yeast Rna Polymerase I Elongation Complex at 3.8A within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Zn3002
b:77.5
occ:1.00
|
SG
|
A:CYS105
|
2.3
|
72.6
|
1.0
|
SG
|
A:CYS236
|
2.3
|
79.3
|
1.0
|
SG
|
A:CYS233
|
2.3
|
73.0
|
1.0
|
SG
|
A:CYS102
|
2.3
|
75.2
|
1.0
|
CB
|
A:CYS105
|
2.8
|
74.3
|
1.0
|
N
|
A:CYS236
|
3.3
|
86.4
|
1.0
|
CB
|
A:CYS236
|
3.4
|
80.5
|
1.0
|
N
|
A:CYS105
|
3.5
|
81.3
|
1.0
|
CB
|
A:CYS233
|
3.7
|
81.4
|
1.0
|
CB
|
A:ASN235
|
3.7
|
79.7
|
1.0
|
CA
|
A:CYS105
|
3.8
|
73.5
|
1.0
|
CB
|
A:CYS102
|
3.9
|
77.8
|
1.0
|
CA
|
A:CYS236
|
4.0
|
86.5
|
1.0
|
C
|
A:ASN235
|
4.4
|
84.7
|
1.0
|
CA
|
A:ASN235
|
4.5
|
82.5
|
1.0
|
N
|
A:ASN235
|
4.6
|
75.5
|
1.0
|
N
|
A:GLY237
|
4.6
|
91.9
|
1.0
|
ND2
|
A:ASN235
|
4.6
|
73.7
|
1.0
|
CG
|
A:ASN235
|
4.7
|
77.4
|
1.0
|
C
|
A:PHE104
|
4.7
|
80.0
|
1.0
|
CB
|
A:PHE104
|
4.7
|
76.7
|
1.0
|
C
|
A:CYS236
|
4.9
|
92.1
|
1.0
|
C
|
A:CYS105
|
4.9
|
75.7
|
1.0
|
CA
|
A:CYS233
|
5.0
|
80.0
|
1.0
|
|
Zinc binding site 3 out
of 6 in 5m3f
Go back to
Zinc Binding Sites List in 5m3f
Zinc binding site 3 out
of 6 in the Yeast Rna Polymerase I Elongation Complex at 3.8A
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 3 of Yeast Rna Polymerase I Elongation Complex at 3.8A within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Zn1302
b:53.6
occ:1.00
|
SG
|
B:CYS1131
|
2.3
|
75.4
|
1.0
|
SG
|
B:CYS1128
|
2.3
|
43.2
|
1.0
|
SG
|
B:CYS1107
|
2.3
|
53.4
|
1.0
|
SG
|
B:CYS1104
|
2.3
|
49.9
|
1.0
|
CB
|
B:CYS1131
|
2.7
|
66.3
|
1.0
|
CB
|
B:CYS1128
|
2.9
|
53.8
|
1.0
|
CB
|
B:CYS1107
|
3.3
|
31.5
|
1.0
|
N
|
B:CYS1131
|
3.5
|
53.4
|
1.0
|
CA
|
B:CYS1131
|
3.7
|
62.9
|
1.0
|
CB
|
B:CYS1104
|
3.8
|
50.2
|
1.0
|
N
|
B:CYS1107
|
4.1
|
44.8
|
1.0
|
CA
|
B:CYS1107
|
4.3
|
37.3
|
1.0
|
CA
|
B:CYS1128
|
4.3
|
48.6
|
1.0
|
C
|
B:ARG1130
|
4.6
|
62.2
|
1.0
|
CB
|
B:ARG1130
|
4.6
|
68.5
|
1.0
|
C
|
B:CYS1128
|
4.7
|
55.4
|
1.0
|
N
|
B:ARG1130
|
4.8
|
57.1
|
1.0
|
C
|
B:CYS1131
|
4.8
|
66.8
|
1.0
|
O
|
B:CYS1128
|
4.8
|
66.7
|
1.0
|
CA
|
B:ARG1130
|
4.9
|
64.0
|
1.0
|
CA
|
B:CYS1104
|
5.0
|
48.7
|
1.0
|
|
Zinc binding site 4 out
of 6 in 5m3f
Go back to
Zinc Binding Sites List in 5m3f
Zinc binding site 4 out
of 6 in the Yeast Rna Polymerase I Elongation Complex at 3.8A
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 4 of Yeast Rna Polymerase I Elongation Complex at 3.8A within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
I:Zn3001
b:74.0
occ:1.00
|
CB
|
I:CYS30
|
1.9
|
73.1
|
1.0
|
SG
|
I:CYS13
|
2.3
|
73.5
|
1.0
|
SG
|
I:CYS33
|
2.3
|
71.0
|
1.0
|
SG
|
I:CYS30
|
2.3
|
67.0
|
1.0
|
SG
|
I:CYS10
|
2.3
|
78.7
|
1.0
|
CB
|
I:CYS10
|
2.5
|
74.2
|
1.0
|
CA
|
I:CYS30
|
3.3
|
80.6
|
1.0
|
N
|
I:CYS30
|
3.9
|
84.5
|
1.0
|
CA
|
I:CYS10
|
4.1
|
76.6
|
1.0
|
CB
|
I:CYS13
|
4.1
|
85.7
|
1.0
|
CB
|
I:CYS33
|
4.1
|
74.0
|
1.0
|
N
|
I:CYS33
|
4.4
|
85.7
|
1.0
|
C
|
I:CYS30
|
4.4
|
78.0
|
1.0
|
N
|
I:CYS13
|
4.4
|
91.4
|
1.0
|
CB
|
I:ALA35
|
4.6
|
72.7
|
1.0
|
N
|
I:ALA35
|
4.6
|
86.7
|
1.0
|
CA
|
I:CYS13
|
4.7
|
85.0
|
1.0
|
CA
|
I:CYS33
|
4.7
|
82.2
|
1.0
|
O
|
I:CYS13
|
4.7
|
0.2
|
1.0
|
N
|
I:CYS10
|
4.8
|
73.3
|
1.0
|
N
|
I:LYS34
|
4.8
|
84.1
|
1.0
|
CD2
|
I:LEU17
|
4.8
|
71.7
|
1.0
|
C
|
I:CYS13
|
4.8
|
95.5
|
1.0
|
C
|
I:CYS10
|
4.9
|
79.3
|
1.0
|
N
|
I:SER31
|
5.0
|
71.7
|
1.0
|
|
Zinc binding site 5 out
of 6 in 5m3f
Go back to
Zinc Binding Sites List in 5m3f
Zinc binding site 5 out
of 6 in the Yeast Rna Polymerase I Elongation Complex at 3.8A
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 5 of Yeast Rna Polymerase I Elongation Complex at 3.8A within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
J:Zn3001
b:0.2
occ:1.01
|
CB
|
J:CYS10
|
2.2
|
56.0
|
1.0
|
SG
|
J:CYS10
|
2.3
|
55.2
|
1.0
|
SG
|
J:CYS46
|
2.3
|
61.8
|
1.0
|
SG
|
J:CYS45
|
2.3
|
99.9
|
1.0
|
SG
|
J:CYS7
|
2.3
|
59.1
|
1.0
|
CB
|
J:CYS7
|
3.0
|
60.2
|
1.0
|
N
|
J:CYS10
|
3.0
|
70.0
|
1.0
|
CA
|
J:CYS10
|
3.1
|
63.8
|
1.0
|
N
|
J:CYS46
|
3.5
|
84.7
|
1.0
|
CB
|
J:CYS46
|
3.5
|
75.7
|
1.0
|
CA
|
J:CYS46
|
3.5
|
79.5
|
1.0
|
CB
|
J:CYS45
|
3.7
|
53.5
|
1.0
|
C
|
J:CYS45
|
3.9
|
81.9
|
1.0
|
N
|
J:GLY11
|
4.0
|
72.9
|
1.0
|
C
|
J:CYS10
|
4.1
|
69.6
|
1.0
|
CA
|
J:CYS7
|
4.2
|
59.1
|
1.0
|
C
|
J:SER9
|
4.2
|
68.0
|
1.0
|
C
|
J:CYS7
|
4.3
|
59.0
|
1.0
|
O
|
J:CYS7
|
4.4
|
59.4
|
1.0
|
O
|
J:CYS45
|
4.4
|
75.8
|
1.0
|
CA
|
J:CYS45
|
4.4
|
81.3
|
1.0
|
N
|
J:SER9
|
4.6
|
63.5
|
1.0
|
CA
|
J:SER9
|
4.8
|
67.8
|
1.0
|
N
|
J:PHE8
|
4.8
|
53.1
|
1.0
|
CB
|
J:SER9
|
4.8
|
73.9
|
1.0
|
N
|
J:LYS12
|
4.9
|
68.5
|
1.0
|
|
Zinc binding site 6 out
of 6 in 5m3f
Go back to
Zinc Binding Sites List in 5m3f
Zinc binding site 6 out
of 6 in the Yeast Rna Polymerase I Elongation Complex at 3.8A
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 6 of Yeast Rna Polymerase I Elongation Complex at 3.8A within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
L:Zn3001
b:87.2
occ:1.00
|
SG
|
L:CYS34
|
2.3
|
85.1
|
1.0
|
CB
|
L:CYS51
|
2.4
|
87.7
|
1.0
|
SG
|
L:CYS51
|
2.5
|
0.1
|
1.0
|
N
|
L:CYS51
|
2.8
|
90.7
|
1.0
|
CA
|
L:CYS51
|
3.1
|
88.9
|
1.0
|
CB
|
L:CYS34
|
3.2
|
73.9
|
1.0
|
SG
|
L:CYS48
|
3.3
|
79.1
|
1.0
|
SG
|
L:CYS31
|
3.7
|
82.4
|
1.0
|
N
|
L:CYS34
|
3.9
|
82.0
|
1.0
|
C
|
L:ASP50
|
4.0
|
0.1
|
1.0
|
CB
|
L:CYS48
|
4.0
|
0.7
|
1.0
|
CB
|
L:ASP50
|
4.0
|
0.3
|
1.0
|
C
|
L:CYS51
|
4.1
|
94.1
|
1.0
|
CA
|
L:CYS34
|
4.2
|
79.3
|
1.0
|
N
|
L:GLY52
|
4.3
|
99.2
|
1.0
|
CA
|
L:ASP50
|
4.4
|
0.6
|
1.0
|
CB
|
L:HIS53
|
4.5
|
0.6
|
1.0
|
N
|
L:HIS53
|
4.5
|
99.0
|
1.0
|
N
|
L:ASP50
|
4.6
|
0.2
|
1.0
|
CB
|
L:CYS31
|
4.7
|
74.2
|
1.0
|
CB
|
L:GLU33
|
4.8
|
84.5
|
1.0
|
O
|
L:CYS51
|
5.0
|
91.5
|
1.0
|
O
|
L:ASP50
|
5.0
|
0.8
|
1.0
|
|
Reference:
S.Neyer,
M.Kunz,
C.Geiss,
M.Hantsche,
V.V.Hodirnau,
A.Seybert,
C.Engel,
M.P.Scheffer,
P.Cramer,
A.S.Frangakis.
Structure of Rna Polymerase I Transcribing Ribosomal Dna Genes. Nature V. 540 607 2016.
ISSN: ISSN 0028-0836
PubMed: 27842382
DOI: 10.1038/NATURE20561
Page generated: Sun Oct 27 21:42:14 2024
|