Zinc in PDB 3fm8: Crystal Structure of Full Length Centaurin Alpha-1 Bound with the Fha Domain of KIF13B (Capri Target)

The structure of Crystal Structure of Full Length Centaurin Alpha-1 Bound with the Fha Domain of KIF13B (Capri Target), PDB code: 3fm8 was solved by L.Shen, Y.Tong, W.Tempel, F.Mackenzie, C.H.Arrowsmith, A.M.Edwards, C.Bountra, J.Weigelt, A.Bochkarev, H.Park, Structural Genomicsconsortium (Sgc), with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.87 / 2.30
Space group	P 41 21 2
Cell size a, b, c (Å), α, β, γ (°)	115.632, 115.632, 189.291, 90.00, 90.00, 90.00
R / Rfree (%)	23.1 / 27.2

The binding sites of Zinc atom in the Crystal Structure of Full Length Centaurin Alpha-1 Bound with the Fha Domain of KIF13B (Capri Target) (pdb code 3fm8). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of Full Length Centaurin Alpha-1 Bound with the Fha Domain of KIF13B (Capri Target), PDB code: 3fm8:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of Full Length Centaurin Alpha-1 Bound with the Fha Domain of KIF13B (Capri Target)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Full Length Centaurin Alpha-1 Bound with the Fha Domain of KIF13B (Capri Target) within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn401 b:25.5 occ:1.00 SG C:CYS24 2.3 26.4 1.0 SG C:CYS44 2.3 25.3 1.0 SG C:CYS21 2.3 21.6 1.0 SG C:CYS41 2.4 24.3 1.0 CB C:CYS44 3.3 23.9 1.0 CB C:CYS21 3.3 21.0 1.0 CB C:CYS24 3.3 24.9 1.0 CB C:CYS41 3.5 24.6 1.0 N C:CYS24 3.7 22.4 1.0 O C:HOH402 3.9 22.7 1.0 N C:CYS41 3.9 27.6 1.0 CA C:CYS24 4.1 22.7 1.0 O C:HOH400 4.2 22.8 1.0 CA C:CYS41 4.3 26.4 1.0 N C:CYS44 4.3 23.3 1.0 CA C:CYS44 4.3 23.1 1.0 CB C:ASP23 4.6 23.2 1.0 CA C:CYS21 4.7 22.0 1.0 CB C:ALA26 4.7 21.9 1.0 C C:CYS24 4.7 22.4 1.0 C C:ASP23 4.7 23.5 1.0 N C:GLY25 4.8 23.4 1.0 C C:CYS41 4.9 25.1 1.0 N C:ALA26 4.9 25.2 1.0 N C:ASP23 5.0 24.0 1.0 O C:CYS41 5.0 23.1 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of Full Length Centaurin Alpha-1 Bound with the Fha Domain of KIF13B (Capri Target)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Full Length Centaurin Alpha-1 Bound with the Fha Domain of KIF13B (Capri Target) within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn401 b:44.1 occ:1.00 SG D:CYS24 2.3 42.7 1.0 SG D:CYS21 2.3 40.3 1.0 SG D:CYS44 2.3 40.3 1.0 SG D:CYS41 2.3 45.5 1.0 CB D:CYS21 3.3 42.5 1.0 CB D:CYS44 3.3 41.6 1.0 CB D:CYS24 3.3 41.4 1.0 CB D:CYS41 3.5 44.9 1.0 N D:CYS24 3.7 37.4 1.0 N D:CYS41 4.0 46.5 1.0 CA D:CYS24 4.0 40.5 1.0 CA D:CYS41 4.3 46.1 1.0 N D:CYS44 4.3 43.2 1.0 CA D:CYS44 4.4 41.2 1.0 CB D:ALA26 4.7 51.0 1.0 CA D:CYS21 4.7 43.3 1.0 C D:CYS24 4.7 42.4 1.0 N D:GLY25 4.7 43.5 1.0 C D:ASP23 4.7 35.7 1.0 CB D:ASP23 4.8 32.3 1.0 N D:ALA26 4.9 50.7 1.0 C D:CYS41 4.9 48.1 1.0 N D:ASP23 4.9 34.3 1.0

Y.Tong, W.Tempel, H.Wang, K.Yamada, L.Shen, G.A.Senisterra, F.Mackenzie, A.H.Chishti, H.W.Park. Phosphorylation-Independent Dual-Site Binding of the Fha Domain of KIF13 Mediates Phosphoinositide Transport Via Centaurin ALPHA1. Proc.Natl.Acad.Sci.Usa V. 107 20346 2010.
ISSN: ISSN 0027-8424
PubMed: 21057110
DOI: 10.1073/PNAS.1009008107