Zinc in PDB 3eqo: Crystal Structure of Beta-1,3-Glucanase From Phanerochaete Chrysosporium (LAM55A) Gluconolactone Complex

All present enzymatic activity of Crystal Structure of Beta-1,3-Glucanase From Phanerochaete Chrysosporium (LAM55A) Gluconolactone Complex:
3.2.1.58;

The structure of Crystal Structure of Beta-1,3-Glucanase From Phanerochaete Chrysosporium (LAM55A) Gluconolactone Complex, PDB code: 3eqo was solved by T.Ishida, S.Fushinobu, R.Kawai, M.Kitaoka, K.Igarashi, M.Samejima, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	31.50 / 2.25
Space group	P 1
Cell size a, b, c (Å), α, β, γ (°)	66.534, 67.146, 109.778, 93.94, 106.76, 97.08
R / Rfree (%)	14.2 / 20

The binding sites of Zinc atom in the Crystal Structure of Beta-1,3-Glucanase From Phanerochaete Chrysosporium (LAM55A) Gluconolactone Complex (pdb code 3eqo). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of Beta-1,3-Glucanase From Phanerochaete Chrysosporium (LAM55A) Gluconolactone Complex, PDB code: 3eqo:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of Beta-1,3-Glucanase From Phanerochaete Chrysosporium (LAM55A) Gluconolactone Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Beta-1,3-Glucanase From Phanerochaete Chrysosporium (LAM55A) Gluconolactone Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn756 b:38.8 occ:1.00 NE2 A:HIS383 2.2 12.3 1.0 O A:HOH1388 2.8 21.0 1.0 CD2 A:HIS383 3.1 13.2 1.0 CE1 A:HIS383 3.3 11.9 1.0 OD2 A:ASP488 3.4 24.4 1.0 O A:PRO384 3.6 20.5 1.0 OG1 A:THR487 3.6 14.9 1.0 CB A:THR487 3.9 15.6 1.0 NH1 A:ARG154 4.1 13.7 1.0 O A:HOH1378 4.2 37.1 1.0 CG A:HIS383 4.3 14.0 1.0 ND1 A:HIS383 4.3 13.5 1.0 CG1 A:VAL449 4.4 15.2 1.0 CG2 A:THR487 4.5 14.6 1.0 C A:PRO384 4.6 19.8 1.0 CG A:ASP488 4.6 21.0 1.0 CA A:GLN385 4.6 19.7 1.0 CG A:GLN385 4.9 22.2 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of Beta-1,3-Glucanase From Phanerochaete Chrysosporium (LAM55A) Gluconolactone Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Beta-1,3-Glucanase From Phanerochaete Chrysosporium (LAM55A) Gluconolactone Complex within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn756 b:39.4 occ:1.00 O B:HOH1346 2.1 22.8 1.0 NE2 B:HIS383 2.2 20.3 1.0 O B:HOH1343 2.8 21.1 1.0 CD2 B:HIS383 3.1 18.7 1.0 CE1 B:HIS383 3.2 16.6 1.0 OD2 B:ASP488 3.3 22.6 1.0 OG1 B:THR487 3.5 18.0 1.0 O B:PRO384 3.7 17.6 1.0 CB B:THR487 3.8 19.2 1.0 O B:HOH1345 3.9 40.6 1.0 NH1 B:ARG154 4.0 25.7 1.0 CG1 B:VAL449 4.3 20.8 1.0 ND1 B:HIS383 4.3 12.4 1.0 CG B:HIS383 4.3 17.1 1.0 CG2 B:THR487 4.4 19.0 1.0 CG B:ASP488 4.5 20.7 1.0 CG B:GLN385 4.7 21.9 1.0 C B:PRO384 4.8 19.4 1.0 CA B:GLN385 4.9 20.9 1.0

T.Ishida, S.Fushinobu, R.Kawai, M.Kitaoka, K.Igarashi, M.Samejima. Crystal Structure of Glycoside Hydrolase Family 55 Beta -1,3-Glucanase From the Basidiomycete Phanerochaete Chrysosporium J.Biol.Chem. V. 284 10100 2009.
ISSN: ISSN 0021-9258
PubMed: 19193645
DOI: 10.1074/JBC.M808122200