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Zinc in PDB 4a3e: Rna Polymerase II Initial Transcribing Complex with A 5NT Dna-Rna Hybrid and Soaked with Ampcpp

Enzymatic activity of Rna Polymerase II Initial Transcribing Complex with A 5NT Dna-Rna Hybrid and Soaked with Ampcpp

All present enzymatic activity of Rna Polymerase II Initial Transcribing Complex with A 5NT Dna-Rna Hybrid and Soaked with Ampcpp:
2.7.7.6;

Protein crystallography data

The structure of Rna Polymerase II Initial Transcribing Complex with A 5NT Dna-Rna Hybrid and Soaked with Ampcpp, PDB code: 4a3e was solved by A.C.M.Cheung, S.Sainsbury, P.Cramer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 54.61 / 3.40
Space group C 2 2 21
Cell size a, b, c (Å), α, β, γ (°) 223.680, 394.170, 283.290, 90.00, 90.00, 90.00
R / Rfree (%) 16 / 18.8

Other elements in 4a3e:

The structure of Rna Polymerase II Initial Transcribing Complex with A 5NT Dna-Rna Hybrid and Soaked with Ampcpp also contains other interesting chemical elements:

Magnesium (Mg) 1 atom
Bromine (Br) 1 atom

Zinc Binding Sites:

The binding sites of Zinc atom in the Rna Polymerase II Initial Transcribing Complex with A 5NT Dna-Rna Hybrid and Soaked with Ampcpp (pdb code 4a3e). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 8 binding sites of Zinc where determined in the Rna Polymerase II Initial Transcribing Complex with A 5NT Dna-Rna Hybrid and Soaked with Ampcpp, PDB code: 4a3e:
Jump to Zinc binding site number: 1; 2; 3; 4; 5; 6; 7; 8;

Zinc binding site 1 out of 8 in 4a3e

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Zinc binding site 1 out of 8 in the Rna Polymerase II Initial Transcribing Complex with A 5NT Dna-Rna Hybrid and Soaked with Ampcpp


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Rna Polymerase II Initial Transcribing Complex with A 5NT Dna-Rna Hybrid and Soaked with Ampcpp within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn2456

b:0.5
occ:1.00
 SG A:CYS148 2.3 0.1 1.0
SG A:CYS107 2.3 0.5 1.0
SG A:CYS110 2.4 0.3 1.0
SG A:CYS167 2.4 0.2 1.0
CB A:CYS167 2.7 0.8 1.0
CB A:CYS148 3.1 0.5 1.0
CB A:CYS110 3.2 0.0 1.0
CB A:CYS107 3.3 1.0 1.0
N A:CYS110 3.7 0.7 1.0
CA A:CYS110 4.0 0.6 1.0
O A:GLY166 4.0 0.4 1.0
CA A:CYS167 4.0 0.6 1.0
C A:CYS167 4.3 0.8 1.0
O A:CYS167 4.3 0.3 1.0
CA A:CYS148 4.5 0.7 1.0
CA A:CYS107 4.7 1.0 1.0
C A:GLY166 4.8 0.6 1.0
N A:CYS167 4.8 1.0 1.0
NE2 A:GLN171 4.8 0.7 1.0
C A:CYS110 4.9 0.5 1.0
C A:HIS109 4.9 0.2 1.0
N A:GLY111 4.9 0.1 1.0
CB A:HIS109 5.0 0.4 1.0
CD2 A:HIS109 5.0 0.3 1.0

Zinc binding site 2 out of 8 in 4a3e

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Zinc binding site 2 out of 8 in the Rna Polymerase II Initial Transcribing Complex with A 5NT Dna-Rna Hybrid and Soaked with Ampcpp


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Rna Polymerase II Initial Transcribing Complex with A 5NT Dna-Rna Hybrid and Soaked with Ampcpp within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn2457

b:85.4
occ:1.00
 NE2 A:HIS80 2.2 98.2 1.0
SG A:CYS77 2.4 90.0 1.0
SG A:CYS67 2.5 98.7 1.0
SG A:CYS70 2.5 93.5 1.0
CD2 A:HIS80 2.6 98.8 1.0
O A:THR69 3.2 97.2 1.0
CB A:CYS70 3.3 91.2 1.0
CE1 A:HIS80 3.4 98.1 1.0
CB A:CYS67 3.5 95.0 1.0
CB A:CYS77 3.7 85.6 1.0
CG A:HIS80 3.9 97.5 1.0
O A:CYS70 4.1 98.9 1.0
O A:PRO78 4.2 93.5 1.0
C A:THR69 4.2 97.3 1.0
ND1 A:HIS80 4.3 98.7 1.0
CA A:CYS70 4.4 92.0 1.0
CA A:CYS77 4.5 85.7 1.0
C A:CYS70 4.6 97.7 1.0
N A:CYS70 4.7 93.1 1.0
CA A:CYS67 4.9 96.0 1.0

Zinc binding site 3 out of 8 in 4a3e

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Zinc binding site 3 out of 8 in the Rna Polymerase II Initial Transcribing Complex with A 5NT Dna-Rna Hybrid and Soaked with Ampcpp


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Rna Polymerase II Initial Transcribing Complex with A 5NT Dna-Rna Hybrid and Soaked with Ampcpp within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn2225

b:95.6
occ:1.00
 SG B:CYS1182 2.3 96.3 1.0
SG B:CYS1166 2.3 99.1 1.0
SG B:CYS1185 2.3 0.1 1.0
SG B:CYS1163 2.3 94.8 1.0
CB B:CYS1163 3.0 89.3 1.0
CB B:CYS1185 3.1 97.8 1.0
CB B:CYS1166 3.7 95.1 1.0
CB B:CYS1182 3.7 93.4 1.0
O B:CYS1166 3.8 94.8 1.0
N B:CYS1166 3.9 94.2 1.0
CA B:CYS1166 4.3 93.7 1.0
CA B:CYS1185 4.4 99.4 1.0
C B:CYS1166 4.5 95.0 1.0
CA B:CYS1163 4.5 87.5 1.0
OG1 B:THR1170 4.6 1.0 1.0
O B:CYS1182 4.6 0.9 1.0
CB B:THR1170 4.6 0.2 1.0
N B:CYS1185 4.6 0.4 1.0
CG2 B:THR1170 4.7 0.8 1.0
CB B:ILE1165 4.9 90.9 1.0
CA B:CYS1182 5.0 94.3 1.0

Zinc binding site 4 out of 8 in 4a3e

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Zinc binding site 4 out of 8 in the Rna Polymerase II Initial Transcribing Complex with A 5NT Dna-Rna Hybrid and Soaked with Ampcpp


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Rna Polymerase II Initial Transcribing Complex with A 5NT Dna-Rna Hybrid and Soaked with Ampcpp within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Zn1269

b:96.5
occ:1.00
 SG C:CYS92 2.2 89.8 1.0
SG C:CYS95 2.2 90.0 1.0
SG C:CYS86 2.3 85.0 1.0
SG C:CYS88 2.3 87.5 1.0
CB C:CYS86 3.3 80.1 1.0
CB C:CYS95 3.4 87.1 1.0
CB C:CYS92 3.4 87.0 1.0
N C:CYS92 3.5 88.2 1.0
CB C:CYS88 3.7 83.7 1.0
C C:HIS91 3.9 93.1 1.0
CA C:CYS92 3.9 87.7 1.0
N C:CYS95 4.1 90.1 1.0
CA C:HIS91 4.1 88.7 1.0
CA C:CYS95 4.3 88.5 1.0
N C:CYS88 4.4 79.5 1.0
C C:CYS92 4.4 93.6 1.0
CB C:LYS94 4.6 90.4 1.0
O C:CYS92 4.6 92.0 1.0
O C:HIS91 4.6 93.9 1.0
CA C:CYS86 4.6 80.0 1.0
O C:ASP90 4.7 95.9 1.0
CA C:CYS88 4.7 82.6 1.0
C C:LYS94 4.9 94.8 1.0
C C:CYS86 4.9 81.9 1.0
N C:LYS94 4.9 90.3 1.0

Zinc binding site 5 out of 8 in 4a3e

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Zinc binding site 5 out of 8 in the Rna Polymerase II Initial Transcribing Complex with A 5NT Dna-Rna Hybrid and Soaked with Ampcpp


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 5 of Rna Polymerase II Initial Transcribing Complex with A 5NT Dna-Rna Hybrid and Soaked with Ampcpp within 5.0Å range:
probe atom residue distance (Å) B Occ
I:Zn1121

b:0.7
occ:1.00
 SG I:CYS7 2.2 0.8 1.0
SG I:CYS32 2.3 1.0 1.0
SG I:CYS29 2.3 0.9 1.0
SG I:CYS10 2.3 0.3 1.0
CB I:CYS10 2.6 0.1 1.0
CB I:CYS29 3.3 0.7 1.0
CB I:CYS32 3.5 0.9 1.0
CB I:CYS7 3.5 0.4 1.0
CA I:CYS10 3.8 0.3 1.0
N I:CYS10 3.8 0.4 1.0
N I:CYS32 3.8 0.8 1.0
CA I:CYS32 4.2 0.1 1.0
OG1 I:THR31 4.2 0.3 1.0
CB I:TYR34 4.4 0.4 1.0
O I:ARG8 4.4 0.8 1.0
CB I:THR31 4.6 0.6 1.0
CA I:CYS29 4.7 0.3 1.0
C I:THR31 4.7 0.6 1.0
C I:CYS32 4.7 0.9 1.0
CA I:CYS7 4.8 0.3 1.0
C I:ASP9 4.8 0.6 1.0
O I:CYS7 4.9 0.9 1.0
C I:CYS10 4.9 0.8 1.0
C I:CYS7 4.9 0.7 1.0
O I:CYS32 4.9 1.0 1.0
CB I:ASP9 5.0 0.8 1.0
N I:ASN11 5.0 0.5 1.0

Zinc binding site 6 out of 8 in 4a3e

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Zinc binding site 6 out of 8 in the Rna Polymerase II Initial Transcribing Complex with A 5NT Dna-Rna Hybrid and Soaked with Ampcpp


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 6 of Rna Polymerase II Initial Transcribing Complex with A 5NT Dna-Rna Hybrid and Soaked with Ampcpp within 5.0Å range:
probe atom residue distance (Å) B Occ
I:Zn1122

b:1.0
occ:1.00
 SG I:CYS106 2.3 0.1 1.0
SG I:CYS78 2.4 0.7 1.0
SG I:CYS75 2.4 0.7 1.0
SG I:CYS103 2.4 0.8 1.0
CB I:CYS75 3.1 0.3 1.0
CB I:CYS103 3.1 0.6 1.0
CB I:CYS78 3.5 0.9 1.0
CB I:CYS106 3.5 0.3 1.0
N I:CYS78 3.8 0.4 1.0
OG I:SER105 3.9 0.0 1.0
CA I:CYS78 4.1 0.8 1.0
N I:CYS106 4.2 0.9 1.0
CA I:CYS106 4.4 0.8 1.0
CB I:HIS108 4.5 0.2 1.0
CA I:CYS75 4.5 0.6 1.0
CA I:CYS103 4.6 0.6 1.0
CB I:SER80 4.6 0.9 1.0
C I:CYS78 4.7 0.2 1.0
N I:SER107 4.8 0.2 1.0
CB I:LYS77 4.8 0.2 1.0
N I:HIS79 4.8 0.7 1.0
N I:HIS108 4.8 0.1 1.0
N I:SER80 4.9 0.7 1.0
C I:LYS77 4.9 0.7 1.0
C I:CYS106 4.9 0.5 1.0

Zinc binding site 7 out of 8 in 4a3e

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Zinc binding site 7 out of 8 in the Rna Polymerase II Initial Transcribing Complex with A 5NT Dna-Rna Hybrid and Soaked with Ampcpp


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 7 of Rna Polymerase II Initial Transcribing Complex with A 5NT Dna-Rna Hybrid and Soaked with Ampcpp within 5.0Å range:
probe atom residue distance (Å) B Occ
J:Zn1066

b:98.4
occ:1.00
 SG J:CYS10 2.2 99.5 1.0
SG J:CYS7 2.3 0.1 1.0
SG J:CYS45 2.3 97.3 1.0
SG J:CYS46 2.4 99.5 1.0
CB J:CYS7 3.1 99.3 1.0
N J:CYS46 3.2 94.7 1.0
CB J:CYS10 3.3 95.0 1.0
CB J:CYS45 3.4 92.9 1.0
CA J:CYS46 3.5 94.3 1.0
CB J:CYS46 3.5 95.4 1.0
C J:CYS45 3.6 98.2 1.0
N J:CYS10 3.8 92.0 1.0
CA J:CYS10 4.1 93.4 1.0
CA J:CYS45 4.1 93.3 1.0
NH2 J:ARG43 4.2 0.1 1.0
O J:CYS45 4.3 98.6 1.0
NE J:ARG43 4.4 0.9 1.0
CA J:CYS7 4.5 98.1 1.0
N J:GLY11 4.7 95.2 1.0
CZ J:ARG43 4.8 0.3 1.0
CB J:SER9 4.8 92.0 1.0
C J:CYS10 4.8 97.8 1.0
C J:SER9 4.8 93.3 1.0
N J:CYS45 4.8 94.7 1.0
CB J:LYS12 4.8 0.2 1.0
CB J:ARG43 4.9 0.2 1.0
C J:CYS7 4.9 97.5 1.0
N J:LYS12 4.9 96.1 1.0

Zinc binding site 8 out of 8 in 4a3e

Go back to Zinc Binding Sites List in 4a3e
Zinc binding site 8 out of 8 in the Rna Polymerase II Initial Transcribing Complex with A 5NT Dna-Rna Hybrid and Soaked with Ampcpp


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 8 of Rna Polymerase II Initial Transcribing Complex with A 5NT Dna-Rna Hybrid and Soaked with Ampcpp within 5.0Å range:
probe atom residue distance (Å) B Occ
L:Zn1071

b:0.3
occ:1.00
 SG L:CYS51 2.3 0.5 1.0
SG L:CYS48 2.3 0.9 1.0
SG L:CYS34 2.4 0.7 1.0
SG L:CYS31 2.4 0.0 1.0
CB L:CYS48 3.2 0.7 1.0
CB L:CYS31 3.5 0.8 1.0
CB L:CYS34 3.7 0.6 1.0
CB L:CYS51 3.9 0.7 1.0
N L:CYS51 4.3 0.9 1.0
N L:CYS34 4.4 0.6 1.0
OG L:SER36 4.5 0.7 1.0
CA L:CYS51 4.6 0.4 1.0
OE2 L:GLU33 4.6 0.1 1.0
CA L:CYS34 4.6 0.0 1.0
CA L:CYS48 4.6 0.4 1.0
CG L:GLU33 4.7 0.1 1.0
C L:CYS51 4.8 0.6 1.0
CA L:CYS31 5.0 0.2 1.0
O L:CYS51 5.0 0.2 1.0

Reference:

A.C.Cheung, S.Sainsbury, P.Cramer. Structural Basis of Initial Rna Polymerase II Transcription. Embo J. V. 30 4755 2011.
ISSN: ESSN 1460-2075
PubMed: 22056778
DOI: 10.1038/EMBOJ.2011.396
Page generated: Sat Oct 26 18:52:08 2024

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