Zinc in PDB 4kjg: Structure of Rat Intestinal Alkaline Phosphatase Expressed in Insect Cell

Enzymatic activity of Structure of Rat Intestinal Alkaline Phosphatase Expressed in Insect Cell

All present enzymatic activity of Structure of Rat Intestinal Alkaline Phosphatase Expressed in Insect Cell:
3.1.3.1;

Protein crystallography data

The structure of Structure of Rat Intestinal Alkaline Phosphatase Expressed in Insect Cell, PDB code: 4kjg was solved by K.Ghosh, R.K.Anumula, B.K.Laksmaiah, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 47.42 / 2.38
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 90.306, 167.170, 71.843, 90.00, 90.00, 90.00
R / Rfree (%) 16.4 / 20.4

Other elements in 4kjg:

The structure of Structure of Rat Intestinal Alkaline Phosphatase Expressed in Insect Cell also contains other interesting chemical elements:

Magnesium (Mg) 6 atoms

Zinc Binding Sites:

The binding sites of Zinc atom in the Structure of Rat Intestinal Alkaline Phosphatase Expressed in Insect Cell (pdb code 4kjg). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the Structure of Rat Intestinal Alkaline Phosphatase Expressed in Insect Cell, PDB code: 4kjg:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in 4kjg

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Zinc binding site 1 out of 4 in the Structure of Rat Intestinal Alkaline Phosphatase Expressed in Insect Cell


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Structure of Rat Intestinal Alkaline Phosphatase Expressed in Insect Cell within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn1001

b:24.1
occ:1.00
 NE2 A:HIS320 2.0 19.1 1.0
NE2 A:HIS432 2.1 18.7 1.0
OD2 A:ASP316 2.1 22.3 1.0
O5 A:4NP1006 2.2 34.4 1.0
CG A:ASP316 2.8 20.0 1.0
OD1 A:ASP316 2.8 21.1 1.0
CE1 A:HIS320 3.0 18.6 1.0
CE1 A:HIS432 3.0 18.0 1.0
CD2 A:HIS320 3.0 19.3 1.0
CD2 A:HIS432 3.1 19.4 1.0
N A:4NP1006 3.1 42.3 1.0
C4 A:4NP1006 3.3 51.0 1.0
C5 A:4NP1006 3.6 52.9 1.0
O A:HOH1111 3.8 37.0 1.0
OG A:SER92 3.9 27.8 1.0
C3 A:4NP1006 4.0 56.0 1.0
CE1 A:HIS358 4.1 17.4 1.0
CG A:HIS320 4.2 18.3 1.0
ND1 A:HIS320 4.2 19.5 1.0
ND1 A:HIS432 4.2 19.3 1.0
ZN A:ZN1002 4.2 34.2 0.8
CG A:HIS432 4.2 18.6 1.0
NE2 A:HIS358 4.2 17.5 1.0
O6 A:4NP1006 4.2 41.1 1.0
CB A:ASP316 4.2 17.0 1.0
C6 A:4NP1006 4.4 56.0 1.0
CE1 A:HIS360 4.6 16.6 1.0
OD1 A:ASP42 4.7 27.0 1.0
NE2 A:HIS360 4.8 16.8 1.0
C2 A:4NP1006 4.8 59.1 1.0
OH B:TYR367 4.9 18.7 1.0
C1 A:4NP1006 4.9 59.8 1.0

Zinc binding site 2 out of 4 in 4kjg

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Zinc binding site 2 out of 4 in the Structure of Rat Intestinal Alkaline Phosphatase Expressed in Insect Cell


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Structure of Rat Intestinal Alkaline Phosphatase Expressed in Insect Cell within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn1002

b:34.2
occ:0.75
 OD1 A:ASP42 2.0 27.0 1.0
OD2 A:ASP357 2.1 24.4 1.0
NE2 A:HIS358 2.1 17.5 1.0
OG A:SER92 2.3 27.8 1.0
CG A:ASP42 2.8 27.9 1.0
CG A:ASP357 2.9 22.1 1.0
CE1 A:HIS358 3.1 17.4 1.0
CD2 A:HIS358 3.1 17.1 1.0
OD2 A:ASP42 3.1 38.3 1.0
OD1 A:ASP357 3.2 19.8 1.0
CB A:SER92 3.3 18.9 1.0
O5 A:4NP1006 3.7 34.4 1.0
CA A:SER92 3.8 17.9 1.0
OD1 A:ASP316 3.8 21.1 1.0
CG A:ASP316 3.9 20.0 1.0
OD2 A:ASP316 4.0 22.3 1.0
CG A:HIS358 4.1 15.5 1.0
ND1 A:HIS358 4.2 18.1 1.0
ZN A:ZN1001 4.2 24.1 1.0
CB A:ASP42 4.2 20.7 1.0
O A:HOH1109 4.2 15.4 1.0
N A:GLY43 4.2 16.9 1.0
N A:SER92 4.3 19.5 1.0
CE1 A:HIS432 4.3 18.0 1.0
CB A:ASP357 4.3 16.0 1.0
NE2 A:HIS432 4.3 18.7 1.0
MG A:MG1003 4.3 54.4 1.0
CA A:ASP42 4.5 19.5 1.0
CB A:ASP316 4.6 17.0 1.0
C A:ASP42 4.6 21.7 1.0
N A:4NP1006 4.8 42.3 1.0
OG A:SER155 4.8 30.1 1.0
CA A:GLY43 4.9 16.7 1.0
C A:ASP91 5.0 22.6 1.0
O A:HOH1266 5.0 45.1 1.0

Zinc binding site 3 out of 4 in 4kjg

Go back to Zinc Binding Sites List in 4kjg
Zinc binding site 3 out of 4 in the Structure of Rat Intestinal Alkaline Phosphatase Expressed in Insect Cell


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Structure of Rat Intestinal Alkaline Phosphatase Expressed in Insect Cell within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn1001

b:25.7
occ:1.00
 NE2 B:HIS320 2.0 19.4 1.0
NE2 B:HIS432 2.1 15.7 1.0
OD2 B:ASP316 2.1 23.4 1.0
O5 B:4NP1006 2.3 31.8 1.0
CG B:ASP316 2.8 21.4 1.0
OD1 B:ASP316 2.8 23.5 1.0
C5 B:4NP1006 2.9 45.3 1.0
CE1 B:HIS320 3.0 18.4 1.0
CE1 B:HIS432 3.0 15.1 1.0
CD2 B:HIS320 3.0 19.6 1.0
CD2 B:HIS432 3.1 15.9 1.0
N B:4NP1006 3.1 38.4 1.0
C4 B:4NP1006 3.2 42.7 1.0
C6 B:4NP1006 3.7 48.8 1.0
OG B:SER92 3.9 26.9 1.0
O B:HOH1113 4.0 31.1 1.0
CE1 B:HIS358 4.1 13.1 1.0
ND1 B:HIS320 4.2 18.8 1.0
CG B:HIS320 4.2 17.0 1.0
ZN B:ZN1002 4.2 32.5 0.8
ND1 B:HIS432 4.2 16.3 1.0
NE2 B:HIS358 4.2 12.8 1.0
CG B:HIS432 4.2 14.9 1.0
CB B:ASP316 4.2 17.2 1.0
C3 B:4NP1006 4.3 44.9 1.0
O6 B:4NP1006 4.3 38.8 1.0
CE1 B:HIS360 4.6 20.9 1.0
C1 B:4NP1006 4.6 50.5 1.0
OD1 B:ASP42 4.7 26.7 1.0
NE2 B:HIS360 4.8 21.5 1.0
OH A:TYR367 4.9 25.1 1.0
C2 B:4NP1006 5.0 47.7 1.0

Zinc binding site 4 out of 4 in 4kjg

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Zinc binding site 4 out of 4 in the Structure of Rat Intestinal Alkaline Phosphatase Expressed in Insect Cell


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Structure of Rat Intestinal Alkaline Phosphatase Expressed in Insect Cell within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn1002

b:32.5
occ:0.75
 OD1 B:ASP42 2.0 26.7 1.0
OD2 B:ASP357 2.1 22.4 1.0
NE2 B:HIS358 2.2 12.8 1.0
OG B:SER92 2.3 26.9 1.0
CG B:ASP42 2.8 29.0 1.0
CG B:ASP357 2.9 18.6 1.0
CE1 B:HIS358 3.1 13.1 1.0
CD2 B:HIS358 3.1 12.8 1.0
OD2 B:ASP42 3.1 40.1 1.0
OD1 B:ASP357 3.2 17.5 1.0
CB B:SER92 3.3 19.7 1.0
CA B:SER92 3.7 17.4 1.0
O5 B:4NP1006 3.7 31.8 1.0
OD1 B:ASP316 3.8 23.5 1.0
CG B:ASP316 3.9 21.4 1.0
OD2 B:ASP316 4.0 23.4 1.0
CG B:HIS358 4.2 12.7 1.0
ZN B:ZN1001 4.2 25.7 1.0
ND1 B:HIS358 4.2 14.1 1.0
CB B:ASP42 4.2 18.3 1.0
N B:GLY43 4.2 16.0 1.0
N B:SER92 4.2 18.7 1.0
O B:HOH1107 4.2 17.0 1.0
CB B:ASP357 4.3 12.9 1.0
CE1 B:HIS432 4.3 15.1 1.0
NE2 B:HIS432 4.3 15.7 1.0
MG B:MG1003 4.5 41.4 1.0
CA B:ASP42 4.5 16.5 1.0
CB B:ASP316 4.6 17.2 1.0
C B:ASP42 4.6 19.7 1.0
O B:HOH1112 4.8 43.7 1.0
OG B:SER155 4.8 24.7 1.0
N B:4NP1006 4.8 38.4 1.0
O B:HOH1113 4.9 31.1 1.0
CA B:GLY43 4.9 15.5 1.0
C B:ASP91 5.0 25.0 1.0

Reference:

K.Ghosh, D.Mazumder Tagore, R.Anumula, B.Lakshmaiah, P.P.Kumar, S.Singaram, T.Matan, S.Kallipatti, S.Selvam, P.Krishnamurthy, M.Ramarao. Crystal Structure of Rat Intestinal Alkaline Phosphatase - Role of Crown Domain in Mammalian Alkaline Phosphatases. J.Struct.Biol. V. 184 182 2013.
ISSN: ISSN 1047-8477
PubMed: 24076154
DOI: 10.1016/J.JSB.2013.09.017
Page generated: Wed Dec 16 05:29:20 2020

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