Zinc in PDB 4gwc: Crystal Structure of MN2+2,ZN2+-Human Arginase I

All present enzymatic activity of Crystal Structure of MN2+2,ZN2+-Human Arginase I:
3.5.3.1;

The structure of Crystal Structure of MN2+2,ZN2+-Human Arginase I, PDB code: 4gwc was solved by E.L.D'antonio, Y.Hai, D.W.Christianson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 1.90
Space group	P 3
Cell size a, b, c (Å), α, β, γ (°)	90.829, 90.829, 70.068, 90.00, 90.00, 120.00
R / Rfree (%)	15.4 / 22.1

The structure of Crystal Structure of MN2+2,ZN2+-Human Arginase I also contains other interesting chemical elements:

Manganese

(Mn)

4 atoms

The binding sites of Zinc atom in the Crystal Structure of MN2+2,ZN2+-Human Arginase I (pdb code 4gwc). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of MN2+2,ZN2+-Human Arginase I, PDB code: 4gwc:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of MN2+2,ZN2+-Human Arginase I


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of MN2+2,ZN2+-Human Arginase I within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn403 b:41.6 occ:0.60 NE2 A:HIS141 2.2 22.8 1.0 O A:HOH543 2.6 45.4 1.0 CD2 A:HIS141 3.0 23.8 1.0 CE1 A:HIS141 3.2 23.1 1.0 O A:HOH505 3.2 41.4 1.0 O A:HOH502 3.8 40.8 1.0 ND2 A:ASN139 4.1 28.7 1.0 CG2 A:THR246 4.1 32.5 1.0 CG A:HIS141 4.2 24.1 1.0 ND1 A:HIS141 4.2 23.9 1.0 CB A:ASN139 4.5 29.9 1.0 OG1 A:THR246 4.8 35.6 1.0 CG A:ASN139 4.8 30.4 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of MN2+2,ZN2+-Human Arginase I


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of MN2+2,ZN2+-Human Arginase I within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn403 b:36.5 occ:0.35 NE2 B:HIS141 2.2 35.5 1.0 CE1 B:HIS141 3.0 36.9 1.0 O B:HOH511 3.1 30.7 1.0 CD2 B:HIS141 3.4 37.0 1.0 OG1 B:THR246 3.7 32.9 1.0 ND2 B:ASN139 3.9 32.5 1.0 ND1 B:HIS141 4.2 35.0 1.0 CG B:HIS141 4.4 35.7 1.0 CB B:ASN139 4.7 36.5 1.0 CG B:ASN139 4.8 33.8 1.0 CG2 B:THR246 4.8 31.2 1.0 CB B:THR246 4.9 31.7 1.0 NH2 B:ARG21 5.0 43.0 1.0

E.L.D'antonio, Y.Hai, D.W.Christianson. Structure and Function of Non-Native Metal Clusters in Human Arginase I. Biochemistry V. 51 8399 2012.
ISSN: ISSN 0006-2960
PubMed: 23061982
DOI: 10.1021/BI301145N