Zinc in PDB 5cd4: The Type Ie Crispr Cascade Complex From E. Coli, with Two Assemblies in the Asymmetric Unit Arranged Back-to-Back

The structure of The Type Ie Crispr Cascade Complex From E. Coli, with Two Assemblies in the Asymmetric Unit Arranged Back-to-Back, PDB code: 5cd4 was solved by R.N.Jackson, S.M.Golden, J.Carter, B.Wiedenheft, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.74 / 3.20
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	105.990, 244.800, 426.740, 90.00, 90.00, 90.00
R / Rfree (%)	21.2 / 25

The binding sites of Zinc atom in the The Type Ie Crispr Cascade Complex From E. Coli, with Two Assemblies in the Asymmetric Unit Arranged Back-to-Back (pdb code 5cd4). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the The Type Ie Crispr Cascade Complex From E. Coli, with Two Assemblies in the Asymmetric Unit Arranged Back-to-Back, PDB code: 5cd4:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the The Type Ie Crispr Cascade Complex From E. Coli, with Two Assemblies in the Asymmetric Unit Arranged Back-to-Back


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of The Type Ie Crispr Cascade Complex From E. Coli, with Two Assemblies in the Asymmetric Unit Arranged Back-to-Back within 5.0Å range: probe atom residue distance (Å) B Occ I:Zn601 b:0.8 occ:1.00 SG I:CYS253 2.2 47.5 1.0 SG I:CYS140 2.2 89.0 1.0 SG I:CYS250 2.2 0.1 1.0 CB I:CYS253 2.3 48.0 1.0 SG I:CYS143 2.5 77.4 1.0 CB I:CYS250 2.9 76.3 1.0 CB I:CYS140 3.0 78.2 1.0 N I:CYS140 3.5 51.8 1.0 CA I:CYS253 3.5 59.7 1.0 N I:CYS253 3.7 70.0 1.0 CA I:CYS140 3.8 52.4 1.0 CB I:CYS143 3.9 54.9 1.0 CA I:CYS250 4.4 51.9 1.0 C I:CYS253 4.5 58.3 1.0 SG I:CYS252 4.5 98.3 1.0 C I:LEU139 4.6 52.7 1.0 C I:CYS140 4.8 52.8 1.0 N I:GLY254 4.9 67.6 1.0 C I:CYS250 4.9 51.4 1.0 CA I:LEU139 4.9 51.9 1.0 C I:CYS252 4.9 47.9 1.0 N I:CYS143 4.9 61.9 1.0

Zinc binding site 2 out of 2 in the The Type Ie Crispr Cascade Complex From E. Coli, with Two Assemblies in the Asymmetric Unit Arranged Back-to-Back


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of The Type Ie Crispr Cascade Complex From E. Coli, with Two Assemblies in the Asymmetric Unit Arranged Back-to-Back within 5.0Å range: probe atom residue distance (Å) B Occ U:Zn601 b:0.5 occ:1.00 N U:CYS253 1.5 0.9 1.0 SG U:CYS143 2.3 0.9 1.0 CA U:CYS253 2.3 0.7 1.0 C U:CYS252 2.3 0.0 1.0 SG U:CYS253 2.4 83.6 1.0 SG U:CYS250 2.4 87.2 1.0 CB U:CYS253 2.5 92.4 1.0 SG U:CYS140 2.5 0.3 1.0 CA U:CYS252 3.0 0.6 1.0 CB U:CYS252 3.1 0.4 1.0 CB U:CYS143 3.2 97.3 1.0 O U:CYS252 3.2 0.8 1.0 N U:CYS252 3.4 1.0 1.0 C U:CYS253 3.7 0.8 1.0 CB U:CYS250 3.7 80.1 1.0 CB U:CYS140 4.0 79.3 1.0 N U:GLY254 4.1 87.4 1.0 SG U:CYS252 4.2 1.0 1.0 N U:CYS140 4.5 70.2 1.0 C U:SER251 4.5 92.5 1.0 CA U:CYS143 4.6 85.4 1.0 C U:CYS250 4.6 78.1 1.0 O U:CYS250 4.7 84.5 1.0 O U:CYS253 4.7 0.9 1.0 N U:CYS143 4.8 86.3 1.0 CA U:CYS250 4.8 74.5 1.0 CA U:CYS140 4.9 66.3 1.0 N U:SER251 5.0 85.0 1.0

P.B.Van Erp, R.N.Jackson, J.Carter, S.M.Golden, S.Bailey, B.Wiedenheft. Mechanism of Crispr-Rna Guided Recognition of Dna Targets in Escherichia Coli. Nucleic Acids Res. V. 43 8381 2015.
ISSN: ESSN 1362-4962
PubMed: 26243775
DOI: 10.1093/NAR/GKV793