Zinc in PDB 5l1z: Tar Complex with Hiv-1 Tat-AFF4-P-Tefb

All present enzymatic activity of Tar Complex with Hiv-1 Tat-AFF4-P-Tefb:
2.7.11.22; 2.7.11.23;

The structure of Tar Complex with Hiv-1 Tat-AFF4-P-Tefb, PDB code: 5l1z was solved by U.Schulze-Gahmen, J.Hurley, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	500.00 / 5.90
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	146.870, 146.870, 103.750, 90.00, 90.00, 120.00
R / Rfree (%)	21.7 / 31.3

The binding sites of Zinc atom in the Tar Complex with Hiv-1 Tat-AFF4-P-Tefb (pdb code 5l1z). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Tar Complex with Hiv-1 Tat-AFF4-P-Tefb, PDB code: 5l1z:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Tar Complex with Hiv-1 Tat-AFF4-P-Tefb


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Tar Complex with Hiv-1 Tat-AFF4-P-Tefb within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn101 b:0.1 occ:1.01 SG D:CYS37 2.4 0.1 1.0 SG D:CYS34 2.4 0.9 1.0 SG D:CYS22 2.6 0.2 1.0 ND1 D:HIS33 2.8 0.4 1.0 CB D:CYS22 3.1 0.2 1.0 CG D:HIS33 3.3 0.4 1.0 CE1 D:HIS33 3.4 0.4 1.0 CB D:CYS34 3.4 0.9 1.0 CA D:CYS22 3.6 1.0 1.0 CB D:HIS33 3.7 0.4 1.0 CB D:CYS37 3.8 0.1 1.0 N D:CYS34 4.0 0.7 1.0 NE2 D:HIS33 4.2 0.4 1.0 CD2 D:HIS33 4.2 0.4 1.0 CA D:CYS34 4.3 0.7 1.0 C D:HIS33 4.3 0.0 1.0 N D:CYS37 4.5 0.2 1.0 N D:CYS22 4.6 1.0 1.0 C D:CYS22 4.6 1.0 1.0 CA D:HIS33 4.7 0.0 1.0 CA D:CYS37 4.7 0.2 1.0 CG D:PRO18 4.8 0.6 1.0 O D:HIS33 4.9 0.0 1.0 N D:THR23 5.0 0.9 1.0

Zinc binding site 2 out of 2 in the Tar Complex with Hiv-1 Tat-AFF4-P-Tefb


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Tar Complex with Hiv-1 Tat-AFF4-P-Tefb within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn102 b:0.5 occ:1.01 SG D:CYS25 2.4 0.9 1.0 CB D:CYS25 2.5 0.9 1.0 SG D:CYS27 2.6 0.3 1.0 SG B:CYS261 2.6 0.7 1.0 SG D:CYS30 2.7 0.6 1.0 CB B:CYS261 3.3 0.7 1.0 CB D:CYS27 3.7 0.3 1.0 CA B:CYS261 3.9 0.1 1.0 CA D:CYS25 4.0 1.0 1.0 CB D:CYS30 4.1 0.6 1.0 O B:ALA260 4.3 0.1 1.0 C D:CYS25 4.4 1.0 1.0 N D:CYS27 4.5 0.7 1.0 N D:CYS25 4.7 1.0 1.0 O D:CYS25 4.7 1.0 1.0 CA D:CYS27 4.7 0.7 1.0 N B:CYS261 4.8 0.1 1.0 C B:ALA260 4.9 0.1 1.0 N D:CYS30 4.9 0.5 1.0 OG1 D:THR23 4.9 0.9 1.0

U.Schulze-Gahmen, I.Echeverria, G.Stjepanovic, Y.Bai, H.Lu, D.Schneidman-Duhovny, J.A.Doudna, Q.Zhou, A.Sali, J.H.Hurley. Insights Into Hiv-1 Proviral Transcription From Integrative Structure and Dynamics of the Tat:AFF4:P-Tefb:Tar Complex. Elife V. 5 2016.
ISSN: ESSN 2050-084X
PubMed: 27731797
DOI: 10.7554/ELIFE.15910