Zinc in PDB 3guc: Human Ubiquitin-Activating Enzyme 5 in Complex with Amppnp

The structure of Human Ubiquitin-Activating Enzyme 5 in Complex with Amppnp, PDB code: 3guc was solved by J.R.Walker, J.P.Bacik, Y.Li, J.Weigelt, C.Bountra, C.H.Arrowsmith, A.M.Edwards, A.Bochkarev, S.Dhe-Paganon, Structural Genomics Consortium(Sgc), with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	40.82 / 2.25
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	77.394, 77.394, 205.892, 90.00, 90.00, 120.00
R / Rfree (%)	20.3 / 25.5

The binding sites of Zinc atom in the Human Ubiquitin-Activating Enzyme 5 in Complex with Amppnp (pdb code 3guc). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Human Ubiquitin-Activating Enzyme 5 in Complex with Amppnp, PDB code: 3guc:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Human Ubiquitin-Activating Enzyme 5 in Complex with Amppnp


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Human Ubiquitin-Activating Enzyme 5 in Complex with Amppnp within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn401 b:27.8 occ:1.00 SG A:CYS308 2.3 43.0 1.0 SG A:CYS226 2.4 42.1 1.0 SG A:CYS229 2.4 46.1 1.0 SG A:CYS303 2.4 45.6 1.0 CB A:CYS303 3.0 41.5 1.0 CB A:CYS308 3.1 40.2 1.0 CB A:CYS226 3.3 40.7 1.0 N A:CYS229 3.5 43.3 1.0 CB A:CYS229 3.5 44.6 1.0 CA A:CYS229 3.6 44.3 1.0 N A:CYS226 3.9 40.8 1.0 CA A:CYS226 4.1 40.2 1.0 C A:ALA228 4.2 42.6 1.0 O A:HOH415 4.5 28.9 1.0 CA A:CYS303 4.5 45.7 1.0 O A:CYS226 4.5 40.7 1.0 C A:CYS226 4.5 40.4 1.0 CA A:CYS308 4.6 42.9 1.0 N A:ALA228 4.7 41.3 1.0 OE1 A:GLN312 4.7 85.4 1.0 CB A:ALA228 4.7 42.5 1.0 CA A:ALA228 4.8 41.8 1.0 ND2 A:ASN196 4.8 36.3 1.0 CB A:ASP305 4.9 51.9 1.0 O A:ALA228 4.9 43.5 1.0 O A:ASN300 4.9 42.3 1.0

Zinc binding site 2 out of 2 in the Human Ubiquitin-Activating Enzyme 5 in Complex with Amppnp


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Human Ubiquitin-Activating Enzyme 5 in Complex with Amppnp within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn401 b:27.1 occ:1.00 SG B:CYS308 2.3 37.8 1.0 SG B:CYS303 2.3 41.0 1.0 SG B:CYS229 2.4 45.4 1.0 SG B:CYS226 2.4 42.5 1.0 CB B:CYS308 3.1 41.0 1.0 CB B:CYS303 3.1 41.7 1.0 CB B:CYS226 3.4 40.7 1.0 N B:CYS229 3.5 41.9 1.0 CB B:CYS229 3.5 41.6 1.0 CA B:CYS229 3.6 44.0 1.0 N B:CYS226 4.0 39.8 1.0 CA B:CYS226 4.2 38.1 1.0 C B:ALA228 4.2 42.6 1.0 O B:HOH420 4.5 24.7 1.0 CA B:CYS308 4.6 43.4 1.0 C B:CYS226 4.6 39.8 1.0 CA B:CYS303 4.6 45.6 1.0 CB B:ALA228 4.6 40.7 1.0 O B:CYS226 4.6 39.4 1.0 N B:ALA228 4.7 41.4 1.0 ND2 B:ASN196 4.7 39.0 1.0 CA B:ALA228 4.7 42.2 1.0 OE1 B:GLN312 4.8 53.0 1.0 CB B:ASP305 4.8 48.0 1.0 O B:ALA228 4.9 45.1 1.0 O B:ASN300 5.0 45.0 1.0

J.R.Walker, J.P.Bacik, Y.Li, J.Weigelt, C.Bountra, C.H.Arrowsmith, A.M.Edwards, A.Bochkarev, S.Dhe-Paganon. Human Ubiquitin-Activating Enzyme 5 in Complex with Amppnp To Be Published.