Zinc in PDB 3sjd: Crystal Structure of S. Cerevisiae GET3 with Bound Adp-MG2+ in Complex with GET2 Cytosolic Domain

The structure of Crystal Structure of S. Cerevisiae GET3 with Bound Adp-MG2+ in Complex with GET2 Cytosolic Domain, PDB code: 3sjd was solved by S.Reitz, K.Wild, I.Sinning, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	56.23 / 4.60
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	149.640, 209.750, 133.240, 90.00, 90.00, 90.00
R / Rfree (%)	32.3 / 38.8

The structure of Crystal Structure of S. Cerevisiae GET3 with Bound Adp-MG2+ in Complex with GET2 Cytosolic Domain also contains other interesting chemical elements:

Magnesium

(Mg)

3 atoms

The binding sites of Zinc atom in the Crystal Structure of S. Cerevisiae GET3 with Bound Adp-MG2+ in Complex with GET2 Cytosolic Domain (pdb code 3sjd). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of S. Cerevisiae GET3 with Bound Adp-MG2+ in Complex with GET2 Cytosolic Domain, PDB code: 3sjd:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of S. Cerevisiae GET3 with Bound Adp-MG2+ in Complex with GET2 Cytosolic Domain


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of S. Cerevisiae GET3 with Bound Adp-MG2+ in Complex with GET2 Cytosolic Domain within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn363 b:0.2 occ:1.00 SG B:CYS288 2.0 0.1 1.0 SG A:CYS285 2.6 0.2 1.0 SG A:CYS288 2.7 0.2 1.0 CB B:CYS288 2.9 0.6 1.0 SG B:CYS285 3.3 0.9 1.0 CA B:CYS288 3.8 0.6 1.0 CB A:CYS285 4.2 0.9 1.0 CB A:CYS288 4.4 0.6 1.0 N B:CYS285 4.7 0.0 1.0 N B:CYS288 4.7 0.8 1.0 CB B:CYS285 4.8 0.5 1.0 C B:CYS288 4.9 0.1 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of S. Cerevisiae GET3 with Bound Adp-MG2+ in Complex with GET2 Cytosolic Domain


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of S. Cerevisiae GET3 with Bound Adp-MG2+ in Complex with GET2 Cytosolic Domain within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn363 b:0.8 occ:0.50 SG C:CYS288 1.9 0.1 1.0 SG C:CYS285 2.1 0.8 1.0 CB C:CYS288 3.6 0.3 1.0 CB C:CYS285 3.8 0.5 1.0 N C:CYS285 4.1 0.8 1.0 OD1 C:ASN284 4.4 0.2 1.0 CA C:CYS285 4.5 0.6 1.0 CA C:CYS288 4.5 0.7 1.0 N C:CYS288 4.9 0.8 1.0 O C:CYS285 4.9 0.9 1.0

S.Stefer, S.Reitz, F.Wang, K.Wild, Y.Y.Pang, D.Schwarz, J.Bomke, C.Hein, F.Lohr, F.Bernhard, V.Denic, V.Dotsch, I.Sinning. Structural Basis For Tail-Anchored Membrane Protein Biogenesis By the GET3-Receptor Complex. Science V. 333 758 2011.
ISSN: ISSN 0036-8075
PubMed: 21719644
DOI: 10.1126/SCIENCE.1207125