Zinc in PDB 3h1k: Chicken Cytochrome BC1 Complex with Zn++ and An Iodinated Derivative of Kresoxim-Methyl Bound

All present enzymatic activity of Chicken Cytochrome BC1 Complex with Zn++ and An Iodinated Derivative of Kresoxim-Methyl Bound:
1.10.2.2;

The structure of Chicken Cytochrome BC1 Complex with Zn++ and An Iodinated Derivative of Kresoxim-Methyl Bound, PDB code: 3h1k was solved by E.A.Berry, Z.Zhang, H.D.Bellamy, L.S.Huang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	18.00 / 3.48
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	171.717, 181.297, 241.288, 90.00, 90.00, 90.00
R / Rfree (%)	23.9 / 28.4

The structure of Chicken Cytochrome BC1 Complex with Zn++ and An Iodinated Derivative of Kresoxim-Methyl Bound also contains other interesting chemical elements:

Iodine	(I)	2 atoms
Iron	(Fe)	10 atoms

The binding sites of Zinc atom in the Chicken Cytochrome BC1 Complex with Zn++ and An Iodinated Derivative of Kresoxim-Methyl Bound (pdb code 3h1k). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Chicken Cytochrome BC1 Complex with Zn++ and An Iodinated Derivative of Kresoxim-Methyl Bound, PDB code: 3h1k:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Chicken Cytochrome BC1 Complex with Zn++ and An Iodinated Derivative of Kresoxim-Methyl Bound


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Chicken Cytochrome BC1 Complex with Zn++ and An Iodinated Derivative of Kresoxim-Methyl Bound within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn2012 b:0.5 occ:1.00 OE1 C:GLU255 2.2 96.3 1.0 NE2 D:HIS121 2.4 78.5 1.0 NE2 C:HIS268 2.5 99.2 1.0 OD2 C:ASP253 2.6 84.1 1.0 CE1 C:HIS268 2.6 99.6 1.0 OD1 C:ASP253 2.9 85.8 1.0 CG C:ASP253 3.0 85.8 1.0 CE1 D:HIS121 3.1 78.4 1.0 CD C:GLU255 3.2 91.4 1.0 CD2 D:HIS121 3.5 77.5 1.0 CG C:GLU255 3.5 88.6 1.0 CD C:PRO254 3.7 83.9 1.0 CD2 C:HIS268 3.7 97.8 1.0 CG C:PRO254 3.7 83.5 1.0 ND1 C:HIS268 3.9 99.0 1.0 ND1 D:HIS121 4.2 77.7 1.0 OE2 C:GLU255 4.4 91.2 1.0 CG C:HIS268 4.4 96.2 1.0 CB C:ASP253 4.4 87.6 1.0 CG D:HIS121 4.4 75.8 1.0 N C:PRO254 4.5 84.4 1.0 N C:GLU255 4.6 77.1 1.0 CB C:GLU255 4.9 80.4 1.0 CA C:ASP253 5.0 87.3 1.0

Zinc binding site 2 out of 2 in the Chicken Cytochrome BC1 Complex with Zn++ and An Iodinated Derivative of Kresoxim-Methyl Bound


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Chicken Cytochrome BC1 Complex with Zn++ and An Iodinated Derivative of Kresoxim-Methyl Bound within 5.0Å range: probe atom residue distance (Å) B Occ P:Zn3012 b:0.1 occ:1.00 OE1 P:GLU255 2.1 0.6 1.0 NE2 P:HIS268 2.2 0.0 1.0 CE1 P:HIS268 2.3 0.4 1.0 NE2 Q:HIS121 2.3 0.6 1.0 OD2 P:ASP253 2.8 0.6 1.0 CD P:GLU255 3.1 0.8 1.0 OD1 P:ASP253 3.1 0.1 1.0 CE1 Q:HIS121 3.1 1.0 1.0 CG P:ASP253 3.3 0.5 1.0 CD2 Q:HIS121 3.4 0.0 1.0 CG P:GLU255 3.4 0.4 1.0 CD2 P:HIS268 3.4 0.1 1.0 ND1 P:HIS268 3.5 0.9 1.0 CG P:PRO254 4.0 0.5 1.0 CD P:PRO254 4.0 0.0 1.0 CG P:HIS268 4.1 0.5 1.0 OE2 P:GLU255 4.3 0.2 1.0 ND1 Q:HIS121 4.3 0.1 1.0 CG Q:HIS121 4.4 0.7 1.0 CB P:ASP253 4.7 0.1 1.0 N P:GLU255 4.8 0.1 1.0 CB P:GLU255 4.8 0.6 1.0 N P:PRO254 4.9 0.9 1.0 O P:HIS268 5.0 0.2 1.0

E.A.Berry, Z.Zhang, H.D.Bellamy, L.Huang. Crystallographic Location of Two Zn(2+)-Binding Sites in the Avian Cytochrome Bc(1) Complex Biochim.Biophys.Acta V.1459 440 2000.
ISSN: ISSN 0006-3002
PubMed: 11004461