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Zinc in PDB 4x85: Crystal Structure of Lipase From Geobacillus Stearothermophilus T6 Methanol Stable Variant H86Y/A269T/R374W

Enzymatic activity of Crystal Structure of Lipase From Geobacillus Stearothermophilus T6 Methanol Stable Variant H86Y/A269T/R374W

All present enzymatic activity of Crystal Structure of Lipase From Geobacillus Stearothermophilus T6 Methanol Stable Variant H86Y/A269T/R374W:
3.1.1.3;

Protein crystallography data

The structure of Crystal Structure of Lipase From Geobacillus Stearothermophilus T6 Methanol Stable Variant H86Y/A269T/R374W, PDB code: 4x85 was solved by M.Kanteev, A.Dror, S.Gihaz, A.Fishman, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 24.85 / 2.19
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 49.696, 71.391, 113.564, 90.00, 90.00, 90.00
R / Rfree (%) 20.1 / 23.2

Other elements in 4x85:

The structure of Crystal Structure of Lipase From Geobacillus Stearothermophilus T6 Methanol Stable Variant H86Y/A269T/R374W also contains other interesting chemical elements:

Calcium (Ca) 1 atom

Zinc Binding Sites:

The binding sites of Zinc atom in the Crystal Structure of Lipase From Geobacillus Stearothermophilus T6 Methanol Stable Variant H86Y/A269T/R374W (pdb code 4x85). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the Crystal Structure of Lipase From Geobacillus Stearothermophilus T6 Methanol Stable Variant H86Y/A269T/R374W, PDB code: 4x85:

Zinc binding site 1 out of 1 in 4x85

Go back to Zinc Binding Sites List in 4x85
Zinc binding site 1 out of 1 in the Crystal Structure of Lipase From Geobacillus Stearothermophilus T6 Methanol Stable Variant H86Y/A269T/R374W


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Lipase From Geobacillus Stearothermophilus T6 Methanol Stable Variant H86Y/A269T/R374W within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn401

b:27.9
occ:1.00
 OD1 A:ASP62 2.1 17.8 1.0
NE2 A:HIS82 2.1 23.6 1.0
OD2 A:ASP239 2.1 24.5 1.0
NE2 A:HIS88 2.1 22.4 1.0
OD1 A:ASP239 2.4 21.1 1.0
CG A:ASP239 2.6 23.3 1.0
OD2 A:ASP62 2.6 21.5 1.0
CG A:ASP62 2.7 20.4 1.0
CD2 A:HIS82 3.0 21.3 1.0
CE1 A:HIS82 3.1 20.8 1.0
CD2 A:HIS88 3.1 20.5 1.0
CE1 A:HIS88 3.1 22.2 1.0
O A:HOH601 3.9 24.4 1.0
OG A:SER59 3.9 16.8 1.0
CB A:ASP239 4.1 21.8 1.0
CB A:ASP62 4.2 17.6 1.0
ND1 A:HIS82 4.2 19.1 1.0
CG A:HIS82 4.2 20.5 1.0
ND1 A:HIS88 4.2 21.1 1.0
CG A:HIS88 4.3 20.7 1.0
CD1 A:TRP61 4.5 15.3 1.0
O A:ASP239 4.7 22.9 1.0
N A:ASP62 4.7 17.2 1.0
CA A:ASP62 4.8 17.8 1.0
CD2 A:TYR86 4.8 22.8 1.0

Reference:

A.Dror, M.Kanteev, I.Kagan, S.Gihaz, A.Shahar, A.Fishman. Structural Insights Into Methanol-Stable Variants of Lipase T6 From Geobacillus Stearothermophilus. Appl.Microbiol.Biotechnol. V. 99 9449 2015.
ISSN: ESSN 1432-0614
PubMed: 26026940
DOI: 10.1007/S00253-015-6700-4
Page generated: Wed Aug 20 23:19:24 2025

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