Zinc in PDB 4giz: Crystal Structure of Full-Length Human Papillomavirus Oncoprotein E6 in Complex with Lxxll Peptide of Ubiquitin Ligase E6AP at 2.55 A Resolution

The structure of Crystal Structure of Full-Length Human Papillomavirus Oncoprotein E6 in Complex with Lxxll Peptide of Ubiquitin Ligase E6AP at 2.55 A Resolution, PDB code: 4giz was solved by A.G.Mcewen, K.Zanier, S.Charbonnier, P.Poussin, V.Cura, S.Vande Pol, G.Trave, J.Cavarelli, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	37.33 / 2.55
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	106.279, 134.936, 138.794, 90.00, 90.00, 90.00
R / Rfree (%)	16.6 / 19.6

The binding sites of Zinc atom in the Crystal Structure of Full-Length Human Papillomavirus Oncoprotein E6 in Complex with Lxxll Peptide of Ubiquitin Ligase E6AP at 2.55 A Resolution (pdb code 4giz). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the Crystal Structure of Full-Length Human Papillomavirus Oncoprotein E6 in Complex with Lxxll Peptide of Ubiquitin Ligase E6AP at 2.55 A Resolution, PDB code: 4giz:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in the Crystal Structure of Full-Length Human Papillomavirus Oncoprotein E6 in Complex with Lxxll Peptide of Ubiquitin Ligase E6AP at 2.55 A Resolution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Full-Length Human Papillomavirus Oncoprotein E6 in Complex with Lxxll Peptide of Ubiquitin Ligase E6AP at 2.55 A Resolution within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn201 b:40.7 occ:1.00 SG C:CYS136 2.3 42.9 1.0 SG C:CYS106 2.3 40.0 1.0 SG C:CYS103 2.4 37.9 1.0 SG C:CYS139 2.4 44.4 1.0 CB C:CYS103 3.2 34.2 1.0 CB C:CYS139 3.2 42.2 1.0 CB C:CYS106 3.3 35.6 1.0 CB C:CYS136 3.5 40.7 1.0 N C:CYS106 3.7 37.0 1.0 O C:HOH363 4.0 51.7 1.0 CA C:CYS106 4.0 35.6 1.0 N C:CYS136 4.1 41.3 1.0 N C:CYS139 4.2 45.2 1.0 CA C:CYS139 4.3 44.5 1.0 CA C:CYS136 4.4 41.8 1.0 O C:HOH328 4.5 38.4 1.0 CA C:CYS103 4.6 33.9 1.0 CB C:ASN105 4.6 39.2 1.0 C C:CYS106 4.7 38.3 1.0 C C:ASN105 4.8 40.4 1.0 O C:HOH362 4.8 48.1 1.0 N C:GLN107 4.9 32.2 1.0 C C:CYS136 5.0 48.7 1.0 CB C:LYS108 5.0 38.7 1.0

Zinc binding site 2 out of 4 in the Crystal Structure of Full-Length Human Papillomavirus Oncoprotein E6 in Complex with Lxxll Peptide of Ubiquitin Ligase E6AP at 2.55 A Resolution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Full-Length Human Papillomavirus Oncoprotein E6 in Complex with Lxxll Peptide of Ubiquitin Ligase E6AP at 2.55 A Resolution within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn202 b:36.1 occ:1.00 SG C:CYS33 2.3 35.0 1.0 SG C:CYS66 2.3 38.6 1.0 SG C:CYS30 2.3 34.9 1.0 SG C:CYS63 2.4 36.1 1.0 CB C:CYS66 3.1 33.9 1.0 CB C:CYS30 3.1 31.1 1.0 CB C:CYS33 3.4 31.5 1.0 CB C:CYS63 3.5 31.7 1.0 N C:CYS33 3.7 31.9 1.0 N C:CYS63 4.1 31.8 1.0 CA C:CYS33 4.1 31.5 1.0 NZ D:LYS65 4.3 48.2 1.0 CA C:CYS63 4.3 31.2 1.0 CA C:CYS66 4.3 33.6 1.0 N C:CYS66 4.3 35.0 1.0 CE D:LYS65 4.5 54.2 1.0 CB C:TYR32 4.6 30.0 1.0 CA C:CYS30 4.6 31.5 1.0 O D:HOH324 4.8 41.0 1.0 C C:TYR32 4.8 36.4 1.0 C C:CYS63 4.9 34.8 1.0 O C:CYS63 4.9 35.8 1.0 C C:CYS33 4.9 35.5 1.0

Zinc binding site 3 out of 4 in the Crystal Structure of Full-Length Human Papillomavirus Oncoprotein E6 in Complex with Lxxll Peptide of Ubiquitin Ligase E6AP at 2.55 A Resolution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Crystal Structure of Full-Length Human Papillomavirus Oncoprotein E6 in Complex with Lxxll Peptide of Ubiquitin Ligase E6AP at 2.55 A Resolution within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn201 b:46.7 occ:1.00 SG D:CYS136 2.3 43.9 1.0 SG D:CYS106 2.3 44.6 1.0 SG D:CYS103 2.4 42.8 1.0 SG D:CYS139 2.4 55.5 1.0 CB D:CYS103 3.2 38.8 1.0 CB D:CYS139 3.2 53.2 1.0 CB D:CYS106 3.3 39.8 1.0 CB D:CYS136 3.6 41.5 1.0 N D:CYS106 3.7 41.9 1.0 CA D:CYS106 4.0 39.9 1.0 N D:CYS136 4.1 42.5 1.0 N D:CYS139 4.2 56.4 1.0 CA D:CYS139 4.3 54.9 1.0 CA D:CYS136 4.4 43.2 1.0 CB D:ASN105 4.5 39.9 0.5 CB D:ASN105 4.6 42.1 0.5 CA D:CYS103 4.6 39.1 1.0 C D:CYS106 4.7 42.8 1.0 C D:ASN105 4.7 46.2 0.5 C D:ASN105 4.8 46.6 0.5 N D:GLN107 4.9 38.4 1.0 C D:CYS136 5.0 52.6 1.0 CA D:ASN105 5.0 42.1 0.5

Zinc binding site 4 out of 4 in the Crystal Structure of Full-Length Human Papillomavirus Oncoprotein E6 in Complex with Lxxll Peptide of Ubiquitin Ligase E6AP at 2.55 A Resolution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Crystal Structure of Full-Length Human Papillomavirus Oncoprotein E6 in Complex with Lxxll Peptide of Ubiquitin Ligase E6AP at 2.55 A Resolution within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn202 b:38.6 occ:1.00 SG D:CYS33 2.3 35.4 1.0 SG D:CYS30 2.3 34.4 1.0 SG D:CYS63 2.4 36.4 1.0 SG D:CYS66 2.4 38.2 1.0 CB D:CYS30 3.1 30.9 1.0 CB D:CYS66 3.1 33.6 1.0 CB D:CYS33 3.4 32.5 1.0 CB D:CYS63 3.6 32.2 1.0 N D:CYS33 3.8 33.6 1.0 O D:HOH324 4.1 41.0 1.0 N D:CYS63 4.1 34.1 1.0 CA D:CYS33 4.2 32.8 1.0 N D:CYS66 4.3 34.8 1.0 CA D:CYS66 4.3 34.1 1.0 CA D:CYS63 4.4 32.6 1.0 CB D:TYR32 4.5 31.1 1.0 CA D:CYS30 4.6 31.4 1.0 O D:HOH364 4.6 58.3 1.0 O D:CYS63 4.8 38.1 1.0 C D:TYR32 4.8 39.1 1.0 C D:CYS63 4.9 38.7 1.0 C D:CYS33 5.0 37.0 1.0 N D:TYR32 5.0 31.7 1.0

K.Zanier, S.Charbonnier, A.O.Sidi, A.G.Mcewen, M.G.Ferrario, P.Poussin-Courmontagne, V.Cura, N.Brimer, K.O.Babah, T.Ansari, I.Muller, R.H.Stote, J.Cavarelli, S.Vande Pol, G.Trave. Structural Basis For Hijacking of Cellular Lxxll Motifs By Papillomavirus E6 Oncoproteins. Science V. 339 694 2013.
ISSN: ISSN 0036-8075
PubMed: 23393263
DOI: 10.1126/SCIENCE.1229934