Zinc in PDB 5bwk: 6.0 A Crystal Structure of A GET3-GET4-GET5 Intermediate Complex From S.Cerevisiae

The structure of 6.0 A Crystal Structure of A GET3-GET4-GET5 Intermediate Complex From S.Cerevisiae, PDB code: 5bwk was solved by H.B.Gristick, J.W.Chartron, W.M.Clemons, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 6.00
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	152.430, 127.330, 210.260, 90.00, 110.23, 90.00
R / Rfree (%)	27.4 / 30.3

The binding sites of Zinc atom in the 6.0 A Crystal Structure of A GET3-GET4-GET5 Intermediate Complex From S.Cerevisiae (pdb code 5bwk). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the 6.0 A Crystal Structure of A GET3-GET4-GET5 Intermediate Complex From S.Cerevisiae, PDB code: 5bwk:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in the 6.0 A Crystal Structure of A GET3-GET4-GET5 Intermediate Complex From S.Cerevisiae


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of 6.0 A Crystal Structure of A GET3-GET4-GET5 Intermediate Complex From S.Cerevisiae within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn500 b:0.8 occ:1.00 SG B:CYS285 2.5 0.4 1.0 SG A:CYS285 2.8 0.8 1.0 SG B:CYS288 3.1 0.4 1.0 CB B:CYS288 3.4 0.4 1.0 SG A:CYS288 3.7 0.8 1.0 N B:CYS288 3.8 0.4 1.0 CB A:ARG287 3.9 0.8 1.0 CA B:CYS288 4.0 0.4 1.0 CB B:CYS285 4.1 0.4 1.0 N A:CYS288 4.1 0.8 1.0 CB A:CYS288 4.2 0.8 1.0 CB B:ARG287 4.2 0.4 1.0 C B:ARG287 4.5 0.4 1.0 CB A:CYS285 4.5 0.8 1.0 CA A:CYS288 4.5 0.8 1.0 C A:ARG287 4.6 0.8 1.0 CG A:ARG287 4.7 0.8 1.0 CA A:ARG287 4.9 0.8 1.0 CA B:ARG287 4.9 0.4 1.0

Zinc binding site 2 out of 4 in the 6.0 A Crystal Structure of A GET3-GET4-GET5 Intermediate Complex From S.Cerevisiae


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of 6.0 A Crystal Structure of A GET3-GET4-GET5 Intermediate Complex From S.Cerevisiae within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn501 b:0.6 occ:1.00 SG C:CYS285 2.5 0.6 1.0 SG D:CYS285 2.5 0.2 1.0 SG D:CYS288 3.1 0.2 1.0 CB D:CYS288 3.3 0.2 1.0 SG C:CYS288 3.3 0.6 1.0 CB C:CYS288 3.4 0.6 1.0 N C:CYS288 3.4 0.6 1.0 N D:CYS288 3.6 0.2 1.0 CB C:ARG287 3.7 0.6 1.0 CA C:CYS288 3.8 0.6 1.0 CA D:CYS288 3.8 0.2 1.0 CB D:CYS285 4.1 0.2 1.0 C C:ARG287 4.1 0.6 1.0 CB C:CYS285 4.1 0.6 1.0 CB D:ARG287 4.1 0.2 1.0 C D:ARG287 4.3 0.2 1.0 CA C:ARG287 4.4 0.6 1.0 CG C:ARG287 4.6 0.6 1.0 CA D:ARG287 4.8 0.2 1.0 O C:ARG287 4.8 0.6 1.0 N C:ARG287 4.9 0.6 1.0 O C:CYS285 5.0 0.6 1.0

Zinc binding site 3 out of 4 in the 6.0 A Crystal Structure of A GET3-GET4-GET5 Intermediate Complex From S.Cerevisiae


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of 6.0 A Crystal Structure of A GET3-GET4-GET5 Intermediate Complex From S.Cerevisiae within 5.0Å range: probe atom residue distance (Å) B Occ M:Zn401 b:0.8 occ:1.00 SG M:CYS285 2.5 0.8 1.0 SG N:CYS285 2.6 0.4 1.0 SG N:CYS288 2.7 0.4 1.0 N N:CYS288 2.7 0.4 1.0 CB N:CYS288 2.7 0.4 1.0 CA N:CYS288 3.0 0.4 1.0 C N:ARG287 3.4 0.4 1.0 SG M:CYS288 3.4 0.8 1.0 CB N:ARG287 3.5 0.4 1.0 CB M:CYS288 3.7 0.8 1.0 N M:CYS288 3.8 0.8 1.0 CB M:ARG287 4.0 0.8 1.0 CA N:ARG287 4.0 0.4 1.0 CA M:CYS288 4.1 0.8 1.0 CB M:CYS285 4.1 0.8 1.0 O N:ARG287 4.1 0.4 1.0 CB N:CYS285 4.1 0.4 1.0 C M:ARG287 4.4 0.8 1.0 C N:CYS288 4.5 0.4 1.0 O N:CYS285 4.5 0.4 1.0 N N:ARG287 4.6 0.4 1.0 CG N:ARG287 4.6 0.4 1.0 CA M:ARG287 4.8 0.8 1.0 CG M:ARG287 4.8 0.8 1.0 C N:CYS285 5.0 0.4 1.0

Zinc binding site 4 out of 4 in the 6.0 A Crystal Structure of A GET3-GET4-GET5 Intermediate Complex From S.Cerevisiae


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of 6.0 A Crystal Structure of A GET3-GET4-GET5 Intermediate Complex From S.Cerevisiae within 5.0Å range: probe atom residue distance (Å) B Occ O:Zn401 b:0.9 occ:1.00 SG P:CYS285 2.5 0.8 1.0 SG P:CYS288 2.7 0.8 1.0 SG O:CYS285 2.7 0.9 1.0 CB P:CYS288 2.9 0.8 1.0 N P:CYS288 2.9 0.8 1.0 CA P:CYS288 3.2 0.8 1.0 SG O:CYS288 3.3 0.9 1.0 CB P:ARG287 3.6 0.8 1.0 C P:ARG287 3.6 0.8 1.0 CB O:CYS288 3.8 0.9 1.0 N O:CYS288 4.0 0.9 1.0 CB P:CYS285 4.0 0.8 1.0 CA P:ARG287 4.1 0.8 1.0 CB O:ARG287 4.2 0.9 1.0 CA O:CYS288 4.3 0.9 1.0 CB O:CYS285 4.3 0.9 1.0 O P:ARG287 4.3 0.8 1.0 O P:CYS285 4.5 0.8 1.0 C O:ARG287 4.6 0.9 1.0 CG P:ARG287 4.6 0.8 1.0 N P:ARG287 4.7 0.8 1.0 C P:CYS288 4.7 0.8 1.0 C P:CYS285 5.0 0.8 1.0 CA O:ARG287 5.0 0.9 1.0

H.B.Gristick, M.E.Rome, J.W.Chartron, M.Rao, S.Hess, S.O.Shan, W.M.Clemons. Mechanism of Assembly of A Substrate Transfer Complex During Tail-Anchored Protein Targeting. J.Biol.Chem. V. 290 30006 2015.
ISSN: ESSN 1083-351X
PubMed: 26451041
DOI: 10.1074/JBC.M115.677328