Atomistry » Zinc » PDB 4o6p-4ojo » 4oio
Atomistry »
  Zinc »
    PDB 4o6p-4ojo »
      4oio »

Zinc in PDB 4oio: Crystal Structure of Thermus Thermophilus Pre-Insertion Substrate Complex For De Novo Transcription Initiation

Enzymatic activity of Crystal Structure of Thermus Thermophilus Pre-Insertion Substrate Complex For De Novo Transcription Initiation

All present enzymatic activity of Crystal Structure of Thermus Thermophilus Pre-Insertion Substrate Complex For De Novo Transcription Initiation:
2.7.7.6;

Protein crystallography data

The structure of Crystal Structure of Thermus Thermophilus Pre-Insertion Substrate Complex For De Novo Transcription Initiation, PDB code: 4oio was solved by Y.Zhang, R.H.Ebright, E.Arnold, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 48.92 / 3.10
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 184.110, 103.780, 296.090, 90.00, 98.86, 90.00
R / Rfree (%) 20.5 / 25.6

Other elements in 4oio:

The structure of Crystal Structure of Thermus Thermophilus Pre-Insertion Substrate Complex For De Novo Transcription Initiation also contains other interesting chemical elements:

Magnesium (Mg) 2 atoms

Zinc Binding Sites:

The binding sites of Zinc atom in the Crystal Structure of Thermus Thermophilus Pre-Insertion Substrate Complex For De Novo Transcription Initiation (pdb code 4oio). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of Thermus Thermophilus Pre-Insertion Substrate Complex For De Novo Transcription Initiation, PDB code: 4oio:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in 4oio

Go back to Zinc Binding Sites List in 4oio
Zinc binding site 1 out of 2 in the Crystal Structure of Thermus Thermophilus Pre-Insertion Substrate Complex For De Novo Transcription Initiation


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Thermus Thermophilus Pre-Insertion Substrate Complex For De Novo Transcription Initiation within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Zn2001

b:20.4
occ:1.00
SG D:CYS1204 2.2 11.2 1.0
SG D:CYS1194 2.2 10.5 1.0
SG D:CYS1112 2.2 15.5 1.0
SG D:CYS1201 2.4 11.0 1.0
CB D:CYS1112 3.3 19.8 1.0
CB D:CYS1194 3.3 17.5 1.0
CB D:CYS1201 3.4 14.2 1.0
CB D:CYS1204 3.4 13.2 1.0
CA D:CYS1194 3.7 16.0 1.0
OG1 D:THR1196 3.8 24.1 1.0
NH2 D:ARG1189 3.9 17.5 1.0
N D:CYS1112 4.0 19.3 1.0
N D:GLN1195 4.0 17.9 1.0
N D:CYS1201 4.0 13.0 1.0
CA D:CYS1112 4.2 16.3 1.0
CA D:CYS1201 4.3 12.9 1.0
C D:CYS1194 4.3 17.4 1.0
CG2 D:THR1114 4.4 22.0 1.0
N D:CYS1204 4.5 17.3 1.0
CA D:CYS1204 4.5 15.7 1.0
N D:THR1196 4.5 15.7 1.0
CB D:THR1196 4.8 21.5 1.0
N D:CYS1194 4.9 12.0 1.0
C D:CYS1201 4.9 11.1 1.0
CZ D:ARG1189 5.0 22.3 1.0

Zinc binding site 2 out of 2 in 4oio

Go back to Zinc Binding Sites List in 4oio
Zinc binding site 2 out of 2 in the Crystal Structure of Thermus Thermophilus Pre-Insertion Substrate Complex For De Novo Transcription Initiation


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Thermus Thermophilus Pre-Insertion Substrate Complex For De Novo Transcription Initiation within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Zn2002

b:58.0
occ:1.00
SG D:CYS76 2.3 46.2 1.0
SG D:CYS73 2.3 63.2 1.0
SG D:CYS60 2.3 42.3 1.0
SG D:CYS58 2.4 65.2 1.0
CB D:CYS73 2.8 58.5 1.0
CB D:CYS58 3.2 47.1 1.0
CB D:CYS76 3.8 48.0 1.0
CB D:CYS60 3.9 47.5 1.0
N D:CYS60 3.9 55.3 1.0
CB D:LYS62 4.0 59.5 1.0
N D:LYS62 4.1 45.1 1.0
N D:CYS76 4.2 65.5 1.0
N D:GLY61 4.3 56.6 1.0
N D:ALA59 4.3 36.4 1.0
CD2 D:TYR63 4.3 60.5 1.0
CA D:CYS73 4.3 52.6 1.0
CA D:CYS60 4.4 51.6 1.0
CA D:CYS58 4.4 45.6 1.0
C D:CYS58 4.5 38.6 1.0
CA D:CYS76 4.6 60.8 1.0
CA D:LYS62 4.6 56.6 1.0
CE2 D:TYR63 4.6 69.1 1.0
C D:CYS60 4.7 54.9 1.0
N D:TYR63 4.7 62.6 1.0
C D:ALA59 4.9 56.2 1.0

Reference:

Y.Zhang, D.Degen, M.X.Ho, E.Sineva, K.Y.Ebright, Y.W.Ebright, V.Mekler, H.Vahedian-Movahed, Y.Feng, R.Yin, S.Tuske, H.Irschik, R.Jansen, S.Maffioli, S.Donadio, E.Arnold, R.H.Ebright. GE23077 Binds to the Rna Polymerase 'I' and 'I+1' Sites and Prevents the Binding of Initiating Nucleotides. Elife V. 3 02450 2014.
ISSN: ESSN 2050-084X
PubMed: 24755292
Page generated: Wed Dec 16 05:38:47 2020

Last articles

Zn in 8WB0
Zn in 8WAX
Zn in 8WAU
Zn in 8WAZ
Zn in 8WAY
Zn in 8WAV
Zn in 8WAW
Zn in 8WAT
Zn in 8W7M
Zn in 8WD3
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy