Zinc in PDB 4zyo: Crystal Structure of Human Integral Membrane Stearoyl-Coa Desaturase with Substrate

All present enzymatic activity of Crystal Structure of Human Integral Membrane Stearoyl-Coa Desaturase with Substrate:
1.14.19.1;

The structure of Crystal Structure of Human Integral Membrane Stearoyl-Coa Desaturase with Substrate, PDB code: 4zyo was solved by H.Wang, M.G.Klein, W.Lane, G.Snell, I.Levin, K.Li, H.Zou, B.-C.Sang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	37.52 / 3.25
Space group	P 31 2 1
Cell size a, b, c (Å), α, β, γ (°)	134.108, 134.108, 112.561, 90.00, 90.00, 120.00
R / Rfree (%)	24.1 / 26.4

The binding sites of Zinc atom in the Crystal Structure of Human Integral Membrane Stearoyl-Coa Desaturase with Substrate (pdb code 4zyo). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of Human Integral Membrane Stearoyl-Coa Desaturase with Substrate, PDB code: 4zyo:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of Human Integral Membrane Stearoyl-Coa Desaturase with Substrate


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Human Integral Membrane Stearoyl-Coa Desaturase with Substrate within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn401 b:91.5 occ:1.00 NE2 A:HIS125 2.1 88.7 1.0 NE2 A:HIS157 2.1 0.8 1.0 NE2 A:HIS120 2.1 91.9 1.0 NE2 A:HIS161 2.2 95.2 1.0 NE2 A:HIS301 2.3 0.5 1.0 CD2 A:HIS301 2.8 0.5 1.0 CE1 A:HIS157 2.9 0.8 1.0 CE1 A:HIS125 3.0 94.9 1.0 CD2 A:HIS120 3.0 90.4 1.0 CD2 A:HIS125 3.1 85.4 1.0 CE1 A:HIS120 3.1 91.4 1.0 CE1 A:HIS161 3.1 0.4 1.0 CD2 A:HIS161 3.1 0.8 1.0 CD2 A:HIS157 3.2 98.2 1.0 CE1 A:HIS301 3.5 0.6 1.0 ND1 A:HIS157 4.1 99.3 1.0 ND1 A:HIS125 4.1 94.9 1.0 CG A:HIS301 4.1 0.6 1.0 CG A:HIS125 4.2 87.2 1.0 ND1 A:HIS161 4.2 0.1 1.0 CG A:HIS120 4.2 86.2 1.0 ND1 A:HIS120 4.2 93.9 1.0 CG A:HIS157 4.2 0.1 1.0 CG A:HIS161 4.2 0.8 1.0 C10 A:ST9403 4.3 90.3 1.0 ND1 A:HIS301 4.4 0.1 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of Human Integral Membrane Stearoyl-Coa Desaturase with Substrate


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Human Integral Membrane Stearoyl-Coa Desaturase with Substrate within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn402 b:0.9 occ:1.00 NE2 A:HIS269 2.0 0.2 1.0 NE2 A:HIS160 2.0 0.6 1.0 O A:HOH501 2.1 0.2 1.0 NE2 A:HIS298 2.3 0.7 1.0 NE2 A:HIS302 2.4 0.5 1.0 CE1 A:HIS269 2.6 0.5 1.0 CE1 A:HIS160 2.7 0.7 1.0 CD2 A:HIS298 2.9 0.0 1.0 CD2 A:HIS302 3.2 1.0 1.0 CD2 A:HIS160 3.3 0.1 1.0 CD2 A:HIS269 3.3 0.2 1.0 CE1 A:HIS298 3.4 0.9 1.0 CE1 A:HIS302 3.4 0.1 1.0 ND1 A:HIS269 3.9 0.1 1.0 ND1 A:HIS160 3.9 0.1 1.0 CG A:HIS298 4.1 0.2 1.0 CG A:HIS269 4.2 0.6 1.0 CG A:HIS160 4.2 0.2 1.0 ND1 A:HIS298 4.3 0.6 1.0 CG A:HIS302 4.4 0.5 1.0 OD1 A:ASN265 4.4 99.2 1.0 O A:VAL264 4.4 92.9 1.0 ND1 A:HIS302 4.4 0.6 1.0 CB A:PRO282 4.8 0.2 1.0

H.Wang, M.G.Klein, H.Zou, W.Lane, G.Snell, I.Levin, K.Li, B.C.Sang. Crystal Structure of Human Stearoyl-Coenzyme A Desaturase in Complex with Substrate. Nat.Struct.Mol.Biol. V. 22 581 2015.
ISSN: ESSN 1545-9985
PubMed: 26098317
DOI: 10.1038/NSMB.3049