Zinc in PDB 3seu: Zn-Mediated Polymer of Maltose-Binding Protein A216H/K220H By Synthetic Symmetrization (Form III)

The structure of Zn-Mediated Polymer of Maltose-Binding Protein A216H/K220H By Synthetic Symmetrization (Form III), PDB code: 3seu was solved by M.Zhao, A.B.Soriaga, A.Laganowsky, M.R.Sawaya, D.Cascio, T.O.Yeates, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	56.39 / 1.85
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	49.500, 61.190, 59.760, 90.00, 109.34, 90.00
R / Rfree (%)	16.1 / 19.1

The binding sites of Zinc atom in the Zn-Mediated Polymer of Maltose-Binding Protein A216H/K220H By Synthetic Symmetrization (Form III) (pdb code 3seu). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 8 binding sites of Zinc where determined in the Zn-Mediated Polymer of Maltose-Binding Protein A216H/K220H By Synthetic Symmetrization (Form III), PDB code: 3seu:
Jump to Zinc binding site number: 1; 2; 3; 4; 5; 6; 7; 8;

Zinc binding site 1 out of 8 in the Zn-Mediated Polymer of Maltose-Binding Protein A216H/K220H By Synthetic Symmetrization (Form III)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Zn-Mediated Polymer of Maltose-Binding Protein A216H/K220H By Synthetic Symmetrization (Form III) within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn373 b:20.1 occ:1.00 OE2 A:GLU222 2.0 19.4 1.0 NE2 A:HIS216 2.1 18.3 1.0 CD A:GLU222 2.8 20.0 1.0 OE1 A:GLU222 3.0 17.6 1.0 CD2 A:HIS216 3.1 19.7 1.0 CE1 A:HIS216 3.1 22.8 1.0 ND1 A:HIS216 4.2 18.9 1.0 CG A:HIS216 4.2 19.7 1.0 CG A:GLU222 4.2 20.1 1.0 CG A:HIS220 4.5 20.2 1.0 CB A:HIS220 4.5 16.6 1.0 CD2 A:HIS220 4.7 20.8 1.0 ND1 A:HIS220 4.9 18.3 1.0

Zinc binding site 2 out of 8 in the Zn-Mediated Polymer of Maltose-Binding Protein A216H/K220H By Synthetic Symmetrization (Form III)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Zn-Mediated Polymer of Maltose-Binding Protein A216H/K220H By Synthetic Symmetrization (Form III) within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn374 b:22.2 occ:1.00 NE2 A:HIS204 2.0 19.5 1.0 CD2 A:HIS204 3.0 17.5 1.0 CE1 A:HIS204 3.0 19.3 1.0 CB A:PRO134 4.0 20.1 1.0 ND1 A:HIS204 4.1 16.7 1.0 CG A:HIS204 4.1 18.3 1.0 CB A:ASN202 4.2 21.0 1.0 O A:HOH487 4.3 36.6 1.0 OD1 A:ASN202 4.5 31.5 1.0 CG A:ASN202 4.6 27.1 1.0 CA A:PRO134 4.8 20.8 1.0 O A:PRO134 4.9 17.5 1.0 C A:PRO134 5.0 18.0 1.0

Zinc binding site 3 out of 8 in the Zn-Mediated Polymer of Maltose-Binding Protein A216H/K220H By Synthetic Symmetrization (Form III)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Zn-Mediated Polymer of Maltose-Binding Protein A216H/K220H By Synthetic Symmetrization (Form III) within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn375 b:28.4 occ:1.00 NE2 A:HIS220 2.1 19.6 1.0 O A:ACT384 2.3 42.6 1.0 OXT A:ACT383 2.3 31.3 1.0 O A:HOH428 2.7 39.3 1.0 O A:ACT383 2.9 30.3 1.0 OXT A:ACT384 3.0 41.8 1.0 C A:ACT383 3.0 31.1 1.0 C A:ACT384 3.1 40.8 1.0 CD2 A:HIS220 3.1 20.8 1.0 CE1 A:HIS220 3.1 21.1 1.0 O A:HOH523 4.1 34.5 1.0 ND1 A:HIS220 4.2 18.3 1.0 CG A:HIS220 4.2 20.2 1.0 O A:HOH532 4.5 52.1 1.0 CH3 A:ACT383 4.5 32.2 1.0 CE1 A:HIS216 4.5 22.8 1.0 CH3 A:ACT384 4.5 37.9 1.0 ND1 A:HIS216 4.9 18.9 1.0

Zinc binding site 4 out of 8 in the Zn-Mediated Polymer of Maltose-Binding Protein A216H/K220H By Synthetic Symmetrization (Form III)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Zn-Mediated Polymer of Maltose-Binding Protein A216H/K220H By Synthetic Symmetrization (Form III) within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn376 b:42.5 occ:1.00 OD2 A:ASP208 2.1 36.8 1.0 OD1 A:ASP208 2.2 39.3 1.0 CG A:ASP208 2.4 35.7 1.0 CB A:ASP208 3.9 27.9 1.0 O A:HOH525 4.6 45.8 1.0 CA A:ASP208 4.7 26.9 1.0

Zinc binding site 5 out of 8 in the Zn-Mediated Polymer of Maltose-Binding Protein A216H/K220H By Synthetic Symmetrization (Form III)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 5 of Zn-Mediated Polymer of Maltose-Binding Protein A216H/K220H By Synthetic Symmetrization (Form III) within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn377 b:93.1 occ:1.00 O A:HIS40 2.4 26.6 1.0 ND1 A:HIS40 2.5 32.3 1.0 CE1 A:HIS40 3.3 34.3 1.0 CG A:HIS40 3.6 29.3 1.0 C A:HIS40 3.6 25.4 1.0 O A:HOH418 3.8 29.0 1.0 OD1 A:ASP42 3.9 57.0 1.0 CB A:HIS40 3.9 24.6 1.0 CA A:HIS40 4.4 23.8 1.0 NE2 A:HIS40 4.5 36.3 1.0 N A:PRO41 4.6 26.4 1.0 C A:PRO41 4.6 29.6 1.0 CD2 A:HIS40 4.6 31.7 1.0 N A:ASP42 4.6 32.5 1.0 CA A:GLY14 4.6 23.6 1.0 CA A:PRO41 4.8 31.0 1.0 CG A:ASP42 4.8 54.0 1.0 O A:HOH459 4.9 34.0 1.0 O A:PRO41 5.0 23.6 1.0

Zinc binding site 6 out of 8 in the Zn-Mediated Polymer of Maltose-Binding Protein A216H/K220H By Synthetic Symmetrization (Form III)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 6 of Zn-Mediated Polymer of Maltose-Binding Protein A216H/K220H By Synthetic Symmetrization (Form III) within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn378 b:52.2 occ:0.50 OE2 A:GLU275 2.8 37.6 0.5 CD A:GLU275 2.8 36.0 0.5 OE1 A:GLU275 3.0 36.3 0.5 O A:HOH553 3.0 47.4 1.0 CG A:GLU275 3.6 33.2 0.5 CG A:GLU275 4.1 31.3 0.5 OE1 A:GLU275 4.9 37.2 0.5 CB A:GLU275 5.0 29.2 0.5

Zinc binding site 7 out of 8 in the Zn-Mediated Polymer of Maltose-Binding Protein A216H/K220H By Synthetic Symmetrization (Form III)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 7 of Zn-Mediated Polymer of Maltose-Binding Protein A216H/K220H By Synthetic Symmetrization (Form III) within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn379 b:0.5 occ:1.00 O A:HOH503 2.4 59.7 1.0 OD2 A:ASP83 2.4 33.3 1.0 OD1 A:ASP83 3.1 31.4 1.0 CG A:ASP83 3.1 32.5 1.0 CB A:ALA85 3.8 26.8 1.0 CB A:ASP83 4.6 31.2 1.0 O A:HOH502 4.6 32.0 1.0 N A:PHE86 4.7 23.3 1.0 O A:HOH425 4.7 22.6 1.0 O A:HOH529 4.8 30.9 1.0 CA A:ALA85 4.9 26.9 1.0

Zinc binding site 8 out of 8 in the Zn-Mediated Polymer of Maltose-Binding Protein A216H/K220H By Synthetic Symmetrization (Form III)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 8 of Zn-Mediated Polymer of Maltose-Binding Protein A216H/K220H By Synthetic Symmetrization (Form III) within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn380 b:0.4 occ:1.00 OE2 A:GLU39 2.3 45.1 1.0 OXT A:ACT386 2.4 65.0 1.0 CD A:GLU39 3.3 38.7 1.0 C A:ACT386 3.5 63.5 1.0 OE1 A:GLU39 3.7 38.0 1.0 O A:HOH545 3.7 61.4 1.0 O A:ACT386 3.8 64.4 1.0 CG A:GLU39 4.5 31.1 1.0 CH3 A:ACT386 4.8 60.8 1.0

A.Laganowsky, M.Zhao, A.B.Soriaga, M.R.Sawaya, D.Cascio, T.O.Yeates. An Approach to Crystallizing Proteins By Metal-Mediated Synthetic Symmetrization. Protein Sci. V. 20 1876 2011.
ISSN: ISSN 0961-8368
PubMed: 21898649
DOI: 10.1002/PRO.727