Zinc in PDB 5lmx: Monomeric Rna Polymerase I at 4.9 A Resolution

All present enzymatic activity of Monomeric Rna Polymerase I at 4.9 A Resolution:
2.7.7.6;

The binding sites of Zinc atom in the Monomeric Rna Polymerase I at 4.9 A Resolution (pdb code 5lmx). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 6 binding sites of Zinc where determined in the Monomeric Rna Polymerase I at 4.9 A Resolution, PDB code: 5lmx:
Jump to Zinc binding site number: 1; 2; 3; 4; 5; 6;

Zinc binding site 1 out of 6 in the Monomeric Rna Polymerase I at 4.9 A Resolution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Monomeric Rna Polymerase I at 4.9 A Resolution within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1701 b:0.1 occ:1.00 SG A:CYS105 2.2 0.3 1.0 SG A:CYS233 2.4 0.2 1.0 SG A:CYS102 2.4 0.9 1.0 SG A:CYS236 2.4 0.8 1.0 CB A:CYS102 3.1 0.1 1.0 CB A:CYS233 3.3 0.3 1.0 CB A:CYS105 3.3 0.3 1.0 O A:CYS236 3.6 0.3 1.0 N A:CYS236 3.7 0.9 1.0 CB A:CYS236 3.7 0.9 1.0 N A:CYS105 3.8 1.0 1.0 CA A:CYS236 4.1 0.4 1.0 CA A:CYS105 4.1 0.9 1.0 C A:CYS236 4.3 0.8 1.0 CB A:ASN235 4.5 0.9 1.0 CA A:CYS102 4.6 0.4 1.0 CA A:CYS233 4.8 0.2 1.0 C A:ASN235 4.8 0.3 1.0 C A:CYS105 4.9 0.3 1.0 N A:HIS106 4.9 0.6 1.0 CB A:PHE104 5.0 0.1 1.0

Zinc binding site 2 out of 6 in the Monomeric Rna Polymerase I at 4.9 A Resolution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Monomeric Rna Polymerase I at 4.9 A Resolution within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1702 b:95.4 occ:1.00 NE2 A:HIS75 2.0 92.3 1.0 SG A:CYS65 2.2 92.3 1.0 SG A:CYS62 2.3 94.9 1.0 SG A:CYS72 2.3 92.9 1.0 CD2 A:HIS75 2.8 92.3 1.0 CB A:CYS62 3.0 90.8 1.0 CE1 A:HIS75 3.1 92.0 1.0 CB A:CYS65 3.3 89.3 1.0 N A:CYS65 3.7 90.1 1.0 CB A:CYS72 3.7 89.0 1.0 CG A:HIS75 4.0 90.5 1.0 CA A:CYS65 4.0 89.9 1.0 ND1 A:HIS75 4.1 92.2 1.0 CA A:GLY55 4.3 81.8 1.0 CD A:PRO73 4.4 90.2 1.0 CA A:CYS72 4.4 88.5 1.0 CA A:CYS62 4.5 91.1 1.0 N A:GLY66 4.6 89.8 1.0 C A:CYS65 4.6 93.2 1.0 N A:GLY74 4.7 85.8 1.0 CB A:THR64 4.7 93.7 1.0 C A:THR64 4.8 94.5 1.0 N A:PRO73 4.9 87.8 1.0 N A:ALA56 4.9 83.7 1.0 C A:CYS72 4.9 91.5 1.0 CA A:GLY74 5.0 85.5 1.0 N A:LEU67 5.0 89.2 1.0

Zinc binding site 3 out of 6 in the Monomeric Rna Polymerase I at 4.9 A Resolution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Monomeric Rna Polymerase I at 4.9 A Resolution within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn1301 b:79.3 occ:1.00 SG B:CYS1131 2.2 88.5 1.0 SG B:CYS1104 2.2 81.6 1.0 SG B:CYS1128 2.3 84.6 1.0 SG B:CYS1107 2.4 88.7 1.0 CB B:CYS1104 3.2 76.8 1.0 CB B:CYS1107 3.3 85.5 1.0 CB B:CYS1131 3.3 86.2 1.0 CB B:CYS1128 3.4 81.5 1.0 N B:CYS1107 3.7 85.1 1.0 N B:CYS1131 3.8 86.3 1.0 CA B:CYS1107 4.1 84.9 1.0 CA B:CYS1131 4.2 87.1 1.0 CB B:ARG1130 4.3 83.0 1.0 OG1 B:THR1112 4.3 0.9 1.0 CB B:GLU1106 4.4 86.4 1.0 CG B:ARG1130 4.6 89.1 1.0 C B:GLU1106 4.6 88.1 1.0 CA B:CYS1104 4.6 76.2 1.0 C B:ARG1130 4.8 89.9 1.0 OG B:SER1109 4.8 0.5 1.0 CA B:CYS1128 4.8 82.0 1.0 CA B:GLU1106 4.9 83.9 1.0 CA B:ARG1130 4.9 84.5 1.0 C B:CYS1107 4.9 89.3 1.0

Zinc binding site 4 out of 6 in the Monomeric Rna Polymerase I at 4.9 A Resolution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Monomeric Rna Polymerase I at 4.9 A Resolution within 5.0Å range: probe atom residue distance (Å) B Occ I:Zn201 b:0.0 occ:1.00 SG I:CYS30 2.2 0.7 1.0 SG I:CYS13 2.5 0.4 1.0 CB I:CYS30 2.8 0.9 1.0 SG I:CYS10 3.0 0.9 1.0 SG I:CYS33 3.1 0.3 1.0 CB I:CYS10 3.5 0.8 1.0 O I:CYS33 3.8 0.5 1.0 CB I:CYS13 4.1 0.7 1.0 N I:CYS33 4.2 0.5 1.0 CA I:CYS30 4.3 0.2 1.0 CB I:ALA35 4.5 0.3 1.0 CB I:CYS33 4.6 0.7 1.0 CB I:GLN32 4.6 0.2 1.0 CB I:ASP15 4.6 0.5 1.0 N I:CYS13 4.6 0.5 1.0 C I:CYS33 4.7 0.2 1.0 CA I:CYS33 4.7 0.4 1.0 C I:CYS30 4.9 0.7 1.0 CA I:CYS13 5.0 0.3 1.0 CA I:CYS10 5.0 0.1 1.0

Zinc binding site 5 out of 6 in the Monomeric Rna Polymerase I at 4.9 A Resolution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 5 of Monomeric Rna Polymerase I at 4.9 A Resolution within 5.0Å range: probe atom residue distance (Å) B Occ J:Zn101 b:0.1 occ:1.00 SG J:CYS7 2.2 1.0 1.0 SG J:CYS46 2.3 0.6 1.0 SG J:CYS45 2.3 0.2 1.0 SG J:CYS10 2.6 97.7 1.0 CB J:CYS7 3.1 99.2 1.0 N J:CYS46 3.3 97.3 1.0 CB J:CYS45 3.5 95.9 1.0 CB J:CYS46 3.6 98.9 1.0 CA J:CYS46 3.6 97.6 1.0 CB J:CYS10 3.7 92.5 1.0 C J:CYS45 3.7 0.5 1.0 N J:CYS10 3.7 90.8 1.0 CA J:CYS45 4.2 96.3 1.0 NE J:ARG43 4.2 84.9 1.0 O J:CYS45 4.2 99.9 1.0 CA J:CYS10 4.3 91.2 1.0 CB J:SER9 4.4 94.1 1.0 CA J:CYS7 4.5 98.1 1.0 N J:GLY11 4.7 93.0 1.0 C J:SER9 4.8 93.8 1.0 CB J:ARG43 4.8 95.6 1.0 C J:CYS7 4.8 98.7 1.0 O J:CYS7 4.9 98.0 1.0 CD J:ARG43 4.9 87.4 1.0 CB J:LYS12 4.9 1.0 1.0 NH2 J:ARG43 4.9 78.6 1.0 N J:SER9 4.9 91.3 1.0 N J:LYS12 5.0 97.4 1.0 CA J:SER9 5.0 90.3 1.0 N J:CYS45 5.0 97.5 1.0 C J:CYS10 5.0 95.7 1.0

Zinc binding site 6 out of 6 in the Monomeric Rna Polymerase I at 4.9 A Resolution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 6 of Monomeric Rna Polymerase I at 4.9 A Resolution within 5.0Å range: probe atom residue distance (Å) B Occ L:Zn101 b:0.5 occ:1.00 SG L:CYS48 1.5 0.2 1.0 SG L:CYS34 2.2 0.1 1.0 OG L:SER36 2.4 0.6 1.0 CB L:CYS48 3.1 0.5 1.0 CB L:ASP50 3.4 0.4 1.0 CB L:CYS31 3.5 0.4 1.0 SG L:CYS31 3.6 0.0 1.0 CB L:SER36 3.6 0.8 1.0 N L:CYS51 3.7 0.4 1.0 SG L:CYS51 3.9 0.6 1.0 N L:ASP50 4.0 0.9 1.0 CB L:CYS34 4.0 0.9 1.0 OD1 L:ASP50 4.1 0.9 1.0 CA L:ASP50 4.1 0.5 1.0 CG L:ASP50 4.1 0.8 1.0 CA L:CYS48 4.3 0.2 1.0 C L:ASP50 4.3 0.6 1.0 O L:CYS51 4.5 0.9 1.0 C L:CYS48 4.5 0.1 1.0 N L:SER36 4.5 0.2 1.0 N L:LYS49 4.5 0.1 1.0 CB L:CYS51 4.6 0.6 1.0 CA L:SER36 4.6 0.6 1.0 CA L:CYS51 4.6 0.5 1.0 O L:SER36 4.7 0.4 1.0 CA L:CYS34 4.9 0.7 1.0

E.Torreira, J.A.Louro, I.Pazos, N.Gonzalez-Polo, D.Gil-Carton, A.G.Duran, S.Tosi, O.Gallego, O.Calvo, C.Fernandez-Tornero. The Dynamic Assembly of Distinct Rna Polymerase I Complexes Modulates Rdna Transcription. Elife V. 6 2017.
ISSN: ESSN 2050-084X
PubMed: 28262097
DOI: 10.7554/ELIFE.20832