Zinc in PDB 3jue: Crystal Structure of Arfgap and Ank Repeat Domain of ACAP1

The structure of Crystal Structure of Arfgap and Ank Repeat Domain of ACAP1, PDB code: 3jue was solved by X.Pang, K.Zhang, J.Ma, Q.Zhou, F.Sun, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	45.00 / 2.30
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	107.809, 163.473, 41.092, 90.00, 90.00, 90.00
R / Rfree (%)	20.2 / 21.9

The binding sites of Zinc atom in the Crystal Structure of Arfgap and Ank Repeat Domain of ACAP1 (pdb code 3jue). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of Arfgap and Ank Repeat Domain of ACAP1, PDB code: 3jue:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of Arfgap and Ank Repeat Domain of ACAP1


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Arfgap and Ank Repeat Domain of ACAP1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn999 b:38.5 occ:1.00 SG A:CYS443 2.4 32.0 1.0 SG A:CYS423 2.4 31.0 1.0 SG A:CYS440 2.4 30.6 1.0 SG A:CYS420 2.4 29.9 1.0 CB A:CYS443 3.1 28.3 1.0 CB A:CYS420 3.3 29.9 1.0 CB A:CYS423 3.4 32.3 1.0 CB A:CYS440 3.7 30.4 1.0 N A:CYS423 3.8 29.1 1.0 N A:CYS443 4.0 30.8 1.0 CA A:CYS423 4.1 31.5 1.0 N A:CYS440 4.2 27.2 1.0 CA A:CYS443 4.2 29.1 1.0 CB A:GLU425 4.3 34.6 1.0 O A:HOH1065 4.4 36.7 1.0 NZ A:LYS506 4.5 35.8 1.0 CA A:CYS440 4.5 29.1 1.0 C A:ASP422 4.6 29.1 1.0 C A:CYS423 4.7 32.9 1.0 CA A:CYS420 4.7 29.9 1.0 CD A:LYS506 4.8 34.8 1.0 CB A:ASP422 4.8 27.1 1.0 N A:ASP422 4.8 27.2 1.0 N A:GLU425 4.8 33.8 1.0 N A:ARG424 4.9 31.7 1.0 CA A:ASP422 4.9 27.6 1.0 CE A:LYS506 5.0 37.7 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of Arfgap and Ank Repeat Domain of ACAP1


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Arfgap and Ank Repeat Domain of ACAP1 within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn999 b:37.5 occ:1.00 SG B:CYS443 2.3 29.0 1.0 SG B:CYS420 2.3 23.6 1.0 SG B:CYS423 2.4 28.2 1.0 SG B:CYS440 2.4 30.7 1.0 CB B:CYS443 3.1 29.7 1.0 CB B:CYS420 3.3 24.6 1.0 CB B:CYS423 3.3 27.2 1.0 CB B:CYS440 3.5 28.4 1.0 N B:CYS423 3.7 27.2 1.0 CA B:CYS423 4.0 27.4 1.0 N B:CYS440 4.0 25.9 1.0 N B:CYS443 4.2 32.2 1.0 CA B:CYS443 4.2 31.3 1.0 CB B:GLU425 4.3 28.1 1.0 CA B:CYS440 4.3 28.4 1.0 O B:HOH1069 4.4 33.7 1.0 NZ B:LYS506 4.5 30.4 1.0 C B:ASP422 4.6 28.1 1.0 C B:CYS423 4.6 27.5 1.0 CD B:LYS506 4.7 29.9 1.0 CA B:CYS420 4.7 25.0 1.0 N B:GLU425 4.7 26.5 1.0 CB B:ASP422 4.7 27.9 1.0 N B:ARG424 4.7 27.0 1.0 N B:ASP422 4.9 26.4 1.0 CA B:ASP422 4.9 27.2 1.0 CE B:LYS506 4.9 31.0 1.0 C B:CYS440 5.0 29.7 1.0

M.Bai, X.Pang, J.Lou, Q.Zhou, K.Zhang, J.Ma, J.Li, F.Sun, V.W.Hsu. Mechanistic Insights Into Regulated Cargo Binding By ACAP1 Protein J.Biol.Chem. V. 287 28675 2012.
ISSN: ISSN 0021-9258
PubMed: 22645133
DOI: 10.1074/JBC.M112.378810