Zinc in PDB 4au7: The Structure of the SUV4-20H2 Ternary Complex with Histone H4

All present enzymatic activity of The Structure of the SUV4-20H2 Ternary Complex with Histone H4:
2.1.1.43;

The structure of The Structure of the SUV4-20H2 Ternary Complex with Histone H4, PDB code: 4au7 was solved by S.M.Southall, N.B.Cronin, J.R.Wilson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	55.33 / 2.07
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	37.270, 65.170, 209.490, 90.00, 90.00, 90.00
R / Rfree (%)	19.255 / 24.039

The binding sites of Zinc atom in the The Structure of the SUV4-20H2 Ternary Complex with Histone H4 (pdb code 4au7). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 3 binding sites of Zinc where determined in the The Structure of the SUV4-20H2 Ternary Complex with Histone H4, PDB code: 4au7:
Jump to Zinc binding site number: 1; 2; 3;

Zinc binding site 1 out of 3 in the The Structure of the SUV4-20H2 Ternary Complex with Histone H4


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of The Structure of the SUV4-20H2 Ternary Complex with Histone H4 within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1248 b:27.1 occ:1.00 SG A:CYS231 2.2 25.0 1.0 SG A:CYS185 2.3 28.2 1.0 SG A:CYS229 2.3 24.1 1.0 SG A:CYS234 2.4 28.4 1.0 CB A:CYS231 3.3 21.7 1.0 CB A:CYS229 3.3 25.3 1.0 CB A:CYS234 3.4 27.1 1.0 CB A:CYS185 3.4 28.7 1.0 N A:CYS185 3.9 26.4 1.0 N A:CYS234 4.0 27.3 1.0 N A:CYS231 4.0 23.6 1.0 NE2 A:HIS183 4.1 26.6 1.0 CA A:CYS231 4.2 24.3 1.0 CA A:CYS185 4.2 26.9 1.0 CD2 A:HIS183 4.2 26.0 1.0 CA A:CYS234 4.2 28.4 1.0 O A:HOH2093 4.4 33.4 1.0 O A:HOH2089 4.5 41.8 1.0 CA A:CYS229 4.6 26.1 1.0 C A:CYS229 4.6 26.2 1.0 C A:CYS231 4.8 25.6 1.0 O A:CYS231 4.8 26.3 1.0 C A:ASP184 4.9 25.9 1.0 N A:GLU230 4.9 23.6 1.0 O A:CYS229 4.9 24.2 1.0

Zinc binding site 2 out of 3 in the The Structure of the SUV4-20H2 Ternary Complex with Histone H4


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of The Structure of the SUV4-20H2 Ternary Complex with Histone H4 within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1249 b:27.7 occ:0.30 SG A:CYS141 2.0 40.5 1.0 O A:HOH2033 2.1 35.3 1.0 SG A:CYS95 2.5 46.2 1.0 ND1 A:HIS92 2.6 45.1 1.0 CB A:CYS141 3.1 36.7 1.0 CE1 A:HIS92 3.3 47.3 1.0 CB A:CYS95 3.4 36.7 1.0 CG A:HIS92 3.6 36.7 1.0 CA A:HIS92 3.6 28.9 1.0 CB A:HIS92 4.1 31.5 1.0 O A:HIS92 4.2 28.7 1.0 NE2 A:HIS92 4.4 39.5 1.0 CA A:CYS141 4.4 32.5 1.0 O A:THR91 4.4 32.7 1.0 C A:HIS92 4.4 27.9 1.0 CD2 A:HIS92 4.5 38.6 1.0 N A:HIS92 4.5 31.4 1.0 O A:HOH2034 4.7 42.7 1.0 C A:THR91 4.7 34.3 1.0 CA A:CYS95 4.8 35.2 1.0 N A:CYS141 4.9 31.8 1.0 CG2 A:VAL139 5.0 31.4 1.0

Zinc binding site 3 out of 3 in the The Structure of the SUV4-20H2 Ternary Complex with Histone H4


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of The Structure of the SUV4-20H2 Ternary Complex with Histone H4 within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn1219 b:37.4 occ:0.30 SG B:CYS185 3.0 63.5 1.0 CB B:CYS185 3.8 54.1 1.0 N B:CYS185 3.9 50.2 1.0 CA B:CYS185 4.5 50.3 1.0 CD2 B:HIS183 4.7 66.5 1.0 CA B:ASP184 4.7 50.2 1.0 C B:ASP184 4.8 51.9 1.0 NE2 B:HIS183 4.9 68.3 1.0

S.M.Southall, N.B.Cronin, J.R.Wilson. A Novel Route to Product Specificity in the SUV4-20 Family of Histone H4K20 Methyltransferases. Nucleic Acids Res. V. 42 661 2014.
ISSN: ISSN 0305-1048
PubMed: 24049080
DOI: 10.1093/NAR/GKT776