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Zinc in PDB 3sjc: Crystal Structure of S.Cerevisiae GET3 in the Semi-Open State in Complex with GET1 Cytosolic Domain

Protein crystallography data

The structure of Crystal Structure of S.Cerevisiae GET3 in the Semi-Open State in Complex with GET1 Cytosolic Domain, PDB code: 3sjc was solved by S.Reitz, K.Wild, I.Sinning, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 47.44 / 3.20
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 65.071, 112.243, 135.978, 90.00, 103.82, 90.00
R / Rfree (%) 20.9 / 25.9

Zinc Binding Sites:

The binding sites of Zinc atom in the Crystal Structure of S.Cerevisiae GET3 in the Semi-Open State in Complex with GET1 Cytosolic Domain (pdb code 3sjc). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of S.Cerevisiae GET3 in the Semi-Open State in Complex with GET1 Cytosolic Domain, PDB code: 3sjc:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in 3sjc

Go back to Zinc Binding Sites List in 3sjc
Zinc binding site 1 out of 2 in the Crystal Structure of S.Cerevisiae GET3 in the Semi-Open State in Complex with GET1 Cytosolic Domain


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of S.Cerevisiae GET3 in the Semi-Open State in Complex with GET1 Cytosolic Domain within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn363

b:0.2
occ:1.00
SG B:CYS288 2.3 0.7 1.0
SG A:CYS285 2.5 0.5 1.0
SG B:CYS285 2.6 0.0 1.0
SG A:CYS288 2.6 0.9 1.0
CB B:CYS288 3.6 0.5 1.0
N B:CYS288 3.9 0.9 1.0
CB A:CYS288 4.0 94.0 1.0
CA B:CYS288 4.2 90.0 1.0
N A:CYS288 4.2 76.0 1.0
CB B:CYS285 4.2 0.7 1.0
CB A:CYS285 4.2 0.4 1.0
CA A:CYS288 4.5 87.3 1.0
CB A:ARG287 4.6 67.9 1.0
CG A:ARG287 4.6 68.8 1.0
N A:CYS285 4.6 0.9 1.0
C B:ARG287 4.6 0.5 1.0
CB B:ARG287 4.7 0.5 1.0
C A:ARG287 4.9 77.5 1.0
CA A:CYS285 4.9 0.3 1.0
N B:ARG287 4.9 0.2 1.0

Zinc binding site 2 out of 2 in 3sjc

Go back to Zinc Binding Sites List in 3sjc
Zinc binding site 2 out of 2 in the Crystal Structure of S.Cerevisiae GET3 in the Semi-Open State in Complex with GET1 Cytosolic Domain


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of S.Cerevisiae GET3 in the Semi-Open State in Complex with GET1 Cytosolic Domain within 5.0Å range:
probe atom residue distance (Å) B Occ
E:Zn363

b:0.2
occ:1.00
SG E:CYS285 2.5 0.1 1.0
SG F:CYS288 2.5 0.1 1.0
SG E:CYS288 2.5 0.1 1.0
SG F:CYS285 3.0 0.4 1.0
N E:CYS288 3.6 0.1 1.0
CB E:CYS288 3.7 0.3 1.0
CB F:CYS288 4.0 0.6 1.0
CA E:CYS288 4.0 0.6 1.0
CB E:CYS285 4.1 0.7 1.0
CB E:ARG287 4.2 0.5 1.0
C E:ARG287 4.2 0.7 1.0
N F:CYS288 4.3 0.5 1.0
CB F:CYS285 4.5 0.3 1.0
N E:ARG287 4.5 0.9 1.0
CA E:ARG287 4.5 0.7 1.0
CA F:CYS288 4.6 0.3 1.0

Reference:

S.Stefer, S.Reitz, F.Wang, K.Wild, Y.Y.Pang, D.Schwarz, J.Bomke, C.Hein, F.Lohr, F.Bernhard, V.Denic, V.Dotsch, I.Sinning. Structural Basis For Tail-Anchored Membrane Protein Biogenesis By the GET3-Receptor Complex. Science V. 333 758 2011.
ISSN: ISSN 0036-8075
PubMed: 21719644
DOI: 10.1126/SCIENCE.1207125
Page generated: Sat Oct 26 15:51:42 2024

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