Zinc in PDB 5i2d: Crystal Structure of T. Thermophilus TTHB099 Class II Transcription Activation Complex: Tap-Rpo
Enzymatic activity of Crystal Structure of T. Thermophilus TTHB099 Class II Transcription Activation Complex: Tap-Rpo
All present enzymatic activity of Crystal Structure of T. Thermophilus TTHB099 Class II Transcription Activation Complex: Tap-Rpo:
2.7.7.6;
Protein crystallography data
The structure of Crystal Structure of T. Thermophilus TTHB099 Class II Transcription Activation Complex: Tap-Rpo, PDB code: 5i2d
was solved by
Y.Feng,
Y.Zhang,
R.H.Ebright,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
49.31 /
4.41
|
Space group
|
P 1 21 1
|
Cell size a, b, c (Å), α, β, γ (°)
|
171.487,
105.446,
374.585,
90.00,
102.39,
90.00
|
R / Rfree (%)
|
24.1 /
28.4
|
Other elements in 5i2d:
The structure of Crystal Structure of T. Thermophilus TTHB099 Class II Transcription Activation Complex: Tap-Rpo also contains other interesting chemical elements:
Zinc Binding Sites:
The binding sites of Zinc atom in the Crystal Structure of T. Thermophilus TTHB099 Class II Transcription Activation Complex: Tap-Rpo
(pdb code 5i2d). This binding sites where shown within
5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the
Crystal Structure of T. Thermophilus TTHB099 Class II Transcription Activation Complex: Tap-Rpo, PDB code: 5i2d:
Jump to Zinc binding site number:
1;
2;
3;
4;
Zinc binding site 1 out
of 4 in 5i2d
Go back to
Zinc Binding Sites List in 5i2d
Zinc binding site 1 out
of 4 in the Crystal Structure of T. Thermophilus TTHB099 Class II Transcription Activation Complex: Tap-Rpo
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 1 of Crystal Structure of T. Thermophilus TTHB099 Class II Transcription Activation Complex: Tap-Rpo within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:Zn2001
b:52.6
occ:1.00
|
SG
|
D:CYS1112
|
2.3
|
63.2
|
1.0
|
SG
|
D:CYS1201
|
2.3
|
77.4
|
1.0
|
SG
|
D:CYS1194
|
2.3
|
74.2
|
1.0
|
SG
|
D:CYS1204
|
2.3
|
0.8
|
1.0
|
CB
|
D:CYS1204
|
3.2
|
0.6
|
1.0
|
CB
|
D:CYS1194
|
3.2
|
74.8
|
1.0
|
CA
|
D:CYS1194
|
3.5
|
73.9
|
1.0
|
CB
|
D:CYS1112
|
3.6
|
62.8
|
1.0
|
CB
|
D:CYS1201
|
3.6
|
89.3
|
1.0
|
OG1
|
D:THR1196
|
3.8
|
0.2
|
1.0
|
N
|
D:GLN1195
|
3.9
|
60.8
|
1.0
|
C
|
D:CYS1194
|
4.2
|
76.3
|
1.0
|
N
|
D:CYS1112
|
4.2
|
68.6
|
1.0
|
N
|
D:CYS1201
|
4.2
|
87.1
|
1.0
|
CG2
|
D:THR1114
|
4.2
|
78.4
|
1.0
|
CA
|
D:CYS1204
|
4.4
|
0.4
|
1.0
|
N
|
D:CYS1204
|
4.5
|
0.2
|
1.0
|
CA
|
D:CYS1201
|
4.5
|
85.4
|
1.0
|
CA
|
D:CYS1112
|
4.5
|
69.1
|
1.0
|
N
|
D:THR1196
|
4.6
|
98.5
|
1.0
|
NH2
|
D:ARG1189
|
4.7
|
0.9
|
1.0
|
N
|
D:CYS1194
|
4.7
|
69.7
|
1.0
|
CB
|
D:THR1196
|
4.9
|
0.8
|
1.0
|
|
Zinc binding site 2 out
of 4 in 5i2d
Go back to
Zinc Binding Sites List in 5i2d
Zinc binding site 2 out
of 4 in the Crystal Structure of T. Thermophilus TTHB099 Class II Transcription Activation Complex: Tap-Rpo
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 2 of Crystal Structure of T. Thermophilus TTHB099 Class II Transcription Activation Complex: Tap-Rpo within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:Zn2002
b:49.2
occ:1.00
|
SG
|
D:CYS73
|
2.3
|
62.6
|
1.0
|
SG
|
D:CYS76
|
2.3
|
86.4
|
1.0
|
SG
|
D:CYS60
|
2.3
|
51.0
|
1.0
|
SG
|
D:CYS58
|
2.3
|
57.6
|
1.0
|
CB
|
D:CYS73
|
2.8
|
53.5
|
1.0
|
CB
|
D:CYS58
|
3.2
|
60.1
|
1.0
|
CB
|
D:CYS76
|
3.4
|
84.7
|
1.0
|
N
|
D:CYS60
|
3.7
|
54.0
|
1.0
|
CB
|
D:CYS60
|
3.7
|
50.8
|
1.0
|
N
|
D:CYS76
|
4.0
|
92.9
|
1.0
|
N
|
D:GLY61
|
4.1
|
73.0
|
1.0
|
N
|
D:LYS62
|
4.1
|
81.1
|
1.0
|
CA
|
D:CYS60
|
4.1
|
54.9
|
1.0
|
N
|
D:ALA59
|
4.2
|
72.4
|
1.0
|
CB
|
D:LYS62
|
4.2
|
77.3
|
1.0
|
CA
|
D:CYS76
|
4.3
|
84.1
|
1.0
|
CA
|
D:CYS73
|
4.3
|
55.0
|
1.0
|
CA
|
D:CYS58
|
4.4
|
60.2
|
1.0
|
C
|
D:CYS58
|
4.5
|
58.6
|
1.0
|
C
|
D:CYS60
|
4.5
|
63.5
|
1.0
|
C
|
D:ALA59
|
4.7
|
80.5
|
1.0
|
CA
|
D:LYS62
|
4.8
|
74.3
|
1.0
|
CD1
|
D:TYR63
|
4.8
|
72.9
|
1.0
|
CB
|
D:ARG75
|
4.8
|
94.0
|
1.0
|
C
|
D:CYS73
|
4.9
|
61.8
|
1.0
|
CA
|
D:ALA59
|
4.9
|
72.3
|
1.0
|
|
Zinc binding site 3 out
of 4 in 5i2d
Go back to
Zinc Binding Sites List in 5i2d
Zinc binding site 3 out
of 4 in the Crystal Structure of T. Thermophilus TTHB099 Class II Transcription Activation Complex: Tap-Rpo
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 3 of Crystal Structure of T. Thermophilus TTHB099 Class II Transcription Activation Complex: Tap-Rpo within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
O:Zn2001
b:53.9
occ:1.00
|
SG
|
O:CYS1112
|
2.3
|
59.7
|
1.0
|
SG
|
O:CYS1194
|
2.3
|
42.1
|
1.0
|
SG
|
O:CYS1201
|
2.3
|
39.3
|
1.0
|
SG
|
O:CYS1204
|
2.3
|
77.4
|
1.0
|
CB
|
O:CYS1194
|
3.2
|
42.7
|
1.0
|
CB
|
O:CYS1204
|
3.2
|
77.1
|
1.0
|
CA
|
O:CYS1194
|
3.4
|
41.8
|
1.0
|
CB
|
O:CYS1112
|
3.5
|
59.2
|
1.0
|
CB
|
O:CYS1201
|
3.6
|
51.2
|
1.0
|
OG1
|
O:THR1196
|
3.7
|
0.5
|
1.0
|
N
|
O:GLN1195
|
3.8
|
58.1
|
1.0
|
C
|
O:CYS1194
|
4.1
|
44.1
|
1.0
|
N
|
O:CYS1112
|
4.2
|
65.1
|
1.0
|
CG2
|
O:THR1114
|
4.2
|
51.2
|
1.0
|
N
|
O:CYS1201
|
4.3
|
49.0
|
1.0
|
CA
|
O:CYS1204
|
4.4
|
78.9
|
1.0
|
N
|
O:CYS1204
|
4.4
|
79.7
|
1.0
|
CA
|
O:CYS1112
|
4.5
|
65.6
|
1.0
|
CA
|
O:CYS1201
|
4.5
|
47.3
|
1.0
|
NH2
|
O:ARG1189
|
4.5
|
88.5
|
1.0
|
N
|
O:THR1196
|
4.6
|
0.8
|
1.0
|
N
|
O:CYS1194
|
4.7
|
37.6
|
1.0
|
CB
|
O:THR1196
|
4.9
|
0.1
|
1.0
|
OD1
|
O:ASP1111
|
5.0
|
84.4
|
1.0
|
|
Zinc binding site 4 out
of 4 in 5i2d
Go back to
Zinc Binding Sites List in 5i2d
Zinc binding site 4 out
of 4 in the Crystal Structure of T. Thermophilus TTHB099 Class II Transcription Activation Complex: Tap-Rpo
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 4 of Crystal Structure of T. Thermophilus TTHB099 Class II Transcription Activation Complex: Tap-Rpo within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
O:Zn2002
b:65.6
occ:1.00
|
SG
|
O:CYS60
|
2.3
|
93.0
|
1.0
|
SG
|
O:CYS73
|
2.3
|
56.6
|
1.0
|
SG
|
O:CYS76
|
2.3
|
0.4
|
1.0
|
SG
|
O:CYS58
|
2.3
|
69.5
|
1.0
|
CB
|
O:CYS73
|
3.1
|
47.4
|
1.0
|
CB
|
O:CYS58
|
3.2
|
72.1
|
1.0
|
CB
|
O:CYS76
|
3.3
|
0.7
|
1.0
|
N
|
O:CYS60
|
3.6
|
96.1
|
1.0
|
CB
|
O:CYS60
|
3.6
|
92.9
|
1.0
|
N
|
O:CYS76
|
3.9
|
0.9
|
1.0
|
N
|
O:GLY61
|
4.0
|
89.5
|
1.0
|
CA
|
O:CYS60
|
4.0
|
96.9
|
1.0
|
N
|
O:LYS62
|
4.1
|
88.2
|
1.0
|
N
|
O:ALA59
|
4.1
|
57.5
|
1.0
|
CA
|
O:CYS76
|
4.2
|
0.1
|
1.0
|
CB
|
O:LYS62
|
4.2
|
84.4
|
1.0
|
C
|
O:CYS60
|
4.4
|
0.6
|
1.0
|
C
|
O:CYS58
|
4.4
|
70.5
|
1.0
|
CA
|
O:CYS58
|
4.4
|
72.1
|
1.0
|
CA
|
O:CYS73
|
4.5
|
48.9
|
1.0
|
C
|
O:ALA59
|
4.6
|
65.6
|
1.0
|
CB
|
O:ARG75
|
4.7
|
89.3
|
1.0
|
CA
|
O:LYS62
|
4.7
|
81.4
|
1.0
|
CA
|
O:ALA59
|
4.8
|
57.4
|
1.0
|
CD1
|
O:TYR63
|
4.9
|
50.0
|
1.0
|
C
|
O:ARG75
|
4.9
|
97.3
|
1.0
|
CA
|
O:GLY61
|
5.0
|
93.4
|
1.0
|
|
Reference:
Y.Feng,
Y.Zhang,
R.H.Ebright.
Structural Basis of Transcription Activation. Science V. 352 1330 2016.
ISSN: ESSN 1095-9203
PubMed: 27284196
DOI: 10.1126/SCIENCE.AAF4417
Page generated: Sun Oct 27 17:47:45 2024
|