Zinc in PDB 3fc5: G586S Mutant Nnosoxy

All present enzymatic activity of G586S Mutant Nnosoxy:
1.14.13.39;

The structure of G586S Mutant Nnosoxy, PDB code: 3fc5 was solved by C.Bruckmann, C.G.Mowat, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	34.27 / 2.59
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	51.623, 110.276, 164.671, 90.00, 90.00, 90.00
R / Rfree (%)	19.9 / 25.4

The structure of G586S Mutant Nnosoxy also contains other interesting chemical elements:

Iron

(Fe)

2 atoms

The binding sites of Zinc atom in the G586S Mutant Nnosoxy (pdb code 3fc5). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the G586S Mutant Nnosoxy, PDB code: 3fc5:

Zinc binding site 1 out of 1 in the G586S Mutant Nnosoxy


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of G586S Mutant Nnosoxy within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn900 b:47.5 occ:1.00 SG B:CYS331 2.3 49.2 1.0 SG A:CYS331 2.3 42.8 1.0 SG B:CYS326 2.4 50.8 1.0 SG A:CYS326 2.4 49.5 1.0 CB B:CYS331 3.1 54.4 1.0 CB A:CYS331 3.2 55.3 1.0 CA B:CYS331 3.5 53.2 1.0 CB B:CYS326 3.6 54.9 1.0 CB A:CYS326 3.6 55.1 1.0 CA A:CYS331 3.6 54.1 1.0 N B:MET332 4.0 44.4 1.0 N B:GLY333 4.0 42.6 1.0 C B:CYS331 4.1 51.4 1.0 N A:MET332 4.1 47.8 1.0 N A:GLY333 4.1 45.2 1.0 C A:CYS331 4.2 53.3 1.0 CA B:GLY333 4.4 40.5 1.0 CA A:GLY333 4.5 39.5 1.0 N B:CYS331 4.8 52.6 1.0 CA B:CYS326 4.9 55.6 1.0 N A:CYS331 4.9 48.2 1.0 CA A:CYS326 4.9 58.7 1.0 C B:MET332 4.9 49.6 1.0 O B:ILE330 5.0 57.5 1.0

D.Papale, C.Bruckmann, C.S.Miles, C.G.Mowat, S.Daff. Oxygen Activation in Neuronal No Synthase: Stabilisation of A Novel Intermediate in the G586S Mutant To Be Published.