Zinc in PDB 4jkr: Crystal Structure of E. Coli Rna Polymerase in Complex with Ppgpp
Enzymatic activity of Crystal Structure of E. Coli Rna Polymerase in Complex with Ppgpp
All present enzymatic activity of Crystal Structure of E. Coli Rna Polymerase in Complex with Ppgpp:
2.7.7.6;
Protein crystallography data
The structure of Crystal Structure of E. Coli Rna Polymerase in Complex with Ppgpp, PDB code: 4jkr
was solved by
Y.Zuo,
Y.Wang,
T.A.Steitz,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
39.97 /
4.20
|
Space group
|
P 21 21 21
|
Cell size a, b, c (Å), α, β, γ (°)
|
187.575,
206.164,
311.062,
90.00,
90.00,
90.00
|
R / Rfree (%)
|
24.7 /
31.8
|
Other elements in 4jkr:
The structure of Crystal Structure of E. Coli Rna Polymerase in Complex with Ppgpp also contains other interesting chemical elements:
Zinc Binding Sites:
The binding sites of Zinc atom in the Crystal Structure of E. Coli Rna Polymerase in Complex with Ppgpp
(pdb code 4jkr). This binding sites where shown within
5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the
Crystal Structure of E. Coli Rna Polymerase in Complex with Ppgpp, PDB code: 4jkr:
Jump to Zinc binding site number:
1;
2;
3;
4;
Zinc binding site 1 out
of 4 in 4jkr
Go back to
Zinc Binding Sites List in 4jkr
Zinc binding site 1 out
of 4 in the Crystal Structure of E. Coli Rna Polymerase in Complex with Ppgpp
 Mono view
 Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 1 of Crystal Structure of E. Coli Rna Polymerase in Complex with Ppgpp within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:Zn2002
b:0.8
occ:1.00
|
SG
|
D:CYS72
|
2.3
|
0.1
|
1.0
|
SG
|
D:CYS70
|
2.4
|
0.7
|
1.0
|
SG
|
D:CYS88
|
2.5
|
0.2
|
1.0
|
CB
|
D:CYS88
|
3.3
|
0.9
|
1.0
|
N
|
D:CYS72
|
3.4
|
0.5
|
1.0
|
CB
|
D:CYS70
|
3.4
|
0.9
|
1.0
|
CB
|
D:CYS72
|
3.4
|
0.8
|
1.0
|
SG
|
D:CYS85
|
3.8
|
0.6
|
1.0
|
N
|
D:LEU71
|
3.8
|
0.8
|
1.0
|
CA
|
D:CYS72
|
4.0
|
0.4
|
1.0
|
C
|
D:LEU71
|
4.4
|
0.7
|
1.0
|
N
|
D:CYS88
|
4.4
|
0.0
|
1.0
|
C
|
D:CYS70
|
4.4
|
0.8
|
1.0
|
CA
|
D:CYS88
|
4.5
|
0.4
|
1.0
|
CA
|
D:CYS70
|
4.5
|
0.2
|
1.0
|
CA
|
D:LEU71
|
4.5
|
0.6
|
1.0
|
N
|
D:GLY73
|
4.6
|
0.7
|
1.0
|
CB
|
D:LEU71
|
4.7
|
0.1
|
1.0
|
C
|
D:CYS72
|
4.8
|
0.5
|
1.0
|
CB
|
D:LYS87
|
4.9
|
0.1
|
1.0
|
CB
|
D:CYS85
|
4.9
|
0.1
|
1.0
|
CD2
|
D:TYR75
|
4.9
|
0.9
|
1.0
|
|
Zinc binding site 2 out
of 4 in 4jkr
Go back to
Zinc Binding Sites List in 4jkr
Zinc binding site 2 out
of 4 in the Crystal Structure of E. Coli Rna Polymerase in Complex with Ppgpp
 Mono view
 Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 2 of Crystal Structure of E. Coli Rna Polymerase in Complex with Ppgpp within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:Zn2003
b:0.7
occ:1.00
|
SG
|
D:CYS888
|
1.7
|
0.4
|
1.0
|
SG
|
D:CYS895
|
1.9
|
0.2
|
1.0
|
SG
|
D:CYS898
|
2.0
|
0.7
|
1.0
|
SG
|
D:CYS814
|
2.1
|
1.0
|
1.0
|
CB
|
D:CYS888
|
3.0
|
0.9
|
1.0
|
CB
|
D:CYS895
|
3.3
|
0.1
|
1.0
|
CB
|
D:CYS814
|
3.3
|
0.6
|
1.0
|
CA
|
D:CYS888
|
3.4
|
0.2
|
1.0
|
CB
|
D:CYS898
|
3.6
|
0.8
|
1.0
|
O
|
D:SER887
|
3.7
|
0.9
|
1.0
|
O
|
D:CYS898
|
3.8
|
0.1
|
1.0
|
OG1
|
D:THR890
|
4.0
|
0.4
|
1.0
|
N
|
D:CYS895
|
4.3
|
0.2
|
1.0
|
N
|
D:CYS888
|
4.3
|
0.3
|
1.0
|
C
|
D:SER887
|
4.4
|
0.8
|
1.0
|
CA
|
D:CYS895
|
4.4
|
0.3
|
1.0
|
C
|
D:CYS888
|
4.4
|
0.9
|
1.0
|
N
|
D:ASP889
|
4.5
|
0.4
|
1.0
|
O
|
D:SER884
|
4.5
|
0.0
|
1.0
|
C
|
D:CYS898
|
4.6
|
0.3
|
1.0
|
CA
|
D:CYS898
|
4.7
|
0.8
|
1.0
|
CA
|
D:CYS814
|
4.7
|
0.0
|
1.0
|
OG1
|
D:THR816
|
4.7
|
0.4
|
1.0
|
CB
|
D:THR890
|
5.0
|
0.5
|
1.0
|
|
Zinc binding site 3 out
of 4 in 4jkr
Go back to
Zinc Binding Sites List in 4jkr
Zinc binding site 3 out
of 4 in the Crystal Structure of E. Coli Rna Polymerase in Complex with Ppgpp
 Mono view
 Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 3 of Crystal Structure of E. Coli Rna Polymerase in Complex with Ppgpp within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
J:Zn1501
b:0.1
occ:1.00
|
SG
|
J:CYS70
|
2.3
|
0.7
|
1.0
|
SG
|
J:CYS72
|
2.3
|
0.7
|
1.0
|
N
|
J:CYS72
|
3.2
|
0.4
|
1.0
|
SG
|
J:CYS88
|
3.3
|
0.1
|
1.0
|
CB
|
J:CYS72
|
3.4
|
0.7
|
1.0
|
CB
|
J:CYS70
|
3.4
|
0.6
|
1.0
|
SG
|
J:CYS85
|
3.4
|
0.1
|
1.0
|
CB
|
J:CYS88
|
3.6
|
0.0
|
1.0
|
N
|
J:LEU71
|
3.7
|
0.0
|
1.0
|
CA
|
J:CYS72
|
3.8
|
0.5
|
1.0
|
N
|
J:GLY73
|
4.2
|
0.9
|
1.0
|
C
|
J:LEU71
|
4.2
|
0.7
|
1.0
|
CA
|
J:LEU71
|
4.3
|
0.5
|
1.0
|
C
|
J:CYS70
|
4.3
|
0.9
|
1.0
|
CB
|
J:CYS85
|
4.3
|
0.9
|
1.0
|
N
|
J:CYS88
|
4.4
|
0.5
|
1.0
|
CA
|
J:CYS70
|
4.4
|
0.1
|
1.0
|
CB
|
J:LEU71
|
4.4
|
0.4
|
1.0
|
C
|
J:CYS72
|
4.5
|
0.2
|
1.0
|
CA
|
J:CYS88
|
4.6
|
0.2
|
1.0
|
N
|
J:LYS74
|
4.8
|
0.5
|
1.0
|
CB
|
J:LYS87
|
4.9
|
0.5
|
1.0
|
CB
|
J:LYS74
|
5.0
|
0.4
|
1.0
|
|
Zinc binding site 4 out
of 4 in 4jkr
Go back to
Zinc Binding Sites List in 4jkr
Zinc binding site 4 out
of 4 in the Crystal Structure of E. Coli Rna Polymerase in Complex with Ppgpp
 Mono view
 Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 4 of Crystal Structure of E. Coli Rna Polymerase in Complex with Ppgpp within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
J:Zn1502
b:0.6
occ:1.00
|
SG
|
J:CYS888
|
2.1
|
0.4
|
1.0
|
SG
|
J:CYS814
|
2.1
|
0.1
|
1.0
|
SG
|
J:CYS898
|
2.7
|
0.9
|
1.0
|
SG
|
J:CYS895
|
2.9
|
0.2
|
1.0
|
OG1
|
J:THR890
|
3.1
|
0.9
|
1.0
|
N
|
J:CYS895
|
3.2
|
0.8
|
1.0
|
CB
|
J:CYS814
|
3.4
|
0.2
|
1.0
|
CB
|
J:CYS895
|
3.4
|
0.0
|
1.0
|
CB
|
J:CYS888
|
3.6
|
0.3
|
1.0
|
CB
|
J:CYS898
|
3.7
|
0.3
|
1.0
|
CA
|
J:CYS895
|
3.8
|
0.5
|
1.0
|
CA
|
J:CYS888
|
4.0
|
0.7
|
1.0
|
CB
|
J:THR890
|
4.0
|
0.8
|
1.0
|
N
|
J:ASP889
|
4.1
|
0.4
|
1.0
|
C
|
J:VAL894
|
4.1
|
0.4
|
1.0
|
N
|
J:THR890
|
4.2
|
0.3
|
1.0
|
CA
|
J:CYS814
|
4.2
|
1.0
|
1.0
|
CA
|
J:VAL894
|
4.2
|
0.8
|
1.0
|
C
|
J:CYS888
|
4.6
|
0.1
|
1.0
|
N
|
J:CYS814
|
4.7
|
0.1
|
1.0
|
CA
|
J:THR890
|
4.7
|
0.6
|
1.0
|
CG1
|
J:VAL894
|
4.8
|
0.1
|
1.0
|
O
|
J:GLY893
|
5.0
|
0.5
|
1.0
|
|
Reference:
Y.Zuo,
Y.Wang,
T.A.Steitz.
The Mechanism of E. Coli Rna Polymerase Regulation By Ppgpp Is Suggested By the Structure of Their Complex. Mol.Cell V. 50 430 2013.
ISSN: ISSN 1097-2765
PubMed: 23623685
DOI: 10.1016/J.MOLCEL.2013.03.020
Page generated: Sun Oct 27 01:19:50 2024
|