Zinc in PDB 4iao: Crystal Structure of SIR2 C543S Mutant in Complex with Sid Domain of SIR4

The structure of Crystal Structure of SIR2 C543S Mutant in Complex with Sid Domain of SIR4, PDB code: 4iao was solved by H.C.Hsu, C.L.Wang, M.Wang, N.Yang, Z.Chen, R.Sternglanz, R.M.Xu, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	36.43 / 2.90
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	166.406, 178.752, 121.564, 90.00, 90.00, 90.00
R / Rfree (%)	22.7 / 26.9

The binding sites of Zinc atom in the Crystal Structure of SIR2 C543S Mutant in Complex with Sid Domain of SIR4 (pdb code 4iao). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of SIR2 C543S Mutant in Complex with Sid Domain of SIR4, PDB code: 4iao:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of SIR2 C543S Mutant in Complex with Sid Domain of SIR4


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of SIR2 C543S Mutant in Complex with Sid Domain of SIR4 within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn601 b:57.3 occ:1.00 SG A:CYS372 2.4 57.1 1.0 SG A:CYS396 2.5 55.1 1.0 SG A:CYS399 2.6 52.8 1.0 SG A:CYS375 2.7 64.3 1.0 CB A:CYS375 3.1 47.6 1.0 CB A:CYS396 3.1 60.9 1.0 CB A:CYS372 3.2 47.1 1.0 CB A:CYS399 3.5 55.5 1.0 OG1 A:THR374 3.7 72.2 1.0 N A:CYS375 3.8 64.9 1.0 N A:CYS399 4.0 64.9 1.0 CA A:CYS375 4.0 52.7 1.0 CA A:CYS399 4.3 61.7 1.0 CA A:CYS396 4.6 58.8 1.0 CA A:CYS372 4.7 49.9 1.0 C A:CYS375 4.7 64.4 1.0 C A:TYR398 4.8 75.7 1.0 CA A:GLY436 4.8 48.6 1.0 CB A:TYR398 4.8 76.6 1.0 N A:HIS376 5.0 73.2 1.0 C A:THR374 5.0 66.6 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of SIR2 C543S Mutant in Complex with Sid Domain of SIR4


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of SIR2 C543S Mutant in Complex with Sid Domain of SIR4 within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn601 b:52.9 occ:1.00 SG B:CYS372 2.5 53.9 1.0 SG B:CYS396 2.5 56.1 1.0 SG B:CYS399 2.6 53.8 1.0 SG B:CYS375 2.7 64.2 1.0 CB B:CYS375 3.0 45.5 1.0 CB B:CYS396 3.1 59.8 1.0 CB B:CYS372 3.1 47.5 1.0 CB B:CYS399 3.5 55.0 1.0 N B:CYS375 3.7 58.9 1.0 OG1 B:THR374 3.7 70.2 1.0 CA B:CYS375 3.9 51.0 1.0 N B:CYS399 4.0 62.1 1.0 CA B:CYS399 4.4 58.7 1.0 CD2 B:TYR398 4.4 92.9 1.0 CA B:CYS396 4.6 62.5 1.0 CA B:CYS372 4.6 51.6 1.0 C B:CYS375 4.6 64.4 1.0 CB B:TYR398 4.8 85.2 1.0 CA B:GLY436 4.9 45.3 1.0 C B:THR374 4.9 64.5 1.0 C B:TYR398 4.9 70.0 1.0 N B:HIS376 4.9 68.5 1.0 CB B:THR374 5.0 59.5 1.0

H.C.Hsu, C.L.Wang, M.Wang, N.Yang, Z.Chen, R.Sternglanz, R.M.Xu. Structural Basis For Allosteric Stimulation of SIR2 Activity By SIR4 Binding Genes Dev. V. 27 64 2013.
ISSN: ISSN 0890-9369
PubMed: 23307867
DOI: 10.1101/GAD.208140.112