Zinc in PDB 3osl: Structure of Bovine Thrombin-Activatable Fibrinolysis Inhibitor in Complex with Tick Carboxypeptidase Inhibitor

All present enzymatic activity of Structure of Bovine Thrombin-Activatable Fibrinolysis Inhibitor in Complex with Tick Carboxypeptidase Inhibitor:
3.4.17.20;

The structure of Structure of Bovine Thrombin-Activatable Fibrinolysis Inhibitor in Complex with Tick Carboxypeptidase Inhibitor, PDB code: 3osl was solved by Z.Valnickova, L.Sanglas, J.L.Arolas, S.V.Petersen, C.Schar, D.Otzen, F.X.Aviles, F.X.Gomis-Ruth, J.J.Enghild, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 6.00
Space group	P 41 3 2
Cell size a, b, c (Å), α, β, γ (°)	279.100, 279.100, 279.100, 90.00, 90.00, 90.00
R / Rfree (%)	31.3 / 32

The binding sites of Zinc atom in the Structure of Bovine Thrombin-Activatable Fibrinolysis Inhibitor in Complex with Tick Carboxypeptidase Inhibitor (pdb code 3osl). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Structure of Bovine Thrombin-Activatable Fibrinolysis Inhibitor in Complex with Tick Carboxypeptidase Inhibitor, PDB code: 3osl:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Structure of Bovine Thrombin-Activatable Fibrinolysis Inhibitor in Complex with Tick Carboxypeptidase Inhibitor


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Structure of Bovine Thrombin-Activatable Fibrinolysis Inhibitor in Complex with Tick Carboxypeptidase Inhibitor within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn999 b:22.2 occ:1.00 OE2 A:GLU184 2.0 21.3 1.0 ND1 A:HIS310 2.1 21.3 1.0 ND1 A:HIS181 2.1 19.0 1.0 O B:LEU74 2.4 17.2 1.0 C B:LEU74 2.5 17.5 1.0 CD A:GLU184 2.7 21.6 1.0 OE1 A:GLU184 2.8 21.5 1.0 CE1 A:HIS310 2.9 20.9 1.0 CE1 A:HIS181 3.0 21.7 1.0 CG A:HIS181 3.2 20.0 1.0 CG A:HIS310 3.2 20.5 1.0 CB A:HIS181 3.5 21.2 1.0 CB A:HIS310 3.6 20.6 1.0 CA B:LEU74 4.0 16.7 1.0 NH1 A:ARG239 4.0 23.6 1.0 NE2 A:HIS310 4.1 19.6 1.0 NE2 A:HIS181 4.1 20.9 1.0 CG A:GLU184 4.2 22.1 1.0 CD2 A:HIS310 4.2 18.6 1.0 CD2 A:HIS181 4.2 20.7 1.0 O A:SER311 4.3 24.2 1.0 CA A:HIS310 4.5 20.1 1.0 N A:SER311 4.8 22.3 1.0 OE2 A:GLU385 4.8 26.2 1.0 CB B:LEU74 4.9 16.3 1.0 N B:LEU74 4.9 16.4 1.0 CA A:HIS181 4.9 20.2 1.0 CG2 A:ILE180 4.9 23.2 1.0 CZ A:ARG239 4.9 25.6 1.0

Zinc binding site 2 out of 2 in the Structure of Bovine Thrombin-Activatable Fibrinolysis Inhibitor in Complex with Tick Carboxypeptidase Inhibitor


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Structure of Bovine Thrombin-Activatable Fibrinolysis Inhibitor in Complex with Tick Carboxypeptidase Inhibitor within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn999 b:20.9 occ:1.00 OE2 C:GLU184 2.0 19.8 1.0 ND1 C:HIS310 2.1 20.4 1.0 ND1 C:HIS181 2.1 17.6 1.0 O D:LEU74 2.3 40.6 1.0 C D:LEU74 2.5 40.9 1.0 CD C:GLU184 2.7 20.2 1.0 OE1 C:GLU184 2.8 20.2 1.0 CE1 C:HIS310 2.9 20.0 1.0 CE1 C:HIS181 3.0 20.3 1.0 CG C:HIS181 3.2 18.6 1.0 CG C:HIS310 3.2 19.8 1.0 CB C:HIS181 3.5 19.7 1.0 CB C:HIS310 3.6 19.8 1.0 CA D:LEU74 4.0 40.2 1.0 NH1 C:ARG239 4.0 21.8 1.0 NE2 C:HIS310 4.1 18.9 1.0 NE2 C:HIS181 4.2 19.4 1.0 CG C:GLU184 4.2 20.6 1.0 CD2 C:HIS310 4.2 18.1 1.0 CD2 C:HIS181 4.2 19.2 1.0 O C:SER311 4.3 22.8 1.0 CA C:HIS310 4.5 19.4 1.0 N C:SER311 4.8 21.3 1.0 OE2 C:GLU385 4.8 25.3 1.0 N D:LEU74 4.8 40.4 1.0 CA C:HIS181 4.9 18.8 1.0 CG2 C:ILE180 4.9 22.4 1.0 CB D:LEU74 4.9 40.2 1.0 CZ C:ARG239 4.9 23.7 1.0

Z.Valnickova, L.Sanglas, J.L.Arolas, S.V.Petersen, C.Schar, D.Otzen, F.X.Aviles, F.X.Gomis-Ruth, J.J.Enghild. Flexibility of the Thrombin-Activatable Fibrinolysis Inhibitor Pro-Domain Enables Productive Binding of Protein Substrates. J.Biol.Chem. V. 285 38243 2010.
ISSN: ISSN 0021-9258
PubMed: 20880845
DOI: 10.1074/JBC.M110.150342