Zinc in PDB 3im4: Crystal Structure of Camp-Dependent Protein Kinase A Regulatory Subunit I Alpha in Complex with Dual-Specific A- Kinase Anchoring Protein 2

The structure of Crystal Structure of Camp-Dependent Protein Kinase A Regulatory Subunit I Alpha in Complex with Dual-Specific A- Kinase Anchoring Protein 2, PDB code: 3im4 was solved by G.N.Sarma, F.S.Kinderman, C.Kim, S.Von Daake, S.S.Taylor, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	39.96 / 2.29
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	40.400, 56.020, 56.990, 90.00, 90.00, 90.00
R / Rfree (%)	18.6 / 25.4

The binding sites of Zinc atom in the Crystal Structure of Camp-Dependent Protein Kinase A Regulatory Subunit I Alpha in Complex with Dual-Specific A- Kinase Anchoring Protein 2 (pdb code 3im4). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 5 binding sites of Zinc where determined in the Crystal Structure of Camp-Dependent Protein Kinase A Regulatory Subunit I Alpha in Complex with Dual-Specific A- Kinase Anchoring Protein 2, PDB code: 3im4:
Jump to Zinc binding site number: 1; 2; 3; 4; 5;

Zinc binding site 1 out of 5 in the Crystal Structure of Camp-Dependent Protein Kinase A Regulatory Subunit I Alpha in Complex with Dual-Specific A- Kinase Anchoring Protein 2


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Camp-Dependent Protein Kinase A Regulatory Subunit I Alpha in Complex with Dual-Specific A- Kinase Anchoring Protein 2 within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn701 b:55.3 occ:0.40 NE2 A:HIS23 2.2 47.4 1.0 CD2 A:HIS23 2.9 38.0 1.0 CE1 A:HIS23 3.4 52.3 1.0 ZN A:ZN705 3.9 55.4 0.6 CG A:HIS23 4.1 43.6 1.0 O B:GLU42 4.3 50.6 1.0 ND1 A:HIS23 4.3 49.3 1.0 CA B:GLU42 4.5 41.9 1.0 O B:PRO41 4.6 35.2 1.0 C B:GLU42 4.8 48.9 1.0 CE1 A:TYR19 4.9 37.1 1.0

Zinc binding site 2 out of 5 in the Crystal Structure of Camp-Dependent Protein Kinase A Regulatory Subunit I Alpha in Complex with Dual-Specific A- Kinase Anchoring Protein 2


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Camp-Dependent Protein Kinase A Regulatory Subunit I Alpha in Complex with Dual-Specific A- Kinase Anchoring Protein 2 within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn702 b:75.1 occ:0.60 OE2 A:GLU15 2.5 74.1 1.0 O A:HOH127 2.7 56.3 1.0 CD A:GLU15 3.3 71.6 1.0 OE1 A:GLU15 3.3 66.6 1.0 NH2 B:ARG40 4.5 64.9 1.0 OG A:SER12 4.5 0.3 1.0 CG A:GLU15 4.7 65.0 1.0

Zinc binding site 3 out of 5 in the Crystal Structure of Camp-Dependent Protein Kinase A Regulatory Subunit I Alpha in Complex with Dual-Specific A- Kinase Anchoring Protein 2


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Crystal Structure of Camp-Dependent Protein Kinase A Regulatory Subunit I Alpha in Complex with Dual-Specific A- Kinase Anchoring Protein 2 within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn705 b:55.4 occ:0.60 O A:HOH103 2.2 26.5 1.0 CE1 A:HIS23 2.3 52.3 1.0 NE2 A:HIS23 3.0 47.4 1.0 ND1 A:HIS23 3.5 49.3 1.0 CD1 A:TYR19 3.9 32.0 1.0 ZN A:ZN701 3.9 55.3 0.4 CE1 A:TYR19 4.3 37.1 1.0 CD2 A:HIS23 4.3 38.0 1.0 CB A:LYS22 4.4 47.5 1.0 CG A:HIS23 4.5 43.6 1.0 CG A:TYR19 4.8 32.7 1.0 O A:HOH122 4.9 50.7 1.0 CA A:TYR19 4.9 37.3 1.0

Zinc binding site 4 out of 5 in the Crystal Structure of Camp-Dependent Protein Kinase A Regulatory Subunit I Alpha in Complex with Dual-Specific A- Kinase Anchoring Protein 2


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Crystal Structure of Camp-Dependent Protein Kinase A Regulatory Subunit I Alpha in Complex with Dual-Specific A- Kinase Anchoring Protein 2 within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn703 b:46.8 occ:1.00 OE2 B:GLU42 2.1 49.4 1.0 O B:HOH101 2.1 15.4 1.0 OE1 B:GLU42 2.5 54.5 1.0 CD B:GLU42 2.6 52.1 1.0 CG B:GLU42 4.1 52.7 1.0 NH1 B:ARG43 4.2 95.7 1.0 NH2 B:ARG43 4.2 96.6 1.0 CZ B:ARG43 4.7 95.1 1.0

Zinc binding site 5 out of 5 in the Crystal Structure of Camp-Dependent Protein Kinase A Regulatory Subunit I Alpha in Complex with Dual-Specific A- Kinase Anchoring Protein 2


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 5 of Crystal Structure of Camp-Dependent Protein Kinase A Regulatory Subunit I Alpha in Complex with Dual-Specific A- Kinase Anchoring Protein 2 within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn704 b:75.9 occ:0.80 OE2 B:GLU15 2.7 76.9 1.0 OE1 B:GLU15 2.9 68.1 1.0 CD B:GLU15 3.2 73.5 1.0 CG B:GLU15 4.6 70.0 1.0 NH2 A:ARG40 4.7 59.7 1.0 N B:SER12 4.7 74.1 1.0

G.N.Sarma, F.S.Kinderman, C.Kim, S.Von Daake, L.Chen, B.C.Wang, S.S.Taylor. Structure of D-AKAP2:Pka Ri Complex: Insights Into Akap Specificity and Selectivity Structure V. 18 155 2010.
ISSN: ISSN 0969-2126
PubMed: 20159461
DOI: 10.1016/J.STR.2009.12.012