Zinc in PDB 3sja: Crystal Structure of S. Cerevisiae GET3 in the Open State in Complex with GET1 Cytosolic Domain

The structure of Crystal Structure of S. Cerevisiae GET3 in the Open State in Complex with GET1 Cytosolic Domain, PDB code: 3sja was solved by S.Reitz, K.Wild, I.Sinning, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	51.30 / 3.00
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	204.120, 91.460, 149.180, 90.00, 90.00, 90.00
R / Rfree (%)	18.1 / 23.1

The binding sites of Zinc atom in the Crystal Structure of S. Cerevisiae GET3 in the Open State in Complex with GET1 Cytosolic Domain (pdb code 3sja). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 3 binding sites of Zinc where determined in the Crystal Structure of S. Cerevisiae GET3 in the Open State in Complex with GET1 Cytosolic Domain, PDB code: 3sja:
Jump to Zinc binding site number: 1; 2; 3;

Zinc binding site 1 out of 3 in the Crystal Structure of S. Cerevisiae GET3 in the Open State in Complex with GET1 Cytosolic Domain


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of S. Cerevisiae GET3 in the Open State in Complex with GET1 Cytosolic Domain within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn363 b:79.5 occ:1.00 SG B:CYS285 1.9 87.3 1.0 SG A:CYS285 2.1 89.8 1.0 SG A:CYS288 2.2 73.2 1.0 SG B:CYS288 2.4 75.9 1.0 CB A:CYS285 3.1 45.7 1.0 CB A:CYS288 3.4 35.4 1.0 CB B:CYS285 3.5 0.6 1.0 CB B:CYS288 3.7 36.8 1.0 N A:CYS288 3.7 60.5 1.0 N B:CYS288 3.8 47.9 1.0 CA A:CYS288 4.0 67.5 1.0 CA B:CYS288 4.3 52.2 1.0 CA B:CYS285 4.5 0.7 1.0 CB B:ARG287 4.5 56.3 1.0 N B:CYS285 4.5 0.3 1.0 CA A:CYS285 4.5 80.7 1.0 CB A:ARG287 4.6 53.8 1.0 C B:ARG287 4.7 65.5 1.0 C A:ARG287 4.7 70.0 1.0 O B:CYS285 4.7 78.8 1.0 C B:CYS285 4.8 0.3 1.0 CA B:ARG287 4.9 64.8 1.0 O B:HIS283 5.0 0.8 1.0

Zinc binding site 2 out of 3 in the Crystal Structure of S. Cerevisiae GET3 in the Open State in Complex with GET1 Cytosolic Domain


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of S. Cerevisiae GET3 in the Open State in Complex with GET1 Cytosolic Domain within 5.0Å range: probe atom residue distance (Å) B Occ E:Zn363 b:82.5 occ:1.00 SG E:CYS285 2.1 90.2 1.0 SG E:CYS288 2.1 81.8 1.0 SG F:CYS285 2.1 0.3 1.0 SG F:CYS288 2.2 64.1 1.0 CB F:CYS285 3.1 92.3 1.0 CB E:CYS288 3.4 36.9 1.0 CB E:CYS285 3.6 0.1 1.0 CB F:CYS288 3.7 65.3 1.0 N F:CYS288 3.9 69.2 1.0 N E:CYS288 4.0 51.2 1.0 CA E:CYS288 4.2 50.7 1.0 CA F:CYS285 4.3 0.5 1.0 CA F:CYS288 4.3 60.5 1.0 N F:CYS285 4.4 0.0 1.0 CB F:ARG287 4.5 77.2 1.0 CA E:CYS285 4.5 0.6 1.0 N E:CYS285 4.6 0.4 1.0 O E:CYS285 4.7 82.5 1.0 C F:CYS285 4.7 0.2 1.0 C F:ARG287 4.8 75.7 1.0 O F:CYS285 4.9 94.0 1.0 C E:CYS285 4.9 0.1 1.0

Zinc binding site 3 out of 3 in the Crystal Structure of S. Cerevisiae GET3 in the Open State in Complex with GET1 Cytosolic Domain


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Crystal Structure of S. Cerevisiae GET3 in the Open State in Complex with GET1 Cytosolic Domain within 5.0Å range: probe atom residue distance (Å) B Occ I:Zn363 b:0.5 occ:0.50 SG I:CYS285 2.3 1.0 1.0 SG I:CYS288 2.4 99.8 1.0 CB I:CYS285 2.9 0.4 1.0 CB I:CYS288 3.8 91.2 1.0 N I:CYS288 4.1 80.3 1.0 CA I:CYS285 4.1 0.9 1.0 N I:CYS285 4.4 0.9 1.0 CA I:CYS288 4.5 84.5 1.0 C I:CYS285 4.8 0.0 1.0 CB I:ARG287 4.9 87.4 1.0 O I:CYS285 4.9 0.5 1.0 O I:ASN284 4.9 0.5 1.0 C I:ASN284 5.0 0.9 1.0

S.Stefer, S.Reitz, F.Wang, K.Wild, Y.Y.Pang, D.Schwarz, J.Bomke, C.Hein, F.Lohr, F.Bernhard, V.Denic, V.Dotsch, I.Sinning. Structural Basis For Tail-Anchored Membrane Protein Biogenesis By the GET3-Receptor Complex. Science V. 333 758 2011.
ISSN: ISSN 0036-8075
PubMed: 21719644
DOI: 10.1126/SCIENCE.1207125