Zinc in PDB 5h3j: Crystal Structure of Grasp Domain of GRASP55 Complexed with the GOLGIN45 C-Terminus

The structure of Crystal Structure of Grasp Domain of GRASP55 Complexed with the GOLGIN45 C-Terminus, PDB code: 5h3j was solved by N.Shi, J.Zhao, B.Li, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	31.95 / 1.33
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	97.681, 35.855, 64.030, 90.00, 93.54, 90.00
R / Rfree (%)	13.7 / 16.2

The binding sites of Zinc atom in the Crystal Structure of Grasp Domain of GRASP55 Complexed with the GOLGIN45 C-Terminus (pdb code 5h3j). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the Crystal Structure of Grasp Domain of GRASP55 Complexed with the GOLGIN45 C-Terminus, PDB code: 5h3j:

Zinc binding site 1 out of 1 in the Crystal Structure of Grasp Domain of GRASP55 Complexed with the GOLGIN45 C-Terminus


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Grasp Domain of GRASP55 Complexed with the GOLGIN45 C-Terminus within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn301 b:15.8 occ:1.00 ND1 A:HIS18 2.1 13.4 1.0 SG B:CYS393 2.3 20.3 1.0 SG B:CYS396 2.3 15.6 1.0 SG A:CYS103 2.3 15.9 1.0 HB3 B:CYS393 2.8 25.0 1.0 CE1 A:HIS18 3.0 14.2 1.0 CB B:CYS393 3.1 20.9 1.0 HE1 A:HIS18 3.1 17.1 1.0 H B:CYS396 3.1 26.6 1.0 CG A:HIS18 3.1 11.6 1.0 HB2 A:CYS103 3.2 17.3 1.0 HB2 A:HIS18 3.2 12.9 1.0 HA B:CYS396 3.3 24.2 1.0 N B:CYS396 3.3 22.2 1.0 CB A:CYS103 3.4 14.4 1.0 CB B:CYS396 3.4 18.7 1.0 HB2 B:CYS393 3.4 25.0 1.0 HB3 A:HIS18 3.5 12.9 1.0 CB A:HIS18 3.5 10.8 1.0 CA B:CYS396 3.5 20.2 1.0 HE2 A:PHE39 3.6 13.6 1.0 HB3 A:CYS103 3.7 17.3 1.0 HB2 B:HIS395 3.7 39.0 1.0 HB2 B:CYS396 3.8 22.5 1.0 HD21 A:LEU20 4.0 16.0 1.0 C B:HIS395 4.1 24.5 1.0 H B:HIS395 4.1 36.1 1.0 NE2 A:HIS18 4.1 12.6 1.0 HB3 B:CYS396 4.2 22.5 1.0 CD2 A:HIS18 4.2 12.5 1.0 HZ A:PHE39 4.3 14.5 1.0 CE2 A:PHE39 4.3 11.4 1.0 CA B:CYS393 4.5 23.1 1.0 CB B:HIS395 4.5 32.5 1.0 H A:SER104 4.6 18.9 1.0 O B:CYS393 4.6 27.4 1.0 CA B:HIS395 4.6 29.3 1.0 O B:HOH507 4.6 35.0 1.0 N B:HIS395 4.7 30.1 1.0 O B:HOH510 4.7 18.9 1.0 CZ A:PHE39 4.7 12.1 1.0 C B:CYS393 4.7 25.9 1.0 CA A:CYS103 4.7 13.7 1.0 HB3 B:HIS395 4.8 39.0 1.0 HA A:CYS103 4.8 16.4 1.0 HB2 A:ARG101 4.8 16.1 1.0 O B:HIS395 4.8 25.6 1.0 HD11 A:LEU20 4.9 17.4 1.0 HE2 A:HIS18 4.9 15.1 1.0 CD2 A:LEU20 4.9 13.4 1.0 O A:HOH665 5.0 23.5 1.0 CA A:HIS18 5.0 10.9 1.0

J.Zhao, B.Li, X.Huang, X.Morelli, N.Shi. Structural Basis For the Interaction Between Golgi Reassembly-Stacking Protein GRASP55 and GOLGIN45 J. Biol. Chem. V. 292 2956 2017.
ISSN: ESSN 1083-351X
PubMed: 28049725
DOI: 10.1074/JBC.M116.765990