Zinc in PDB 3o8r: Visualizing Atp-Dependent Rna Translocation By the NS3 Helicase From Hcv

All present enzymatic activity of Visualizing Atp-Dependent Rna Translocation By the NS3 Helicase From Hcv:
3.4.21.98; 3.6.1.15; 3.6.4.13;

The structure of Visualizing Atp-Dependent Rna Translocation By the NS3 Helicase From Hcv, PDB code: 3o8r was solved by T.C.Appleby, J.R.Somoza, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	43.68 / 2.30
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	91.033, 110.827, 141.979, 90.00, 90.00, 90.00
R / Rfree (%)	21.5 / 26.3

The structure of Visualizing Atp-Dependent Rna Translocation By the NS3 Helicase From Hcv also contains other interesting chemical elements:

Fluorine	(F)	3 atoms
Magnesium	(Mg)	1 atom
Bromine	(Br)	1 atom

The binding sites of Zinc atom in the Visualizing Atp-Dependent Rna Translocation By the NS3 Helicase From Hcv (pdb code 3o8r). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Visualizing Atp-Dependent Rna Translocation By the NS3 Helicase From Hcv, PDB code: 3o8r:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Visualizing Atp-Dependent Rna Translocation By the NS3 Helicase From Hcv


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Visualizing Atp-Dependent Rna Translocation By the NS3 Helicase From Hcv within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn800 b:61.1 occ:1.00 SG A:CYS97 2.4 59.2 1.0 SG A:CYS145 2.4 50.0 1.0 SG A:CYS99 2.5 67.8 1.0 CD2 A:HIS149 2.9 52.2 1.0 CB A:CYS97 3.1 55.0 1.0 CB A:CYS145 3.1 49.8 1.0 CB A:CYS99 3.5 67.7 1.0 CA A:CYS97 3.6 56.1 1.0 N A:CYS99 3.6 67.3 1.0 OG A:SER147 3.7 53.3 1.0 CG A:HIS149 3.7 56.7 1.0 N A:THR98 3.8 63.9 1.0 NE2 A:HIS149 3.9 58.5 1.0 CB A:HIS149 4.0 53.4 1.0 C A:CYS97 4.0 57.6 1.0 CA A:CYS99 4.1 68.7 1.0 N A:GLY100 4.6 64.5 1.0 CA A:CYS145 4.6 44.9 1.0 C A:CYS99 4.7 69.2 1.0 C A:THR98 4.7 68.1 1.0 ND1 A:HIS149 4.8 54.7 1.0 CB A:SER147 4.8 50.9 1.0 CB A:SER101 4.9 59.8 1.0 CE1 A:HIS149 4.9 53.3 1.0 CA A:THR98 4.9 65.0 1.0 N A:CYS97 4.9 46.8 1.0 N A:SER101 4.9 65.8 1.0

Zinc binding site 2 out of 2 in the Visualizing Atp-Dependent Rna Translocation By the NS3 Helicase From Hcv


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Visualizing Atp-Dependent Rna Translocation By the NS3 Helicase From Hcv within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn800 b:61.4 occ:1.00 SG B:CYS145 2.4 54.2 1.0 SG B:CYS99 2.4 62.5 1.0 SG B:CYS97 2.6 55.4 1.0 ND1 B:HIS149 3.0 59.5 1.0 CB B:CYS99 3.1 57.1 1.0 CB B:CYS145 3.2 50.5 1.0 CB B:CYS97 3.4 47.8 1.0 N B:CYS99 3.6 62.4 1.0 CB B:HIS149 3.7 55.2 1.0 CG B:HIS149 3.7 60.1 1.0 CA B:CYS97 3.8 57.0 1.0 CA B:CYS99 3.9 65.4 1.0 O B:HOH738 3.9 54.1 1.0 CE1 B:HIS149 4.0 65.6 1.0 N B:THR98 4.1 60.6 1.0 C B:CYS97 4.3 59.9 1.0 CB B:SER147 4.4 52.8 1.0 OG B:SER147 4.5 56.7 1.0 CA B:CYS145 4.6 52.2 1.0 C B:CYS99 4.7 61.4 1.0 N B:HIS149 4.7 51.1 1.0 CA B:HIS149 4.8 51.0 1.0 N B:GLY100 4.8 62.4 1.0 C B:THR98 4.8 68.0 1.0 CD2 B:HIS149 4.9 55.5 1.0 N B:SER147 5.0 52.4 1.0

T.C.Appleby, R.Anderson, O.Fedorova, A.M.Pyle, R.Wang, X.Liu, K.M.Brendza, J.R.Somoza. Visualizing Atp-Dependent Rna Translocation By the NS3 Helicase From Hcv. J.Mol.Biol. V. 405 1139 2011.
ISSN: ISSN 0022-2836
PubMed: 21145896
DOI: 10.1016/J.JMB.2010.11.034