Zinc in PDB 3o8b: Visualizing Atp-Dependent Rna Translocation By the NS3 Helicase From Hcv

All present enzymatic activity of Visualizing Atp-Dependent Rna Translocation By the NS3 Helicase From Hcv:
3.4.21.98; 3.6.1.15; 3.6.4.13;

The structure of Visualizing Atp-Dependent Rna Translocation By the NS3 Helicase From Hcv, PDB code: 3o8b was solved by T.C.Appleby, J.R.Somoza, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 1.95
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	91.299, 111.955, 139.927, 90.00, 90.00, 90.00
R / Rfree (%)	18.3 / 22.2

The binding sites of Zinc atom in the Visualizing Atp-Dependent Rna Translocation By the NS3 Helicase From Hcv (pdb code 3o8b). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Visualizing Atp-Dependent Rna Translocation By the NS3 Helicase From Hcv, PDB code: 3o8b:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Visualizing Atp-Dependent Rna Translocation By the NS3 Helicase From Hcv


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Visualizing Atp-Dependent Rna Translocation By the NS3 Helicase From Hcv within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn800 b:19.9 occ:1.00 SG A:CYS97 2.3 17.3 1.0 SG A:CYS145 2.3 15.6 1.0 SG A:CYS99 2.4 19.0 1.0 O A:HOH2 2.4 3.9 1.0 CB A:CYS145 3.0 14.4 1.0 CB A:CYS97 3.2 18.8 1.0 CB A:CYS99 3.3 22.6 1.0 N A:CYS99 3.5 17.5 1.0 CA A:CYS97 3.8 19.0 1.0 CA A:CYS99 3.9 21.5 1.0 N A:THR98 4.0 18.6 1.0 O A:HOH807 4.1 28.3 1.0 C A:CYS97 4.2 17.2 1.0 N A:GLY100 4.4 25.7 1.0 CB A:SER147 4.5 19.2 1.0 CA A:CYS145 4.5 15.5 1.0 C A:CYS99 4.5 25.4 1.0 CB A:HIS149 4.6 13.5 1.0 OG A:SER147 4.6 27.9 1.0 C A:THR98 4.7 20.2 1.0 O A:HOH1073 4.8 27.8 1.0 N A:SER147 4.9 16.9 1.0 CA A:THR98 4.9 19.5 1.0 C A:CYS145 5.0 16.4 1.0 N A:SER101 5.0 22.6 1.0 CD A:PRO146 5.0 16.8 1.0

Zinc binding site 2 out of 2 in the Visualizing Atp-Dependent Rna Translocation By the NS3 Helicase From Hcv


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Visualizing Atp-Dependent Rna Translocation By the NS3 Helicase From Hcv within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn800 b:45.6 occ:1.00 SG B:CYS99 2.4 49.7 1.0 SG B:CYS145 2.4 37.3 1.0 O B:HOH824 2.5 37.0 1.0 SG B:CYS97 2.5 43.9 1.0 CB B:CYS145 3.1 35.7 1.0 O B:HOH1041 3.1 45.1 1.0 CB B:CYS97 3.3 38.3 1.0 CB B:CYS99 3.5 48.8 1.0 N B:CYS99 3.6 48.9 1.0 CA B:CYS97 3.7 41.7 1.0 N B:THR98 3.8 46.4 1.0 ND1 B:HIS149 3.9 49.2 1.0 CB B:HIS149 4.0 35.7 1.0 CA B:CYS99 4.1 48.0 1.0 C B:CYS97 4.1 42.8 1.0 CG B:HIS149 4.4 42.4 1.0 CB B:SER147 4.5 41.3 1.0 CA B:CYS145 4.6 33.6 1.0 N B:GLY100 4.6 47.8 1.0 OG B:SER147 4.7 45.0 1.0 C B:THR98 4.7 48.2 1.0 C B:CYS99 4.8 49.5 1.0 CA B:THR98 4.9 48.8 1.0 CB B:SER101 4.9 45.0 1.0 CE1 B:HIS149 5.0 50.1 1.0 N B:SER147 5.0 38.9 1.0

T.C.Appleby, R.Anderson, O.Fedorova, A.M.Pyle, R.Wang, X.Liu, K.M.Brendza, J.R.Somoza. Visualizing Atp-Dependent Rna Translocation By the NS3 Helicase From Hcv. J.Mol.Biol. V. 405 1139 2011.
ISSN: ISSN 0022-2836
PubMed: 21145896
DOI: 10.1016/J.JMB.2010.11.034