Zinc in PDB 4fmp: Crystal Structure of Thermostable, Organic-Solvent Tolerant Lipase From Geobacillus Sp. Strain Arm

All present enzymatic activity of Crystal Structure of Thermostable, Organic-Solvent Tolerant Lipase From Geobacillus Sp. Strain Arm:
3.1.1.3;

The structure of Crystal Structure of Thermostable, Organic-Solvent Tolerant Lipase From Geobacillus Sp. Strain Arm, PDB code: 4fmp was solved by N.D.Nisbar, R.N.Z.R.A.Rahman, M.S.M.Ali, A.T.C.Leow, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 2.30
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	55.786, 143.392, 63.965, 90.00, 105.88, 90.00
R / Rfree (%)	17.3 / 24.2

The structure of Crystal Structure of Thermostable, Organic-Solvent Tolerant Lipase From Geobacillus Sp. Strain Arm also contains other interesting chemical elements:

Calcium

(Ca)

2 atoms

The binding sites of Zinc atom in the Crystal Structure of Thermostable, Organic-Solvent Tolerant Lipase From Geobacillus Sp. Strain Arm (pdb code 4fmp). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of Thermostable, Organic-Solvent Tolerant Lipase From Geobacillus Sp. Strain Arm, PDB code: 4fmp:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of Thermostable, Organic-Solvent Tolerant Lipase From Geobacillus Sp. Strain Arm


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Thermostable, Organic-Solvent Tolerant Lipase From Geobacillus Sp. Strain Arm within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn400 b:28.6 occ:1.00 OD2 A:ASP238 2.0 25.6 1.0 OD1 A:ASP61 2.0 27.1 1.0 NE2 A:HIS87 2.1 27.3 1.0 NE2 A:HIS81 2.1 18.4 1.0 CG A:ASP61 2.7 27.0 1.0 CG A:ASP238 2.8 29.8 1.0 OD2 A:ASP61 2.8 23.5 1.0 OD1 A:ASP238 3.0 28.2 1.0 CD2 A:HIS87 3.0 27.7 1.0 CE1 A:HIS81 3.0 19.1 1.0 CE1 A:HIS87 3.1 29.2 1.0 CD2 A:HIS81 3.1 18.2 1.0 OG A:SER58 4.0 18.1 1.0 ND1 A:HIS87 4.2 29.1 1.0 ND1 A:HIS81 4.2 26.0 1.0 CB A:ASP238 4.2 28.4 1.0 CG A:HIS87 4.2 27.8 1.0 CB A:ASP61 4.2 23.5 1.0 CG A:HIS81 4.2 27.5 1.0 O A:HOH517 4.2 22.8 1.0 CD1 A:TRP60 4.6 22.2 1.0 CA A:ASP61 4.8 23.4 1.0 CD2 A:HIS85 4.8 30.5 1.0 O A:ASP238 4.9 28.3 1.0 N A:ASP61 4.9 24.1 1.0 CZ2 A:TRP211 4.9 23.6 1.0 CB A:HIS85 4.9 29.1 1.0 CG A:HIS85 5.0 27.3 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of Thermostable, Organic-Solvent Tolerant Lipase From Geobacillus Sp. Strain Arm


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Thermostable, Organic-Solvent Tolerant Lipase From Geobacillus Sp. Strain Arm within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn400 b:34.0 occ:1.00 NE2 B:HIS87 2.0 27.1 1.0 OD2 B:ASP238 2.0 32.9 1.0 NE2 B:HIS81 2.0 27.2 1.0 OD1 B:ASP61 2.1 27.8 1.0 OD2 B:ASP61 2.7 24.0 1.0 CG B:ASP238 2.7 30.0 1.0 CG B:ASP61 2.8 27.9 1.0 OD1 B:ASP238 2.9 29.2 1.0 CE1 B:HIS87 2.9 27.2 1.0 CD2 B:HIS87 3.0 24.4 1.0 CD2 B:HIS81 3.0 27.7 1.0 CE1 B:HIS81 3.1 27.8 1.0 OG B:SER58 4.0 23.7 1.0 ND1 B:HIS87 4.0 27.9 1.0 CG B:HIS87 4.1 29.3 1.0 CG B:HIS81 4.2 28.8 1.0 ND1 B:HIS81 4.2 25.3 1.0 CB B:ASP238 4.2 29.3 1.0 CB B:ASP61 4.3 27.6 1.0 O B:HOH529 4.3 39.1 1.0 CD2 B:HIS85 4.7 30.1 1.0 CD1 B:TRP60 4.7 24.1 1.0 CZ2 B:TRP211 4.7 29.5 1.0 CB B:HIS85 4.9 30.8 1.0 CA B:ASP61 4.9 27.6 1.0 N B:ASP61 4.9 26.8 1.0 O B:ASP238 4.9 28.9 1.0 CG B:HIS85 4.9 31.2 1.0

N.D.Nisbar, R.N.Z.R.A.Rahman, M.S.M.Ali, A.T.C.Leow. Crystallization of Novel Arm Lipase and Elucidation of Its Space-Grown Crystal Structure Thesis 2013.