Zinc in PDB 4f51: Human Insulin

The structure of Human Insulin, PDB code: 4f51 was solved by M.P.Favero-Retto, L.C.Palmieri, L.M.T.R.Lima, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	23.67 / 1.64
Space group	H 3
Cell size a, b, c (Å), α, β, γ (°)	81.990, 81.990, 33.750, 90.00, 90.00, 120.00
R / Rfree (%)	15.2 / 21.2

The structure of Human Insulin also contains other interesting chemical elements:

Chlorine

(Cl)

2 atoms

The binding sites of Zinc atom in the Human Insulin (pdb code 4f51). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Human Insulin, PDB code: 4f51:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Human Insulin


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Human Insulin within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn101 b:11.3 occ:0.33 NE2 B:HIS10 2.0 10.5 1.0 CE1 B:HIS10 2.9 10.0 1.0 CL B:CL102 3.0 30.2 0.3 CD2 B:HIS10 3.1 7.5 1.0 ND1 B:HIS10 4.1 9.9 1.0 CG B:HIS10 4.1 8.4 1.0 O D:HOH232 4.4 16.6 0.3 O B:HOH206 4.9 18.9 1.0

Zinc binding site 2 out of 2 in the Human Insulin


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Human Insulin within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn101 b:9.4 occ:0.33 NE2 D:HIS10 2.0 7.7 1.0 CL D:CL102 2.2 40.1 0.3 CE1 D:HIS10 3.0 9.6 1.0 CD2 D:HIS10 3.1 7.8 1.0 O D:HOH219 3.8 34.7 1.0 O D:HOH230 3.9 22.6 1.0 ND1 D:HIS10 4.1 10.3 1.0 CG D:HIS10 4.2 8.8 1.0 O D:HOH203 4.5 20.0 1.0

M.P.Favero-Retto, L.C.Palmieri, T.A.Souza, F.C.Almeida, L.M.Lima. Structural Meta-Analysis of Regular Human Insulin in Pharmaceutical Formulations. Eur J Pharm Biopharm V. 85 1112 2013.
PubMed: 23692694
DOI: 10.1016/J.EJPB.2013.05.005