Zinc in PDB 3ts0: Mouse LIN28A in Complex with Let-7F-1 Microrna Pre-Element

The structure of Mouse LIN28A in Complex with Let-7F-1 Microrna Pre-Element, PDB code: 3ts0 was solved by Y.Nam, P.Sliz, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	72.98 / 2.76
Space group	P 41 21 2
Cell size a, b, c (Å), α, β, γ (°)	139.880, 139.880, 85.550, 90.00, 90.00, 90.00
R / Rfree (%)	19.3 / 21.4

The binding sites of Zinc atom in the Mouse LIN28A in Complex with Let-7F-1 Microrna Pre-Element (pdb code 3ts0). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the Mouse LIN28A in Complex with Let-7F-1 Microrna Pre-Element, PDB code: 3ts0:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in the Mouse LIN28A in Complex with Let-7F-1 Microrna Pre-Element


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Mouse LIN28A in Complex with Let-7F-1 Microrna Pre-Element within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn200 b:88.7 occ:1.00 SG A:CYS152 2.2 78.5 1.0 SG A:CYS142 2.2 80.4 1.0 SG A:CYS139 2.4 78.7 1.0 NE2 A:HIS147 2.5 85.2 1.0 CB A:CYS152 3.0 78.7 1.0 CB A:CYS139 3.2 75.2 1.0 CE1 A:HIS147 3.3 85.0 1.0 CB A:CYS142 3.4 73.3 1.0 CD2 A:HIS147 3.5 87.7 1.0 N A:CYS142 3.7 70.2 1.0 CA A:CYS152 3.9 83.5 1.0 CA A:CYS142 4.1 70.4 1.0 ND1 A:HIS147 4.4 87.6 1.0 CB A:ASN141 4.5 59.7 1.0 CG A:HIS147 4.5 87.0 1.0 C A:ASN141 4.7 72.2 1.0 CA A:CYS139 4.7 73.6 1.0 N A:GLY143 4.8 70.3 1.0 N A:CYS152 4.8 87.6 1.0 C A:CYS142 4.9 75.7 1.0 CA A:ASN141 4.9 65.5 1.0 N A:ASN141 5.0 67.5 1.0 N A:GLY144 5.0 73.8 1.0

Zinc binding site 2 out of 4 in the Mouse LIN28A in Complex with Let-7F-1 Microrna Pre-Element


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Mouse LIN28A in Complex with Let-7F-1 Microrna Pre-Element within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn201 b:57.1 occ:1.00 NE2 A:HIS169 1.9 55.3 1.0 SG A:CYS164 2.2 53.0 1.0 SG A:CYS174 2.3 60.8 1.0 SG A:CYS161 2.3 57.2 1.0 CD2 A:HIS169 2.9 55.2 1.0 CE1 A:HIS169 2.9 54.2 1.0 CB A:CYS174 3.1 56.5 1.0 CB A:CYS161 3.1 52.8 1.0 CB A:CYS164 3.5 50.3 1.0 N A:CYS164 3.8 52.3 1.0 CA A:CYS174 3.9 55.2 1.0 CG A:HIS169 4.0 52.9 1.0 ND1 A:HIS169 4.1 53.8 1.0 CA A:CYS164 4.2 51.5 1.0 CB A:SER166 4.6 52.6 1.0 CA A:CYS161 4.6 53.0 1.0 CB A:PHE163 4.6 51.4 1.0 CD A:PRO175 4.7 55.9 1.0 C A:CYS174 4.8 59.4 1.0 C A:CYS164 4.8 55.5 1.0 C A:PHE163 4.8 57.1 1.0 N A:SER166 4.9 50.8 1.0 N A:CYS174 5.0 55.1 1.0 N A:PHE163 5.0 50.2 1.0 N A:GLN165 5.0 51.5 1.0 N A:PRO175 5.0 55.1 1.0

Zinc binding site 3 out of 4 in the Mouse LIN28A in Complex with Let-7F-1 Microrna Pre-Element


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Mouse LIN28A in Complex with Let-7F-1 Microrna Pre-Element within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn200 b:90.4 occ:1.00 SG B:CYS152 2.2 86.7 1.0 SG B:CYS142 2.3 81.5 1.0 NE2 B:HIS147 2.4 90.7 1.0 SG B:CYS139 2.5 81.1 1.0 CE1 B:HIS147 3.1 89.9 1.0 CB B:CYS152 3.2 87.5 1.0 CB B:CYS142 3.3 75.3 1.0 CB B:CYS139 3.4 77.7 1.0 CD2 B:HIS147 3.5 93.9 1.0 N B:CYS142 3.8 75.1 1.0 CA B:CYS152 3.9 92.1 1.0 CA B:CYS142 4.2 73.7 1.0 CB B:ASN141 4.3 75.1 1.0 ND1 B:HIS147 4.3 92.9 1.0 CG B:HIS147 4.5 93.4 1.0 C B:ASN141 4.6 78.5 1.0 ND2 B:ASN141 4.8 90.2 1.0 N B:CYS152 4.8 96.8 1.0 CA B:CYS139 4.8 77.0 1.0 CA B:ASN141 4.8 75.1 1.0 N B:ASN141 5.0 76.3 1.0 C B:CYS142 5.0 80.8 1.0 N B:GLY143 5.0 77.0 1.0

Zinc binding site 4 out of 4 in the Mouse LIN28A in Complex with Let-7F-1 Microrna Pre-Element


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Mouse LIN28A in Complex with Let-7F-1 Microrna Pre-Element within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn201 b:59.4 occ:1.00 NE2 B:HIS169 1.9 64.8 1.0 SG B:CYS164 2.3 53.8 1.0 SG B:CYS174 2.3 64.9 1.0 SG B:CYS161 2.3 62.5 1.0 CD2 B:HIS169 2.9 65.8 1.0 CE1 B:HIS169 3.0 63.5 1.0 CB B:CYS174 3.1 61.3 1.0 CB B:CYS161 3.1 58.5 1.0 CB B:CYS164 3.4 50.6 1.0 CA B:CYS174 3.8 60.6 1.0 N B:CYS164 3.8 53.6 1.0 CG B:HIS169 4.1 63.7 1.0 ND1 B:HIS169 4.1 64.2 1.0 CA B:CYS164 4.2 51.7 1.0 CB B:SER166 4.6 54.0 1.0 CA B:CYS161 4.6 59.2 1.0 CB B:PHE163 4.6 56.6 1.0 C B:CYS174 4.6 64.9 1.0 C B:CYS164 4.7 55.3 1.0 CD B:PRO175 4.7 62.1 1.0 C B:PHE163 4.8 60.6 1.0 N B:SER166 4.8 53.6 1.0 N B:CYS174 4.9 61.7 1.0 N B:PHE163 4.9 57.8 1.0 N B:GLN165 4.9 52.3 1.0 N B:PRO175 5.0 60.8 1.0

Y.Nam, C.Chen, R.I.Gregory, J.J.Chou, P.Sliz. Molecular Basis For Interaction of Let-7 Micrornas with LIN28. Cell(Cambridge,Mass.) V. 147 1080 2011.
ISSN: ISSN 0092-8674
PubMed: 22078496
DOI: 10.1016/J.CELL.2011.10.020