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Zinc in PDB 3eg9: Crystal Structure of the Mammalian Copii-Coat Protein SEC23/24 Bound to the Transport Signal Sequence of Membrin

Protein crystallography data

The structure of Crystal Structure of the Mammalian Copii-Coat Protein SEC23/24 Bound to the Transport Signal Sequence of Membrin, PDB code: 3eg9 was solved by J.Goldberg, J.D.Mancias, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 50.00 / 3.00
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 103.130, 140.750, 152.420, 90.00, 90.00, 90.00
R / Rfree (%) 20.2 / 25.4

Zinc Binding Sites:

The binding sites of Zinc atom in the Crystal Structure of the Mammalian Copii-Coat Protein SEC23/24 Bound to the Transport Signal Sequence of Membrin (pdb code 3eg9). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of the Mammalian Copii-Coat Protein SEC23/24 Bound to the Transport Signal Sequence of Membrin, PDB code: 3eg9:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in 3eg9

Go back to Zinc Binding Sites List in 3eg9
Zinc binding site 1 out of 2 in the Crystal Structure of the Mammalian Copii-Coat Protein SEC23/24 Bound to the Transport Signal Sequence of Membrin


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of the Mammalian Copii-Coat Protein SEC23/24 Bound to the Transport Signal Sequence of Membrin within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn800

b:45.1
occ:1.00
SG A:CYS61 2.3 44.8 1.0
SG A:CYS85 2.3 43.3 1.0
SG A:CYS88 2.3 42.6 1.0
SG A:CYS66 2.4 49.7 1.0
CB A:CYS61 3.0 37.8 1.0
CB A:CYS85 3.1 39.8 1.0
CB A:CYS66 3.2 45.8 1.0
CB A:CYS88 3.7 43.1 1.0
N A:CYS88 4.0 49.0 1.0
CB A:GLN90 4.3 50.7 1.0
CA A:CYS88 4.3 49.7 1.0
N A:ARG67 4.4 47.2 1.0
CA A:CYS61 4.5 42.0 1.0
CA A:CYS85 4.5 39.9 1.0
CA A:CYS66 4.6 44.0 1.0
CB A:ARG63 4.6 49.3 1.0
N A:ALA68 4.6 43.4 1.0
CB A:PHE87 4.8 37.8 1.0
C A:CYS88 4.8 51.8 1.0
CB A:ALA68 4.8 44.0 1.0
C A:CYS66 4.9 46.2 1.0
C A:PHE87 4.9 43.4 1.0
N A:GLN90 5.0 56.4 1.0

Zinc binding site 2 out of 2 in 3eg9

Go back to Zinc Binding Sites List in 3eg9
Zinc binding site 2 out of 2 in the Crystal Structure of the Mammalian Copii-Coat Protein SEC23/24 Bound to the Transport Signal Sequence of Membrin


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of the Mammalian Copii-Coat Protein SEC23/24 Bound to the Transport Signal Sequence of Membrin within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn1100

b:57.4
occ:1.00
SG B:CYS367 2.3 42.0 1.0
SG B:CYS389 2.3 52.3 1.0
SG B:CYS386 2.4 59.0 1.0
SG B:CYS364 2.5 55.9 1.0
CB B:CYS386 3.0 61.5 1.0
CB B:CYS364 3.2 53.4 1.0
N B:CYS389 3.7 54.9 1.0
CB B:CYS389 3.7 54.8 1.0
CB B:CYS367 3.8 42.2 1.0
N B:CYS367 4.0 48.3 1.0
CA B:CYS389 4.3 54.6 1.0
CB B:PHE388 4.4 44.5 1.0
CA B:CYS367 4.4 44.0 1.0
CA B:CYS386 4.4 60.3 1.0
C B:PHE388 4.6 51.1 1.0
N B:PHE388 4.6 48.8 1.0
CB B:CYS391 4.7 71.0 1.0
CA B:CYS364 4.7 54.9 1.0
CB B:ARG366 4.7 60.6 1.0
CA B:PHE388 4.7 47.6 1.0
C B:CYS386 4.8 60.6 1.0
C B:CYS389 4.8 54.9 1.0
N B:CYS391 4.8 67.5 1.0
N B:ASN390 4.8 59.2 1.0
CB B:ALA369 5.0 49.8 1.0
N B:LYS368 5.0 43.0 1.0
C B:CYS367 5.0 42.5 1.0

Reference:

J.D.Mancias, J.Goldberg. Structural Basis of Cargo Membrane Protein Discrimination By the Human Copii Coat Machinery. Embo J. V. 27 2918 2008.
ISSN: ISSN 0261-4189
PubMed: 18843296
DOI: 10.1038/EMBOJ.2008.208
Page generated: Wed Dec 16 04:15:54 2020

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